HIV-1 drug resistance genotyping from dried blood spots stored for 1 year at 4°C

Journal of Antimicrobial Chemotherapy

Volumn 61, Issue 6, 2008, Pages 1217-1220

  Youngpairoj, Ae S ^a   Masciotra, Silvina ^a   Garrido, Carolina ^b   Zahonero, Natalia ^b   De Mendoza, Carmen ^b   García Lerma, J Gerardo ^a  

^a CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

^b HOSPITAL UNIVERSITARIO LA PAZ   (Spain)

Author keywords

903 filter paper; Resistance testing; ViroSeq assay

Indexed keywords

ANTIRETROVIRUS AGENT; POL PROTEIN;

ARTICLE; ASSAY; BLOOD SAMPLING; BLOOD STORAGE; CD4+ T LYMPHOCYTE; CONTROLLED STUDY; GENE AMPLIFICATION; GENE SEQUENCE; GENOMIC FRAGMENT; GENOTYPE; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION; INCUBATION TEMPERATURE; INCUBATION TIME; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA COPIES; SEQUENCE ANALYSIS; VIRUS GENE; VIRUS RESISTANCE;

BLOOD; DESICCATION; DRUG RESISTANCE; GENOTYPE; HIV INFECTIONS; HIV-1; HUMANS; MICROBIAL SENSITIVITY TESTS; NUCLEIC ACIDS; REFRIGERATION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING; TIME FACTORS;

EID: 44449128907     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dkn100     Document Type: Article

References (10)

1. Solomon SS, Pulimi S, Rodriguez II et al. Dried blood spots are an acceptable and useful HIV surveillance tool in an remote developing world setting. Int J STD AIDS 2004; 15: 658-61.
2. Beck IA, Drennan KD, Melvin AJ et al. Simple, sensitive, and specific detection of human immunodeficiency virus type 1 subtype B DNA in dried blood samples for diagnosis in infants in the field. J Clin Microbiol 2001; 39: 29-33.
3. Brambilla D, Jennings C, Aldrovandi G et al. Multicenter evaluation of use of dried blood and plasma spot specimens in quantitative assays or human immunodeficiency virus RNA: Measurement, precision, and RNA stability. J Clin Microbiol 2003; 41: 1888-93.
4. Fiscus SA, Brambilla, D. Grosso L et al. Quantitation of human immunodeficiency virus type 1 RNA in plasma by using blood dried on filter paper. J Clin Microbiol 1998; 36: 258-60.
5. O'Shea S, Mullen J, Corbett K et al. Use of dried whole blood spots for qunatification of HIV-1 RNA. AIDS 1999; 13: 630.
6. Masciotra S, Garrido, C, Youngpairoj Ae S et al. High concordance between HIV-1 drug resistanc genotypes generated from plasma and dried blood spots in antiretroviral-experienced patients. AIDS 2007; 21: 2503-11.
7. McNutty A, Jennings C, Bennett D et al. Evaluation of dried blood spots for HIV-1 drug resistance testing. J Clin Microbiol 2007; 45: 517-21.
8. Alvarez-Muñoz MT, Zaragoza-Rodríguez S, Rojas-Montes O et al High correlation of human immunodeficiency virus type-1 viral load measured in dried-blood spot samples and in plasma under different storage conditions. Arch Med Res 2005; 36: 382-6.
9. Cassol S, Gill MJ, Pilon R et al. Quantification of human immunodeficiency virus type 1 RNA from dried plasma spots collected on filter paper. J Clin Microbiol 1997; 35: 2795-801.
10. Bertagnolio S, Soto-Ramirez L, Pilon R et al. HIV-1 drug resistance surveillance using dried whole blood spots. Antiviral Ther 2007; 12: 107-13.