Generation of amylosucrase variants that terminate catalysis of acceptor elongation at the di- or trisaccharide stage

Applied and Environmental Microbiology

Volumn 75, Issue 23, 2009, Pages 7453-7460

  Schneider, Jens ^a,b   Fricke, Christin ^a   Overwin, Heike ^a   Hofmann, Birgit ^a   Hofer, Bernd ^a  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^b Federal Research Institute of Rural Areas Forestry and Fisheries vTI   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ACCEPTOR MOLECULES; AMINO ACID SUBSTITUTION; CATALYTIC ACTIVITY; CHAIN ELONGATION; DONOR SUBSTRATES; HIGH RATE; MUTATION RATES; RANDOM MUTAGENESIS; STERIC INTERFERENCE; STRUCTURAL MODELS; SUBSITES;

AMINATION; AMINES; ELONGATION; ENZYMES; GENE ENCODING; GLUCOSE; MODEL STRUCTURES; ORGANIC ACIDS; SUGAR (SUCROSE);

AMINO ACIDS;

AMYLOSUCRASE; DISACCHARIDE; ENZYME VARIANT; GLUCAN; GLUCOSE; OLIGOSACCHARIDE; SUCRASE; TRISACCHARIDE; UNCLASSIFIED DRUG;

AMINO ACID; BIOCHEMISTRY; CARBOHYDRATE; CATALYSIS; CATALYST; ENZYME ACTIVITY; GENE EXPRESSION; MUTATION; SUBSTRATE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CODON; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME STRUCTURE; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENE MUTATION; MOLECULAR CLONING; MUTAGENESIS; MUTATION RATE; NONHUMAN; NUCLEOTIDE SEQUENCE; STEREOCHEMISTRY;

AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; BINDING SITES; DISACCHARIDASES; GLUCOSYLTRANSFERASES; MODELS, MOLECULAR; MUTAGENESIS; MUTANT PROTEINS; NEISSERIA; PROTEIN STRUCTURE, TERTIARY; TRISACCHARIDES;

EID: 72949120604     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01194-09     Document Type: Article

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