Expression, purification and characterization of recombinant Jerdonitin, a P-II class snake venom metalloproteinase comprising metalloproteinase and disintegrin domains

Toxicon

Volumn 55, Issue 2-3, 2010, Pages 375-380

  Zhu, Lili ^a,b   Yuan, Cai ^a,b   Chen, Zhuo ^a,b   Wang, Wanyu ^b,c   Huang, Mingdong ^a,b  

^a FUJIAN INSTITUTE OF RESEARCH ON THE STRUCTURE OF MATTER   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c KUNMING INSTITUTE OF ZOOLOGY   (China)

Author keywords

Disintegrin; Fibrinolysis activity; Jerdonitin; Metalloproteinase; MTT colorimetric assay; Pichia pastoris; Platelet aggregation; Recombinant protein expression; Snake venom metalloproteinases; Tumor cells

Indexed keywords

ADENOSINE DIPHOSPHATE; DISINTEGRIN; FIBRINOGEN; METALLOPROTEINASE; RECOMBINANT JERDONITIN; SNAKE VENOM; UNCLASSIFIED DRUG;

ARTICLE; CANCER CELL CULTURE; CANCER INHIBITION; CONTROLLED STUDY; CYTOTOXICITY; ENZYME ANALYSIS; ENZYME PURIFICATION; FIBRINOLYSIS; HUMAN; HUMAN CELL; IC 50; LEUKEMIA CELL; LIVER CANCER; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; STOMACH CARCINOMA; THROMBOCYTE AGGREGATION INHIBITION; WILD TYPE;

ANIMALS; CELL LINE, TUMOR; COLORING AGENTS; DISINTEGRINS; DNA, COMPLEMENTARY; DOSE-RESPONSE RELATIONSHIP, DRUG; FIBRINOLYTIC AGENTS; HUMANS; METALLOPROTEASES; PICHIA; PLASMIDS; PLATELET AGGREGATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; SNAKE VENOMS; TETRAZOLIUM SALTS; THIAZOLES;

PICHIA PASTORIS;

EID: 72749097175     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxicon.2009.08.016     Document Type: Article

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