The effects of the physical properties of highly compacted smectitic clay (bentonite) on the culturability of indigenous microorganisms

Applied Clay Science

Volumn 47, Issue 1-2, 2010, Pages 155-162

  Stroes Gascoyne, S ^a   Hamon, C J ^a   Maak, P ^b   Russell, S ^b  

^a ATOMIC ENERGY OF CANADA LIMITED   (Canada)

^b Nuclear Waste Management Organization   (Canada)

Author keywords

Bentonite; Dry density; Microbial activity; Porewater salinity; Swelling pressure; Water activity

Indexed keywords

DRY DENSITY; MICROBIAL ACTIVITIES; PORE WATERS; SWELLING PRESSURES; WATER ACTIVITY;

CLAY MINERALS; CONTAINERS; CORROSION; FATTY ACIDS; NUCLEAR ENERGY; NUCLEAR FUELS; PHOSPHOLIPIDS; POWDERS; SALINITY MEASUREMENT; SWELLING; WASTE MANAGEMENT;

BENTONITE;

BENTONITE; COMPACTION; DRY DENSITY; MICROBIAL ACTIVITY; POREWATER; SALINITY; SMECTITE; SWELLING;

EID: 72649099792     PISSN: 01691317     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.clay.2008.06.010     Document Type: Article

References (29)

1. Atlas R.M. In: Parks L.C. (Ed). Handbook of Microbiological Media (1993), CRC Press Inc
2. Boivin-Jahns V., Ruimy R., Bianchi A., Daumas S., and Christen R. Bacterial diversity in a deep-subsurface clay environment. Appl. Environ. Microbiol. 62 (1996) 3405-3412
3. Brown A.D. Microbial Water Stress Physiology (1990), John Wiley, Chichester, UK
4. Chapelle F.H. Ground-water Microbiology and Geochemistry (1993), John Wiley and Sons, New York, NY
5. Choi J.-W., and Oscarson D.W. Diffusive transport through compacted Na- and Ca-bentonite. J. Contam. Hydrol. 22 (1996) 189-202
6. Fredrickson J.K., McKinley J.P., Bjornstad B.N., Long P.E., Ringelberg D.B., White D.C., Krumholtz L.R., Suflita J.M., Colwell F.S., Lehman R.M., Phelps T.J., and Onstott T.C. Pore-size constraints on the activity and survival of subsurface bacteria in late Cretaceous hale-sandstone sequence, northwest New Mexico. Geomicrobiol. J. 14 (1997) 183-202
7. Fukunaga S., Jintoku T., Iwata Y., and Nakayama M. Investigation of microorganisms in bentonite deposits. Geomicrobiol. J. 22 (2005) 361-370
8. Hallberg R.O., Östlund P., and Wadsten T. Inferences from a corrosion study of a bronze cannon, applied to high level nuclear waste disposal. Appl. Geochem. 3 (1988) 273-280
9. Hedin A. Safety function indicators in SKB's safety assessments of a KBS-3 repository. Proc. International High-Level Radioactive Waste Management Conference, Las Vegas, NV, April 30-May 04, 2006 (2006)
10. Johnson S.S., Hebsgaard M.B., Christensen T.R., Mastepanov M., Nielsen R., Munch K., Brand T., Gilbert M.T.P., Zuber M.T., Brunce M., Ronn R., Gillichinsky D., Froese D., and Willerslev E. Ancient bacteria show evidence of DNA repair. Proc. Natl. Acad. Sci. 104 (2007) 14401-14405
11. King F. A natural analogue for the long-term corrosion of copper nuclear waste containers - reanalysis of a study of a bronze cannon. Appl. Geochem. 10 (1995) 477-487
12. Kjartanson, B.H., Dixon, D.A., Stroes-Gascoyne, S. 2003. Effects of container/buffer gap fill on buffer performance. Ontario Power Generation Report 06819-REP-01200-10102-R00.
13. Krumholtz L.R., McKinley J.P., Ulrich G.A., and Suflita J.M. Confined subsurface microbial communities in Cretaceous rock. Nature 386 (1997) 64-66
14. McDougald D., Rice S.A., Weichart D., and Kjelleberg S. Nonculturability: adaptation or debilitation?. FEMS Microbiol. Ecol. 25 (1998) 1-9
15. Miller W., Alexander R., Chapman N., McKinley I., and Smellie J.T. Geological disposal of radioactive wastes and natural analogues. Lessons from Nature and Archaeology (2000), Elsevier/Pergamon, Amsterdam
16. Milodowski A.E., Styles M.T., Werme L., and Oversby V.M. The corrosion of more than 176 million year old native copper plates from a deposit in mudstone in south Devon (2003), Corrosion, United Kingdom Paper 03681, NACE International
17. NWMO. Choosing a way forward. The future management of Canada's used nuclear fuel (2005), Nuclear Waste Management Organization, Toronto, Canada
18. Onstott T.C., Phelps T.J., Colwell F.S., Ringelberg D., White D.C., Boone D.R., McKinley J., Stevens T.O., Long P.E., Balkwill D.L., Griffin W.T., and Kieft T. Observations pertaining to the origin and ecology of microorganisms recovered from the deep subsurface of Taylorsville Basin, Virginia. Geomicrobiology 15 (1998) 353-385
19. Pedersen K., Motamedi M., Karnland O., and Sanden T. Cultivability of micro-organisms introduced into a compacted bentonite clay buffer under high-level radioactive waste repository conditions. Eng. Geol. 58 2 (2000) 149-161
20. Potts M. Desiccation tolerance of prokaryotes. Microbiol Rev 58 (1994) 755-805
21. Reasoner D.L., and Geldreich E.E. A new medium for the enumeration and subculture of bacteria from potable water. Appl. Environ. Microbiol. 1 (1985) 1-7
22. Stroes-Gascoyne S., Hamon C.J., Dixon D.A., and Martino J.B. Microbial analysis of samples from the tunnel sealing experiment at AEL's Underground Research Laboratory. Phys. Chem. Earth. 32 (2007) 219-231
23. Stroes-Gascoyne S., Schippers A., Schwyn B., Poulain S., Sergeant C., Le Marrec C., Simonoff M., Altmann S., Nagaoka T., Mauclaire L., McKenzie J., Daumas S., Vinsot A., Beauclaire C., and Matray J.M. Microbial community analysis of Opalinus clay drill core samples from the Mont Terri Underground Research Laboratory, Switzerland. Geomicrobiol. J. 24 (2007) 1-17
24. Stroes-Gascoyne S., Hamon C.J., Kohle C., and Dixon D.A. The effects of dry density and porewater salinity on the physical and microbiological characteristics of highly compacted bentonite. Ontario Power Generation Report 06819-REP-01200-10016-R00. Pinawa, Manitoba (2006)
25. Stroes-Gascoyne S., Hamon C.J., Vilks P., and Gierszewski P. Microbial, redox and organic characteristics of compacted clay-based buffer after 6.5 years of burial at AECL's Underground Research Laboratory. Appl. Geochem. 17 (2002) 1287-1303
26. Stroes-Gascoyne S., and King F. Microbially influenced corrosion issues in high-level nuclear waste repositories. In: Little B., and Chair (Eds). Proceedings of CORROSION/2002 Research Topical Symposium Microbially Influenced Corrosion (2002), NACE International, Houston, TX 79
27. Stroes-Gascoyne S., Pedersen K., Haveman S.A., Daumas S., Hamon C.J., Arlinger J., Ekendahl S., Hallbeck L., Gahroni, N., Delaney T.L., and Dekeyser K. Occurrence and identification of microorganisms in compacted clay-based buffer material designed for use in a nuclear fuel waste disposal vault. Can. J. Microbiol. 43 (1997) 1133-1146
28. Stroes-Gascoyne, S., Pedersen, K., Daumas, S., Hamon, C., Haveman, S., Delaney, T., Ekendahl, S., Arlinger, J., Hallbeck, L., Dekeyser, K. 1996. Microbial analysis of the buffer/container experiment at AECL's Underground Research Laboratory. Atomic Energy of Canada Limited Report, AECL-11436, COG-95-446.
29. White D.C., and Ringelberg D.B. Utility of the signature lipid biomarker analysis in determining the in situ viable biomass, community structure, and nutritional/physiological status of deep subsurface microbiota. In: Amy P.S., and Haldeman D.L. (Eds). Microbiol Terr Deep Subsurf (1997), Lewis Publishers, Boca Raton, USA