Microbial analysis of samples from the tunnel sealing experiment at AECL's Underground Research Laboratory

Physics and Chemistry of the Earth

Volumn 32, Issue 1-7, 2007, Pages 219-231

  Stroes Gascoyne, S ^a   Hamon, C J ^a   Dixon, D A ^a   Martino, J B ^a  

^a ATOMIC ENERGY OF CANADA LIMITED   (Canada)

Author keywords

Bentonite; Bulkhead; Clay; Microbes; Seal; Underground Research Laboratory

Indexed keywords

INTERFACES (MATERIALS); MICROBIOLOGY; MOISTURE; PORE SIZE; REDOX REACTIONS;

CLAY SEAL; MICROBIAL ACTIVITY; PORE SPACE; TUNNEL SEALING EXPERIMENT;

CLAY;

BACKFILL; BENTONITE; DECOMMISSIONING; DENSITY; EXPERIMENTAL STUDY; MICROBIAL ACTIVITY; MOISTURE; POROSITY; REDOX CONDITIONS; SEALING; TUNNEL;

EID: 33847742254     PISSN: 14747065     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pce.2006.01.002     Document Type: Article

References (19)

1. Amann R.I., Ludwig W., and Schleifer K.H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59 (1995) 143-169
2. Atlas R.M. In: Parks L.C. (Ed). Handbook of Microbiological Media (1993), CRC Press
3. Brown A.D. Microbial Water Stress Physiology (1990), John Wiley, Chichester, UK
4. Chandler, N., Cournut, A, Dixon, D., Fairhurst, C., Hansen, F., Gray, M., Hara, K., Ishijima, Y., Kozak, E., Martino, J., Masumoto, K., McCrank, G., Sugita, Y., Thompson, P., Tillerson, J., Vignal, B., 2002. The five year report of the tunnel sealing experiment: an international project of AECL, JNC, ANDRA and WIPP. Atomic Energy of Canada Limited Report, AECL-12727.
5. Dixon, D.A., Martino, J.B., Chandler, N.A., Guo, R., 2004. Performance of the clay bulkhead portion of the tunnel sealing experiment under ambient temperature conditions. Ontario Power Generation Report 06819-REP-01200-10131.
6. Fredrickson J.K., McKinley J.P., Bjornstad B.N., Long P.E., Ringelberg D.B., White D.C., Krumholtz L.R., Suflita J.M., Colwell F.S., Lehman R.M., Phelps T.J., and Onstott T.C. Pore-size constraints on the activity and survival of subsurface bacteria in a late Cretaceous shale-sandstone sequence, Northwestern New Mexico. Geomicrobiol. J. 14 (1997) 183-202
7. Gerhardt P., Murray R.G.E., Wood W.A., and Krieg N.R. Methods for general and molecular bacteriology (1994), American Society for Microbiology, Washington, DC, USA
8. Kjartanson, B.H., Dixon, D.A., Stroes-Gascoyne, S., 2003. Effects of container/buffer gap fill on buffer performance. Ontario Power Generation Report, 06819-REP-01200-10102-R00.
9. Martino, J.B., Chandler, N.A., Dixon, D.A., Sugita, Y., Vignal, B., Hansen, F., 2003. The tunnel sealing experiment and application to repository seal design. In: Proceedings of the 10th International High-Level Radioactive Waste Management Conference, Las Vegas, NV, March 31-April 03, 2003.
10. Pedersen K. Microbial processes in the disposal of high level radioactive waste 500 m underground in fennoscandian shield rock. In: Keith-Roach M.J., and Livens F.R. (Eds). Microbiology and Radioactivity (2002), Elsevier Science
11. Pedersen, K., Motamedi, M., Karnland, O., 1995. Survival of bacteria in nuclear waste buffer materials; the influence of nutrients, temperature and water activity. SKB Technical Report 95-27. Swedish Nuclear Fuel and Waste Management Co. Stockholm, Sweden.
12. Reasoner D.L., and Geldreich E.E. A new medium for the enumeration and subculture of bacteria from potable water. Appl. Environ. Microbiol. 1 (1985) 1-7
13. Stroes-Gascoyne S., and West J.M. Microbial studies in the Canadian nuclear fuel waste management program. FEMS Microbiol. Rev. 20 (1997) 573-590
14. Stroes-Gascoyne S., and Sargent F.P. The Canadian approach to microbial studies in nuclear waste management and disposal. Cont. Hydrol. 35 (1998) 175-190
15. Stroes-Gascoyne S., Pedersen K., Haveman S.A., Daumas S., Hamon C.J., Arlinger J., Ekendahl S., Hallbeck L., Gahroni N., Delaney T.L., and Dekeyser K. Occurrence and identification of microorganisms in compacted clay-based buffer material designed for use in a nuclear fuel waste disposal vault. Can. J. Microbiol. 43 (1997) 1133-1146
16. Stroes-Gascoyne, S., Lucht, L.M., Oscarson, D.W., Dixon, D.A., Hume, H.B., Miller, S.H., 1999. Migration of bacteria in compacted clay-based material. In: Kodama, H., Mermut, A.R., Torrance, J.K. (Eds.), Clays for our Future, Proceedings of the 11th International Clay Conference, ISBN 0-96863 14-0-7. Ottawa, ON, 1997 June 15-21, pp. 117-122.
17. Stroes-Gascoyne S., Hamon C.J., Vilks P., and Gierszewski P. Microbial, redox and organic characteristics of compacted clay-based buffer after 6.5 years of burial at AECL's Underground Research Laboratory. Appl. Geochem. 17 (2002) 1287-1303
18. Stroes-Gascoyne, S., Hamon, C.J., Dixon, D.A., Kjartanson, B., 2005. Factors affecting microbial activity in compacted clay-based sealing materials proposed for use in a deep geologic repository for used nuclear fuel. In: Proceedings of the CNS Waste Management, Decommissioning and Environmental Restoration Conference, May 8-12, Ottawa, Canada.
19. West J.M., McKinley I.G., and Stroes-Gascoyne S. Microbial effects on waste repository materials. In: Keith-Roach M.J., and Livens F.R. (Eds). Microbiology and Radioactivity (2002), Elsevier Science