Rapid detection of starved escherichia coli with respiratory activity in potable water by signal-amplified in situ hybridization following formazan reduction

Microbes and Environments

Volumn 24, Issue 4, 2009, Pages 286-290

  Yamaguchi, Nobuyasu ^a   Sasada, Makoto ^a   Nasu, Masao ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

Fecal indicator; Fluorescence in situ hybridization; Microbial monitoring; Potable water; Respiratory activity

Indexed keywords

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 72549102732     PISSN: 13426311     EISSN: 13426311     Source Type: Journal    
DOI: 10.1264/jsme2.ME09144     Document Type: Article

References (34)

1. Amann, R.I., B.J. Binder, R.J. Olson, S.W. Chisholm, R. Devereux, and D.A. Stahl. 1990. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 56:1919-1925.
2. Amann, R.I., W. Ludwig, and K.H. Schleifer. 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59:143-169.
3. Amor, K.B., P. Breeuwer, P. Verbaarschot, F.M. Rombouts, A.D.L. Akkermans, W.M. de Vos, and T. Abee. 2002. Multiparametric flow cytometry and cell sorting for the assessment of viable, injured, and dead bifidobacterium cells during bile salt stress. Appl. Environ. Microbiol. 68:5209-5216.
4. Baba, T., R. Matsumoto, N. Yamaguchi, and M. Nasu. 2009. Bacterial population dynamics in a reverse-osmosis water purification system determined by fluorescent staining and PCR-denaturing gradient gel electrophoresis. Microbes Environ. 24:163-167.
5. Chmielewski, R.A.N., and J.F. Frank. 1995. Formation of viable but nonculturable Salmonella during starvation in chemically defined solutions. Lett. Appl. Microbiol. 20:380-384.
6. Dive, C., H. Cox, J.V.W. Watson, and P. Workman. 1988. Polar fluorescein derivatives as improved substrate probes for flow cytoenzymological assay of cellular esterases. Mol. Cell. Probes 2:131-145.
7. Fujii, Y., and A. Hiraishi. 2009. Combined use of cyanoditolyl tetrazolium staining and flow cytometry for detection of metabolically active bacteria in a fed-batch composting process. Microbes Environ. 24:57-63.
8. Fukushima, T., N. Uda, M. Okamoto, M. Onuki, H. Satoh, and T. Mino. 2007. Abundance of Candidatus 'Accumulibacter phosphatis' in enhanced biological phosphorus removal activated sludge acclimatized with different carbon sources. Microbes Environ. 22:346-354.
9. Jansen, G.J., A.C.M. Wildeboer-Veloo, R.H.J. Tonk, A.H. Franks, and G.W. Welling. 1999. Development and validation of an automated microscopy-based method for enumeration of groups of intestinal bacteria. J. Microbiol. Methods 37:215-221.
10. Jiang, X., and T.-J. Chai. 1996. Survival of Vibrio parahaemolyticus at low temperature under starvation conditions and subsequent resuscitation of viable, nonculturable cells. Appl. Environ. Microbiol. 62:1300-1305.
11. Jones, I.G., and M. Roworth. 1996. An outbreak of Escherichia coli O157 and campylobacteriosis associated with contamination of a drinking water supply. Public Health 110:277-282.
12. Kawai, M., N. Yamaguchi, and M. Nasu. 1999. Rapid enumeration of physiologically active bacteria in purified water used in the pharmaceutical manufacturing process. J. Appl. Microbiol. 86:496-504.
13. Kenzaka, T., N. Yamaguchi, K. Tani, and M. Nasu. 1998. rRNA-targeted fluorescent in situ hybridization analysis of bacterial community structure in river water. Microbiology 144:2085-2093.
14. Kenzaka, T., N. Yamaguchi, B. Prapagdee, E. Mikami, and M. Nasu. 2001. Bacterial community composition and activity in urban rivers in Thailand and Malaysia. J. Health Sci. 47:353-361.
15. Kitaguchi, A., N. Yamaguchi, and M. Nasu. 2005. Enumeration of respiring Pseudomonas spp. in milk within 6 hours by fluorescence in situ hybridization following formazan reduction. Appl. Environ. Microbiol. 71:2748-2752.
16. Kitaguchi, A., N. Yamaguchi, and M. Nasu. 2006. Simultaneous enumeration of viable Enterobacteriaceae and Pseudomonas spp. within three hours by multicolor fluorescence in situ hybridization with vital staining. J. Microbiol. Methods 65:623-627.
17. Kondo, T., Y. Ebie, S. Tsuneda, and Y. Inamori. 2007. Detection of Defluvicoccus-related glycogen-accumulating organisms in enhanced biological phosphorus removal processes. Microbes Environ. 22:190-195.
18. Lebaron, P., P. Catala, C. Fajon, F. Joux, J. Baudart, and L. Bernard. 1997. A new sensitive, whole-cell hybridization technique for detection of bacteria involving a biotinylated oligonucleotide probe targeting rRNA and tyramide signal amplification. Appl. Environ. Microbiol. 63:3274-3278.
19. Leclerc, H., and A. Moreau. 2002. Microbiological safety of natural mineral water. FEMS Microbiol. Rev. 26:207-222.
20. Maruyama, F., N. Yamaguchi, T. Kenzaka, K. Tani, and M. Nasu. 2004. Simplified sample preparation using frame spotting method for direct counting of total bacteria by fluorescence microscopy. J. Microbiol. Methods 59:427-431.
21. Nagata, T., T. Someya, T. Konda, et al. 1989. Intercalibration of the acridine orange direct count method of aquatic bacteria. Bull. Jpn. Soc. Microb. Ecol. 4:89-99.
22. Nishimura, M., T. Shimakita, T. Matsuzaki, Y. Tashiro, and K. Kogure. 2008. Automatic counting of FISH-labeled microbes by an LED illuminated detecting apparatus. Fisheries Sci. 74:405-410.
23. Ogawa, M., K. Tani, N. Yamaguchi, and M. Nasu. 2003. Development of multicolour digital image analysis system to enumerate actively respiring bacteria in natural river water. J. Appl. Microbiol. 95:120-128.
24. Pyle, B.H., S.C. Broadaway, and G.A. McFeters. 1999. Sensitive detection of Escherichia coli O157:H7 in food and water by immunomagnetic separation and solid-phase laser cytometry. Appl. Environ. Microbiol. 65:1966-1972.
25. Rockabrand, D., T. Austin, R. Kaiser, and P. Blum. 1999. Bacterial growth state distinguished by single-cell protein profiling: does chlorination kill coliforms in municipal effluent? Appl. Environ. Microbiol. 65:4181-4188.
26. Rodriguez, G.G., D. Phipps, K. Ishiguro, and H.F. Ridgway. 1992. Use of a fluorescent redox probe for direct visualization of actively respiring bacteria. Appl. Environ. Microbiol. 58:1801-1808.
27. Rompre, A., P. Servais, J. Baudart, M.-R. de-Roubin, and P. Laurent. 2002. Detection and enumeration of coliforms in drinking water: current methods and emerging approaches. J. Microbiol. Methods 49: 31-54.
28. Schaule, G., H.-C. Flemming, and H.F. Ridgway. 1993. Use of 5-cyano-2,3-ditolyl tetrazolium chloride for quantifying planktonic and sessile respiring bacteria in drinking water. Appl. Environ. Microbiol. 59:3850-3857.
29. Sheridan, G.E.C., C.I. Masters, J.A. Shallcross, and B.M. Mackey. 1998. Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells. Appl. Environ. Microbiol. 64:1313-1318.
30. Takenaka, T., T. Tashiro, A. Ozaki, H. Takakubo, Y. Yamamoto, T. Maruyama, K. Nagaosa, H. Kimura, and K. Kato. 2007. Planktonic bacterial population dynamics with environmental changes in coastal areas of Suruga Bay. Microbes Environ. 22:257-267.
31. Wallner, G., R. Amann, and W. Beisker. 1993. Optimizing fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes for flow cytometric identification of microorganisms. Cytometry 14:136-143.
32. Wilson, I.G. 1997. Inhibition and facilitation of nucleic acid amplification. Appl. Environ. Microbiol. 63:3741-3751.
33. Yamaguchi, N., and M. Nasu. 1997. Flow cytometric analysis of bacterial respiratory and enzymatic activity in the natural aquatic environment. J. Appl. Microbiol. 83:43-52.
34. Yamaguchi, N., Y. Itoh, M. Masuhara, K. Tani, and M. Nasu. 1999. In situ analysis of community structure in activated sludge with 2hydroxy-3-naphthoic acid-2'-phenylanilide phosphate and Fast Red TR in situ hybridization. Microbes Environ. 14:1-8.