Megabase deletions of gene deserts result in viable mice

Nature

Volumn 431, Issue 7011, 2004, Pages 988-993

  Nóbrega, Marcelo A ^a   Zhu, Yiwen ^a   Plajer Frick, Ingrid ^a   Afzal, Yaena ^a   Rubin, Edward M ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

BIOASSAY; DNA; GENES; GROWTH KINETICS; MORPHOLOGY; PROTEINS;

GENE EXPRESSION; HOMEOSTATIS; MEGABASE DELETIONS;

GENETIC ENGINEERING;

DNA; GENE PRODUCT; PROTEIN MMU19; PROTEIN MMU3; UNCLASSIFIED DRUG;

BIOLOGY;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BASE PAIRING; CELL VIABILITY; CHROMOSOME 19; CHROMOSOME 3; CHROMOSOME DELETION; CONTROLLED STUDY; DNA DETERMINATION; DNA STRUCTURE; FITNESS; GENE DELETION; GENE DESERT; GENE EXPRESSION REGULATION; GENE FUNCTION; GENETIC SCREENING; GENETIC TRAIT; HOMOZYGOSITY; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; REPRODUCTION; TISSUE DISTRIBUTION;

ANIMALS; CHROMOSOME DELETION; CHROMOSOMES, MAMMALIAN; CONSERVED SEQUENCE; FEMALE; GENE EXPRESSION PROFILING; GENES, ESSENTIAL; GENOME; HOMOZYGOTE; HUMANS; LONGEVITY; MALE; MICE; MICE, MUTANT STRAINS; MICE, TRANSGENIC; PHENOTYPE; REPRODUCTION; SEQUENCE DELETION;

ANIMALIA; MAMMALIA; RODENTIA;

EID: 7244259100     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/nature03022     Document Type: Article

References (28)

1. Bentley, D. R. Genomes for medicine. Nature 429, 440-445 (2004).
2. Dunham, A. et al. The DNA sequence and analysis of human chromosome 13. Nature 428, 522-528 (2004).
3. Scherer, S. W. et al. Human chromosome 7: DNA sequence and biology. Science 300, 767-772 (2003).
4. Venter, J. C. et al. The sequence of the human genome. Science 291, 1304-1351 (2001).
5. Jasny, B. R. & Kennedy, D. The human genome. Science 291, 1153 (2001).
6. Gu, Z. et al. Role of duplicate genes in genetic robustness against null mutations. Nature 421, 63-66 (2003).
7. Winzeler, E. A. et al. Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 285, 901-906 (1999).
8. Nelson, C. E., Hersh, B. M. & Carroll, S. B. The regulatory content of intergenic DNA shapes genome architecture. Genome Biol. 5, R25.1-R25.15 (2004).
9. Bejerano, G. et al. Ultraconserved elements in the human genome. Science 304, 1321-1325 (2004).
10. Ramirez-Solis, R., Liu, P. & Bradley, A. Chromosome engineering in mice. Nature 378, 720-724 (1995).
11. Yu, Y. & Bradley, A. Engineering chromosomal rearrangements in mice. Nature Rev. Genet. 2, 780-790 (2001).
12. Zhu, Y. et al. Genomic interval engineering of mice identifies a novel modulator of triglyceride production. Proc. Natl Acad. Sci. USA 97, 1137-1142 (2000).
13. Nobrega, M. A., Ovcharenko, I., Afzal, V. & Rubin, E. M. Scanning human gene deserts for long-range enhancers. Science 302, 413 (2003).
14. Lettice, L. A. et al. A long-range Shh enhancer regulates expression in the developing limb and fin and is associated with preaxial polydactyly. Hum. Mol. Genet. 12, 1725-1735 (2003).
15. Kimura-Yoshida, C. et al. Characterization of the pufferfish Otx2 cis-regulators reveals evolutionarily conserved genetic mechanisms for vertebrate head specification. Development 131, 57-71 (2004).
16. Ovcharenko, I., Loots, G. G. & Stubbs, L. Interpreting mammalian evolution using Fugu genome comparisons. Genomics (in the press).
17. Kothary, R. et al. Inducible expression of an hsp68-lacZ hybrid gene in transgenic mice. Development 105, 707-714 (1989).
18. Boffelli, D., Nobrega, M. A. & Rubin, E. M. Comparative genomics at the vertebrate extremes. Nature Rev. Genet. 5, 456-165 (2004).
19. Zerucha, T. et al. A highly conserved enhancer in the Dlx5/Dlx6 intergenic region is the site of cross-regulatory interactions between Dk genes in the embryonic forebrain. J. Neurosci. 20, 709-721 (2000).
20. Frazer, K. A. et al. Noncoding sequences conserved in a limited number of mammals in the SIM2 interval are frequently functional. Genome Res. 14, 367-372 (2004).
21. Iafrate, A. J. et al. Detection of large-scale variation in the human genome. Nature Genet. 36, 949-951 (2004).
22. Sebat, J. et al. Large-scale copy number polymorphism in the human genome. Science 305, 525-528 (2004).
23. Sauer, B. & Henderson, N. Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase. New Biol. 2, 441-449 (1990).
24. Hogan, B. & Lacy, E. Manipulating the Mouse Embryo: A Laboratory Manual (Cold Spring Harbor Laboratory Press, Plainview, New York, 1994).
25. Rat Genome Sequencing Project Consortium. Genome sequence of the Brown Norway rat yields insights into mammalian evolution. Nature 428, 493-521 (2004).
26. Mouse Genome Sequencing Consortium. Initial sequencing and comparative analysis of the mouse genome. Nature 420, 520-562 (2002).
27. Anderson, J. P. et al. HRC is a direct transcriptional target of MEF2 during cardiac, skeletal, and arterial smooth muscle development in vivo. Mol. Cell. Biol. 24, 3737-3768 (2004).
28. Dodou, E., Xu, S. M. & Black, B. L. mef2c is activated directly by myogenic basic helix-loop-helix proteins during skeletal muscle development in vivo. Mech. Dev. 120, 1021-1032 (2003).