Expression of Trichoderma reesei endo-β-glucanase II in silkworm, Bombyx mori L. by using BmNPV/Bac-to-Bac expression system and its bioactivity assay

Biotechnology Letters

Volumn 32, Issue 1, 2010, Pages 67-72

  Zhou, Lie ^a   Wu, Xiaofeng ^a   Lan, Lipan ^a   Liu, Jianxin ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

BmNPV Bac to Bac; Endoglucanase; Expression system; Feed additive; Silkworm; Trichoderma reesei

Indexed keywords

ANIMALS; CLONING; ENZYME ACTIVITY; PURIFICATION; VIRUSES;

BMNPV/BAC-TO-BAC; ENDOGLUCANASES; EXPRESSION SYSTEM; FEED ADDITIVES; SILKWORM; TRICHODERMA REESEI;

GENE EXPRESSION;

BOMBYX MORI; BOMBYX MORI NPV; FUNGI; HYPOCREA JECORINA; NUCLEOPOLYHEDROVIRUS;

EID: 72249116745     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-009-0129-8     Document Type: Article

References (15)

1. Bailey MJ, Nevalainen KMH (1981) Induction, isolation and testing of stable Trichoderma reesei mutants with improved production of solubilizing cellulase. Enzyme Microb Technol 3: 153-157.
2. Cao C, Wu X, Zhao N, Yao H, Lu X, Tan Y (2006) Development of a rapid and efficient BmNPV baculovirus expression system for application in mulberry silkworm, Bombyx mori. Curr Sci 91(12): 1692-1697.
3. Falkowski P, Scholes RJ, Boyle E, Canadell J, Canfield D, Elser J, Gruber N, Hibbard K, Högberg P, Linder S, Mackenzie FT, Moore B III, Pedersen T, Rosenthal Y, Seitzinger S, Smetacek V, Steffen W (2000) The global carbon cycle: a test of our knowledge of earth as a system. Science 290(5490): 291-296.
4. Higashihashi N, Arai Y, Enjyo T, Horiuchi T, Saeki Y, Sakano K, Sato Y, Takeda K, Takashina S, Takahashi T (1991) High-level expression and characterization of hepatitis B virus surface antigen in silkworm using a baculovirus vector. J Virol Methods 35: 159-167.
5. Himmel ME, Ruth MF, Wyman CE (1999) Cellulase for commodity products from cellulosic biomass. Curr Opin Biotechnol 10(4): 358-364.
6. Ho WKK, Meng ZQ, Lin HR, Poon CT, Leung YK, Yan KT, Dias N, Che APK, Liu J, Zheng WM, Sun Y, Wong AOL (1998) Expression of grass carp growth hormone by baculovirus in silkworm larvae. Biochim Biophys Acta 1381: 331-339.
7. Lee KS, Je YH, Woo SD, Sohn HD, Jin BR (2006) Production of a cellulase in silkworm larvae using a recombinant Bombyx mori nucleopolyhedrovirus lacking the virus-encoded chitinase and cathepsin genes. Biotechnol Lett 28(9): 645-650.
8. Liu T, Zhang YZ, Wu XF (2005) High level expression of functionally active human lactoferrin in silkworm larvae. J Biotechnol 118(3): 246-256.
9. Nevalainen H, Penttilä M (1995) Molecular biology of cellulolytic fungi. In: Kück U (ed) Genetics and biotechnology. The Mycota II, Springer-Verlag, Berlin, pp 303-319.
10. Penttilä ME, André L, Saloheimo M, Lehtovaara P, Knowles JK (1987) Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiae. Yeast 3(3): 175-185.
11. Qiao Y, Mao A, He Y, Liu W, Dong Z (2004) Secreted expression of Trichoderma reesei endo-β-glucanase II gene in pichia pastoris and analysis of enzymic properties. Mycosystema 23(3): 388-396 (in chinese).
12. Saloheimo M, Lehtovaara P, Penttilä M, Teeri TT, Ståhlberg J, Johansson G, Pettersson G, Claeyssens M, Tomme P, Knowles JK (1988) EGIII, a new endoglucanase from Trichoderma reesei: the characterization of both gene and enzyme. Gene 63(1): 11-21.
13. Saloheimo M, Nakari-Setälä T, Tenkanen M, Penttilä M (1997) cDNA cloning of a Trichoderma reesei cellulase and demonstration of endoglucanase activity by expression in yeast. Eur J Biochem 249(2): 584-591.
14. Wu X, Yin Z, Cao C, Ling H, Lu X, Liu J, Cui W (2004) Expression of human VEGF165 in silkworm (Bombyx mori L.) by using a recombinant baculovirus and its bioactivity assay. J Biotechnol 111(3): 253-261.
15. Zhao N, Yao H, Lan L, Cao C, Umashankar ML, Lu X, Wu X, Wang B, Cui W, Cenis JL (2008) Efficient production of canine interferon-alpha in silkworm Bombyx mori by use of a BmNPV/Bac-to-Bac expression system. Appl Microbiol Biotechnol 78: 221-226.