Detection of dermcidin for sweat identification by real-time RT-PCR and ELISA

Forensic Science International

Volumn 194, Issue 1-3, 2010, Pages 80-84

  Sakurada, Koichi ^a   Akutsu, Tomoko ^a   Fukushima, Hisayo ^a   Watanabe, Ken ^a   Yoshino, Mineo ^a  

^a NATIONAL RESEARCH INSTITUTE OF POLICE SCIENCE   (Japan)

Author keywords

Body fluid identification; Dermcidin; ELISA; Real time RT PCR; Sweat

Indexed keywords

DERMCIDIN; MESSENGER RNA;

ADULT; ARTICLE; BODY FLUID; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPERIMENTAL DESIGN; FEMALE; FORENSIC IDENTIFICATION; HUMAN; MALE; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RNA DEGRADATION; SENSITIVITY AND SPECIFICITY; SWEAT; VALIDATION PROCESS;

EID: 72149107334     PISSN: 03790738     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.forsciint.2009.10.015     Document Type: Article

References (26)

1. Takai T., Kooriyama K., Ujiie K., Tsuchiya M., and Hashiyada M. Detection of human hemoglobin with OC-Hemocatch, a commercially available occult blood test. Res. Pract. Forensic Med. 38 (1995) 101-104
2. Juusola J., and Ballantyne J. Messenger RNA profiling: a prototype method to supplant conventional methods for body fluid identification. Forensic Sci. Int. 135 (2003) 85-96
3. Juusola J., and Ballantyne J. Multiplex mRNA profiling for the identification of body fluids. Forensic Sci. Int. 152 (2005) 1-12
4. Nussbaumer C., Gharehbaghi-Schnell E., and Korschineck I. Messenger RNA profiling: a novel method for body fluid identification by Real-Time PCR. Forensic Sci. Int. 157 (2006) 181-186
5. Juusola J., and Ballantyne J. mRNA profiling for body fluid identification by multiplex quantitative RT-PCR. J. Forensic Sci. 52 (2007) 1252-1262
6. Zubakov D., Hanekamp E., Kokshoorn M., van Ijcken W., and Kayser M. Stable RNA markers for identification of blood and saliva stains revealed from whole genome expression analysis of time-wise degraded samples. Int. J. Legal Med. 122 (2008) 135-142
7. Setzer M., Juusola J., and Ballantyne J. Recovery and stability of RNA in vaginal swabs and blood, semen, and saliva stains. J. Forensic Sci. 53 (2008) 296-305
8. Zubakov D., Kokshoorn M., Kloosterman A., and Kayser M. New markers for old stains: stable mRNA markers for blood and saliva identification from up to 16-year-old stains. Int. J. Legal Med. 123 (2009) 71-74
9. Sakurada K., Ikegaya H., Fukushima H., Akutsu T., Watanabe K., and Yoshino M. Evaluation of mRNA-based approach for identification of saliva and semen. Legal Med. 11 (2008) 125-128
10. Nakanishi H. Preliminary test for demonstration of sweat stains by lactic acid. Jpn. J. Forensic Sci. Tech. 10 (2005) 45-48
11. Schittek B., Hipfel R., Sauer B., Bauer J., Kalbacher H., Stevanovic S., Schirle M., Schroeder K., Blin N., Meier F., Rassner G., and Garbe C. Dermcidine: a novel human antibiotic peptide secreted by sweat glands. Nat. Immunol. 2 (2001) 1133-1137
12. Gallo R.L., Ono M., Povsic T., Page C., Eriksson E., Klagsbrun M., and Bernfield M. Syndecans, cell surface heparin sulfate proteoglycans, are induced by a proline-rich antimicrobial peptide from wounds. Proc. Natl. Acad. Sci. U.S.A. 91 (1994) 11035-11039
13. Harder J., Bartels J., Christophers E., and Schroder J.M. A peptide antibiotic from human skin. Nature 387 (1997) 861
14. Rieg S., Garbe C., Sauer B., Kalbacher H., and Schittek B. Dermcidin is constitutively produced by eccrine sweat glands and is not induced in epidermal cells under inflammatory skin conditions. Br. J. Dermatol. 151 (2004) 534-539
15. Porter D., Weremowicz S., Chin K., Seth P., Keshaviah A., Lahti-Domenici J., Bae Y.K., Monitto C.L., Merlos-Suarez A., Chan J., Hulette C.M., Richardson A., Morton C.C., Marks J., Duyao M., Hruban R., Gabrielson E., Gelman R., and Polyak K. A neural survival factor is a candidate oncogene in breast cancer. Proc. Natl. Acad. Sci. U.S.A. 100 (2003) 10931-10936
16. Stewart G.D., Lowrie A.G., Riddick A.C., Fearon K.C., Habib F.K., and Ross J.A. Dermcidin expression confers a survival advantages in prostate cancer cells subjected to oxidative stress of hypoxia. Prostate 67 (2007) 1308-1317
17. Sagawa K., Kimura A., Saito Y., Inoue H., Yasuda S., Nosaka M., and Tsuji T. Production and characterization of a monoclonal antibody for sweat-specific protein and its application for sweat identification. Int. Legal Med. 117 (2003) 90-95
18. Jirka M., and Masopust J. Immunochemical behavior of proteins in human sweat. Biochim. Biophys. Acta 71 (1963) 217-218
19. Herrmann W.P., and Habbig J. Immunological studies on the proteins of human eccrine sweat. Arch. Derm. Res. 255 (1983) 123-127
20. Takizawa H., Kishi K., Yamamoto S., and Iseki S. Human sweat-specific antigen. Proc. Jpn. Acad. 59B (1983) 243-246
21. Marshall T. Analysis of human sweat proteins by two-dimensional electrophoresis and ultrasensitive silver staining. Anal. Biochem. 139 (1984) 506-509
22. Hino D., Miyoshi M., Nakayama H., Moriyoshi H., Morikawa T., Nakaki S., and Itohara K. Identification of sweat stain using E-urocanic acid and L-turosine. Jpn. J. Forensic Sci. Tech. 13 (2008) 17-23
23. Flad T., Bogumil R., Tolson J., Schittek B., Garbe C., Deeg M., Mueller C.A., and Kalbacher H. Detection of dermcidin-derived peptides in sweat by ProteinChip technology. J. Immunol. Methods 270 (2002) 53-62
24. Rieg S., Steffen H., Seeber S., Humeny A., Kalbacher H., Dietz K., Garbe C., and Schittek B. Deficiency of dermcidin-derived antimicrobial peptides in sweat of patients with atopic dermatitis correlates with an impaired innate defense of human skin in vivo. J. Immunol. 174 (2005) 8003-8010
25. Baechle D., Flad T., Cansier A., Steffen H., Schittek B., Tolson J., Herrmann T., Dihazi H., Beck A., Mueller G.A., Mueller M., Stevanovic S., Garbe C., Mueller C.A., and Kalbacher H. Cathepsin D is present in human eccrine sweat and involved in the postsecretory processing of the antimicrobial peptide DCD-1L. J. Biol. Chem. 281 (2006) 5406-5415
26. Stewart G.D., Skipworth R.J., Ross J.A., Fearon K.Ch., and Baracos V.E. The dermcidin gene in cancer: role in cachexia, carcinogenesis and tumour cell survival. Curr. Opin. Clin. Nutr. Metab. Care 11 (2008) 208-213