Mechanical loading regimes affect the anabolic and catabolic activities by chondrocytes encapsulated in PEG hydrogels

Osteoarthritis and Cartilage

Volumn 18, Issue 1, 2010, Pages 126-137

  Nicodemus, G D ^a   Bryant, S J ^a  

^a UCB 450   (United States)

Author keywords

Cartilage; Dynamic loading; Gene expression; Matrix metalloproteinases; Poly(ethylene glycol) hydrogels

Indexed keywords

AGGRECAN; COLLAGEN TYPE 2; COLLAGENASE 3; INTERSTITIAL COLLAGENASE; MACROGOL; STROMELYSIN;

ANIMAL CELL; ARTICLE; CARTILAGE CELL; CELL ENCAPSULATION; CHONDROGENESIS; COMPRESSION; CONTROLLED STUDY; COW; DOWN REGULATION; EXTRACELLULAR MATRIX; GENE EXPRESSION; MECHANICAL STIMULATION; MECHANICAL STRESS; METABOLISM; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; TISSUE ENGINEERING; TISSUE REGENERATION; UPREGULATION;

AGGRECANS; ANIMALS; CARTILAGE, ARTICULAR; CATTLE; CHONDROCYTES; COLLAGEN; DOWN-REGULATION; EXTRACELLULAR MATRIX; GENE EXPRESSION; HYDROGELS; MATRIX METALLOPROTEINASES; POLYETHYLENE GLYCOLS; POLYMERASE CHAIN REACTION; RNA; STRESS, MECHANICAL; TIME FACTORS; TISSUE ENGINEERING; UP-REGULATION; WEIGHT-BEARING;

EID: 72149105318     PISSN: 10634584     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.joca.2009.08.005     Document Type: Article

References (44)

1. Guilak F., Butler D.L., and Goldstein S.A. Functional tissue engineering - the role of biomechanics in articular cartilage repair. Clin Orthop Relat Res (2001) S295-S305
2. Hung C.T., Mauck R.L., Wang C.C.B., Lima E.G., and Ateshian G.A. A paradigm for functional tissue engineering of articular cartilage via applied physiologic deformational loading. Ann Biomed Eng 32 (2004) 35-49
3. Mauck R.L., Byers B.A., Yuan X., and Tuan R.S. Regulation of cartilaginous ECM gene transcription by chondrocytes and MSCs in 3D culture in response to dynamic loading. Biomech Model Mechanobiol 6 (2007) 113-125
4. Chung C., Erickson I.E., Mauck R.L., and Burdick J.A. Differential behavior of auricular and articular chondrocytes in hyaluronic acid hydrogels. Tissue Eng Part A 14 (2008) 1121-1131
5. Clark I.M., and Murphy G. Matrix proteinases. In: Seibel M.J., Robins S.P., and Bilezikian J.P. (Eds). Dynamics of Bone and Cartilage Metabolism (2006), Elsevier, Burlington 181-198
6. Manicourt D.H., Devogelaer J.P., and Thonar E.J. Products of cartilage metabolism. In: Seibel M.J., Robins S.P., and Bilezikian J.P. (Eds). Dynamics of Bone and Cartilage Metabolism (2006), Elsevier, Burlington 421-449
7. Bau B., Gebhard P.M., Haag J., Knorr T., Bartnik E., and Aigner T. Relative messenger RNA expression profiling of collagenases and aggrecanases in human articular chondrocytes in vivo and in vitro. Arthritis Rheum 46 (2002) 2648-2657
8. Kevorkian L., Young D.A., Darrah C., Donell S.T., Shepstone L., Porter S., et al. Expression profiling of metalloproteinases and their inhibitors in cartilage. Arthritis Rheum 50 (2004) 131-141
9. De Croos J.N.A., Dhaliwal S.S., Grynpas M.D., Pilliar R.M., and Kandel R.A. Cyclic compressive mechanical stimulation induces sequential catabolic and anabolic gene changes in chondrocytes resulting in increased extracellular matrix accumulation. Matrix Biol 25 (2006) 323-331
10. Bryant S.J., and Anseth K.S. Hydrogel properties influence ECM production by chondrocytes photoencapsulated in poly(ethylene glycol) hydrogels. J Biomed Mater Res 59 (2002) 63-72
11. Bryant S.J., Bender R.J., Durand K.L., and Anseth K.S. Encapsulating chondrocytes in degrading PEG hydrogels with high modulus: engineering gel structural changes to facilitate cartilaginous tissue production. Biotechnol Bioeng 86 (2004) 747-755
12. Elisseeff J. Injectable cartilage tissue engineering. Expert Opin Biol Ther 4 (2004) 1849-1859
13. Nicodemus G.D., and Bryant S.J. The role of hydrogel structure and dynamic loading on chondrocyte gene expression and matrix formation. J Biomech 41 (2008) 1528-1536
14. Villanueva I., Hauschulz D.S., Mejic D., and Bryant S.J. Static and dynamic compressive strains influence nitric oxide production and chondrocyte bioactivity when encapsulated in PEG hydrogels of different crosslinking densities. Osteoarthritis Cartilage 16 (2008) 909-918
15. Chowdhury T.T., Bader D.L., Shelton J.C., and Lee D.A. Temporal regulation of chondrocyte metabolism in agarose constructs subjected to dynamic compression. Arch Biochem Biophys 417 (2003) 105-111
16. Kelly T.A.N., Ng K.W., Wang C.C.B., Ateshian G.A., and Hung C.T. Spatial and temporal development of chondrocyte-seeded agarose constructs in free-swelling and dynamically loaded cultures. J Biomech 39 (2006) 1489-1497
17. Kisiday J.D., Jin M.S., DiMicco M.A., Kurz B., and Grodzinsky A.J. Effects of dynamic compressive loading on chondrocyte biosynthesis in self-assembling peptide scaffolds. J Biomech 37 (2004) 595-604
18. Mauck R.L., Seyhan S.L., Ateshian G.A., and Hung C.T. Influence of seeding density and dynamic deformational loading on the developing structure/function relationships of chondrocyte-seeded agarose hydrogels. Ann Biomed Eng 30 (2002) 1046-1056
19. Mauck R.L., Soltz M.A., Wang C.C.B., Wong D.D., Chao P.H.G., Valhmu W.B., et al. Functional tissue engineering of articular cartilage through dynamic loading of chondrocyte-seeded agarose gels. ASME J Biomech Eng 122 (2000) 252-260
20. Waldman S.D., Couto D.C., Grynpas M.D., Pilliar R.M., and Kandel R.A. A single application of cyclic loading can accelerate matrix deposition and enhance the properties of tissue-engineered cartilage. Osteoarthritis Cartilage 14 (2006) 323-330
21. Lima E.G., Bian L., Ng K.W., Mauck R.L., Ba B., Tuan R.S., et al. The beneficial effect of delayed compressive loading on tissue-engineered cartilage constructs cultured with TGF-beta 3. Osteoarthritis Cartilage 15 (2007) 1025-1033
22. Anseth K.S., Metters A.T., Bryant S.J., Martens P.J., Elisseeff J.H., and Bowman C.N. In situ forming degradable networks and their application in tissue engineering and drug delivery. J Control Release 78 (2002) 199-209
23. Bryant S.J., and Anseth K.S. Controlling the spatial distribution of ECM components in degradable PEG hydrogels for tissue engineering cartilage. J Biomed Mater Res A 64A (2003) 70-79
24. Bryant S.J., Durand K.L., and Anseth K.S. Manipulations in hydrogel chemistry control photoencapsulated chondrocyte behavior and their extracellular matrix production. J Biomed Mater Res A 67A (2003) 1430-1436
25. Sawhney A.S., Pathak C.P., and Hubbell J.A. Bioerodible hydrogels based on photopolymerized poly(ethylene glycol)-co-poly(alpha-hydroxy acid) diacrylate macromers. Macromolecules 26 (1993) 581-587
26. Bryant S.J., Nuttelman C.R., and Anseth K.S. Cytocompatibility of UV and visible light photoinitiating systems on cultured NIH/3T3 fibroblasts in vitro. J Biomater Sci Polym Ed 11 (2000) 439-457
27. Chomczynski P. A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques 15 (1993) 532
28. Pfaffl M.W. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29 (2001)
29. Farndale R.W., Buttle D.J., and Barrett A.J. Improved quantitation and discrimination of sulfated glycosaminoglycans by use of dimethylmethylene blue. Biochim Biophys Acta 883 (1986) 173-177
30. Davisson T., Kunig S., Chen A., Sah R., and Ratcliffe A. Static and dynamic compression modulate matrix metabolism in tissue engineered cartilage. J Orthop Res 20 (2002) 842-848
31. Kisiday J.D., Lee J.H., Siparsky P.N., Frisbie D.D., Flannery C.R., Sandy J.D., et al. Catabolic responses of chondrocyte-seeded peptide hydrogel to dynamic compression. Ann Biomed Eng 37 (2009) 1368-1375
32. Blain E.J. Mechanical regulation of matrix metalloproteinases. Front Biosci 12 (2007) 507-527
33. Guilak F., Alexopoulos L.G., Upton M.L., Youn I., Choi J.B., Cao L., et al. The pericellular matrix as a transducer of biomechanical and biochemical signals in articular cartilage. Skeletal Development and Remodeling in Health, Disease, and Aging 1068 (2006) 498-512
34. Poole C.A. Articular cartilage chondrons: form, function and failure. J Anat 191 (1997) 1-13
35. Bryant S.J., Chowdhury T.T., Lee D.A., Bader D.L., and Anseth K.S. Crosslinking density influences chondrocyte metabolism in dynamically loaded photocrosslinked poly(ethylene glycol) hydrogels. Ann Biomed Eng 32 (2004) 407-417
36. Bryant S.J., Anseth K.S., Lee D.A., and Bader D.L. Crosslinking density influences the morphology of chondrocytes photoencapsulated in PEG hydrogels during the application of compressive strain. J Orthop Res 22 (2004) 1143-1149
37. Grodzinsky A.J., Levenston M.E., Jin M., and Frank E.H. Cartilage tissue remodeling in response to mechanical forces. Annu Rev Biomed Eng 2 (2000) 691-713
38. Quinn T.M., Grodzinsky A.J., Buschmann M.D., Kim Y.J., and Hunziker E.B. Mechanical compression alters proteoglycan deposition and matrix deformation around individual cells in cartilage explants. J Cell Sci 111 (1998) 573-583
39. Sah R.L.Y., Kim Y.J., Doong J.Y.H., Grodzinsky A.J., Plaas A.H.K., and Sandy J.D. Biosynthetic response of cartilage explants to dynamic compression. J Orthop Res 7 (1989) 619-636
40. Pufe T., Lemke A., Kurz B., Petersen W., Tillmann B., Grodzinsky A.J., et al. Mechanical overload induces VEGF in cartilage discs via hypoxia-inducible factor. Am J Pathol 164 (2004) 185-192
41. Buschmann M.D., Kim Y.J., Wong M., Frank E., Hunziker E.B., and Grodzinsky A.J. Stimulation of aggrecan synthesis in cartilage explants by cyclic loading is localized to regions of high interstitial fluid flow. Arch Biochem Biophys 366 (1999) 1-7
42. Evans R.C., and Quinn T.M. Dynamic compression augments interstitial transport of a glucose-like solute in articular cartilage. Biophys J 91 (2006) 1541-1547
43. Garcia A.M., Frank E.H., Grimshaw P.E., and Grodzinsky A.J. Contributions of fluid convection and electrical migration to transport in cartilage: relevance to loading. Arch Biochem Biophys 333 (1996) 317-325
44. Hardingham T. Proteoglycans and glycosaminoglycans. In: Seibel M.J., Robins S.P., and Bilezikian J.P. (Eds). Dynamics of Bone and Cartilage Metabolism (2006), Elsevier, Burlington 85