Preparation of transcriptionally active nuclear extracts from mammalian tissues

BioTechniques

Volumn 21, Issue 1, 1996, Pages 48-52

  Stuempfle, Kristin J ^a   Koptides, Michael ^a   Karinch, Anne M ^a   Floras, Joanna ^a  

^a PENN STATE COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LUNG SURFACTANT;

ANIMAL TISSUE; ARTICLE; LUNG FUNCTION; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION; REPORTER GENE; TRANSCRIPTION INITIATION;

EID: 0029938951     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96211bm09     Document Type: Article

References (6)

1. Buratowski, S. 1994. The basics of basal transcription by RNA polymerase II. Cell 77:1-3.
2. Floros, J., D.S. Phelps, S. Kourembanas and H.W. Taeusch. 1986. Primary translation products, biosynthesis, and tissue specificity of the major surfactant protein in rat. J. Biol. Chem. 267:828-831.
3. Gorski, K., M. Carneiro and U. Schibler. 1986. Tissue-specific in vitro transcription from the mouse albumin promoter. Cell 47:767-776.
4. Kouretas, D., A.M. Karinen, A. Rishi, K. Melchers and J. Floras. 1993. Conservation analysis of rat and human SP-A gene identifies 5′ flanking sequences of rat SP-A that bind rat lung nuclear proteins. Exp. Lung Res. 19:485-503.
5. Sawadogo, M. and R.G. Boeder. 1985 Factors involved in specific transcription by human RNA polymerase II: analysis by a rapid and quantitative in vitro assay. Proc. Natl. Acad. Sci. USA 82:4394-4398.
6. Wang, J., P. Souza, M. Kuliszewski, A.K. Tanswell and M. Post. 1994. Expression of surfactant proteins in embryonic rat lung. Am. J. Respir. Cell Mol. Biol. 10:222-229.