Validation and application of a liquid chromatography-tandem mass spectrometric method for the simultaneous determination of testosterone and dihydrotestosterone in rat prostatic tissue using a 96-well format

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 877, Issue 29, 2009, Pages 3515-3521

  O'Brien, Zhihong ^a   Post, Noah ^a   Brown, Michael ^a   Madan, Ajay ^a   Coon, Timothy ^a   Luo, Rosa ^a   Kohout, Trudy A ^a  

^a NEUROCRINE BIOSCIENCES INC   (United States)

Author keywords

96 well plate; Dihydrotestosterone; Liquid chromatography tandem mass spectrometry; Rat prostatic tissue; Testosterone; Validation

Indexed keywords

96-WELL FORMAT; 96-WELL PLATE; CALIBRATION CURVES; CORRELATION COEFFICIENT; DERIVATIZATION OF; DIHYDROTESTOSTERONE; LC-MS/MS; LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY; LOWER LIMIT OF DETECTIONS; LOWER LIMITS; MATRIX; METHOD VALIDATIONS; PROSTATIC TISSUES; SAMPLE PREPARATION PROCEDURE; SERUM CONCENTRATION; SIMULTANEOUS DETECTION; SIMULTANEOUS DETERMINATIONS; STANDARD SOLUTIONS; TANDEM MASS SPECTROMETRIC; TESTOSTERONE; VALIDATED METHODS; VALIDATION RESULTS;

CALIBRATION; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; HISTOLOGY; LIQUIDS; MASS SPECTROMETERS; MASS SPECTROMETRY; TISSUE;

HIGH PRESSURE LIQUID CHROMATOGRAPHY;

ANDROSTANOLONE; TESTOSTERONE;

ACCURACY; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CALIBRATION; DERIVATIZATION; EXTRACTION; HORMONE DETERMINATION; LIQUID CHROMATOGRAPHY; MALE; NONHUMAN; PRIORITY JOURNAL; PROSTATE; RAT; TANDEM MASS SPECTROMETRY; VALIDATION STUDY;

ANIMALS; CHROMATOGRAPHY, LIQUID; DIHYDROTESTOSTERONE; MALE; PROSTATE; RATS; RATS, SPRAGUE-DAWLEY; REPRODUCIBILITY OF RESULTS; TANDEM MASS SPECTROMETRY; TESTOSTERONE;

RATTUS;

EID: 72049113056     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2009.08.053     Document Type: Article

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