Inexpensive synthetic-based matrix for both conventional and rapid purification of protein A- and tandem affinity purification-tagged proteins

Analytical Biochemistry

Volumn 397, Issue 2, 2010, Pages 241-243

  Sawin, Kenneth E ^a   Bicho, Claudia C ^a   Snaith, Hilary A ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

PURIFICATION;

DYNABEADS; EUKARYOTIC CELLS; IMMUNOGLOBULIN G; PROTEIN A; RAPID PURIFICATION; REDUCED COST; TAGGED PROTEINS; TANDEM AFFINITY PURIFICATION;

PROTEINS;

EPOXY RESIN; IMMUNOGLOBULIN G; NANOBEAD; PROTEIN A; SEPHAROSE;

ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; COST BENEFIT ANALYSIS; GEL; INTERMETHOD COMPARISON; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CLEAVAGE; PROTEIN PROCESSING; PROTEIN PURIFICATION; TANDEM AFFINITY PURIFICATION;

EUKARYOTA;

EID: 72049092013     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.09.045     Document Type: Article

References (12)

1. Rigaut G., Shevchenko A., Rutz B., Wilm M., Mann M., and Seraphin B. A generic protein purification method for protein complex characterization and proteome exploration. Nat. Biotechnol. 17 (1999) 1030-1032
2. Puig O., Caspary F., Rigaut G., Rutz B., Bouveret E., Bragado-Nilsson E., Wilm M., and Seraphin B. The tandem affinity purification (TAP) method: a general procedure of protein complex purification. Methods 24 (2001) 218-229
3. Nilsson B., Moks T., Jansson B., Abrahmsen L., Elmblad A., Holmgren E., Henrichson C., Jones T.A., and Uhlen M. A synthetic IgG-binding domain based on staphylococcal protein A. Protein Eng. 1 (1987) 107-113
4. Snaith H.A., Samejima I., and Sawin K.E. Multistep and multimode cortical anchoring of tea1p at cell tips in fission yeast. EMBO J. 24 (2005) 3690-3699
5. Oeffinger M., Wei K.E., Rogers R., DeGrasse J.A., Chait B.T., Aitchison J.D., and Rout M.P. Comprehensive analysis of diverse ribonucleoprotein complexes. Nat. Methods 4 (2007) 951-956
6. Smith P.K., Krohn R.I., Hermanson G.T., Mallia A.K., Gartner F.H., Provenzano M.D., Fujimoto E.K., Goeke N.M., Olson B.J., and Klenk D.C. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150 (1985) 76-85
7. Stich T.M. Determination of protein covalently bound to agarose supports using bicinchoninic acid. Anal. Biochem. 191 (1990) 343-346
8. Sawin K.E., Lourenco P.C., and Snaith H.A. Microtubule nucleation at non-spindle pole body microtubule-organizing centers requires fission yeast centrosomin-related protein mod20p. Curr. Biol. 14 (2004) 763-775
9. Venkatram S., Tasto J.J., Feoktistova A., Jennings J.L., Link A.J., and Gould K.L. Identification and characterization of two novel proteins affecting fission yeast γ-tubulin complex function. Mol. Biol. Cell 15 (2004) 2287-2301
10. Zachariae W., Shin T.H., Galova M., Obermaier B., and Nasmyth K. Identification of subunits of the anaphase-promoting complex of Saccharomyces cerevisiae. Science 274 (1996) 1201-1204
11. Yoon H.J., Feoktistova A., Wolfe B.A., Jennings J.L., Link A.J., and Gould K.L. Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes. Curr. Biol. 12 (2002) 2048-2054
12. Tackett A.J., DeGrasse J.A., Sekedat M.D., Oeffinger M., Rout M.P., and Chait B.T. I-DIRT, a general method for distinguishing between specific and nonspecific protein interactions. J. Proteome Res. 4 (2005) 1752-1756