Generation of platform human embryonic stem cell lines that allow efficient targeting at a predetermined genomic location

Stem Cells and Development

Volumn 18, Issue 10, 2009, Pages 1459-1471

  Liu, Ying ^a   Thyagarajan, Bhaskar ^a   Lakshmipathy, Uma ^a   Xue, Haipeng ^a   Lieu, Pauline ^a   Fontes, Andrew ^a   MacArthur, Chad C ^a   Scheyhing, Kelly ^a   Rao, Mahendra S ^a   Chesnut, Jonathan D ^a  

^a Life Technologies   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

INTEGRASE; INTEGRASE PHI C31; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL LINE; CELL LINEAGE; CELL REGENERATION; CHROMOSOMAL LOCALIZATION; CONTROLLED STUDY; EMBRYONIC STEM CELL; GENE CONTROL; GENE EXPRESSION; GENE MAPPING; GENE TARGETING; GENETIC TRANSCRIPTION; GENOME; GENOMICS; HUMAN; HUMAN CELL; INTEGRATION; NERVE CELL; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; REPORTER GENE; SITE DIRECTED MUTAGENESIS; TRANSGENE; WILD TYPE;

ANIMALS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINE; CELL LINEAGE; CELL PROLIFERATION; CHROMOSOMES, HUMAN, PAIR 13; CLONE CELLS; EMBRYONIC STEM CELLS; GENE SILENCING; GENETIC LOCI; GENOME, HUMAN; GREEN FLUORESCENT PROTEINS; HUMANS; MICE; MUTAGENESIS, SITE-DIRECTED; NEURONS; OCTAMER TRANSCRIPTION FACTOR-3; PLURIPOTENT STEM CELLS; PROMOTER REGIONS, GENETIC; REPRODUCIBILITY OF RESULTS;

EID: 71949108136     PISSN: 15473287     EISSN: None     Source Type: Journal    
DOI: 10.1089/scd.2009.0047     Document Type: Article

References (38)

1. Smith JR, S Maguire, LA Davis, M Alexander, F Yang, S Chandran, C ffrench-Constant and RA Pedersen. (2008). Robust, persistent transgene expression in human embryonic stem cells is achieved with AAVS1-targeted integration. Stem Cells 26:496-504.
2. Tan SM and P Droge. (2005). Comparative analysis of sequencespecifi c DNA recombination systems in human embryonic stem cells. Stem Cells 23:868-873.
3. Tan SM, ST Wang, H Hentze and P Droge. (2007). A UTF1-based selection system for stable homogeneously pluripotent human embryonic stem cell cultures. Nucleic Acids Res 35:e118.
4. Wilber A, JL Linehan, X Tian, PS Woll, JK Morris, LR Belur, RS McIvor and DS Kaufman. (2007). Effi cient and stable transgene expression in human embryonic stem cells using transposonmediated gene transfer. Stem Cells 25:2919-2927.
5. Zwaka TP and JA Thomson. (2003). Homologous recombination in human embryonic stem cells. Nat Biotechnol 21:319-321.
6. Zhou BY, Z Ye, G Chen, ZP Gao, YA Zhang and L Cheng. (2007). Inducible and reversible transgene expression in human stem cells after effi cient and stable gene transfer. Stem Cells 25:779-789.
7. Kim S, GJ Kim, H Miyoshi, SH Moon, SE Ahn, JH Lee, HJ Lee, KY Cha and HM Chung. (2007). Effi ciency of the elongation factor- 1alpha promoter in mammalian embryonic stem cells using lentiviral gene delivery systems. Stem Cells Dev 16:537-545.
8. Gropp M, P Itsykson, O Singer, T Ben-Hur, E Reinhartz, E Galun and BE Reubinoff. (2003). Stable genetic modifi cation of human embryonic stem cells by lentiviral vectors. Mol Ther 7:281-287.
9. Ma Y, A Ramezani, R Lewis, RG Hawley and JA Thomson. (2003). High-level sustained transgene expression in human embryonic stem cells using lentiviral vectors. Stem Cells 21:111-117.
10. Muegge K, S Xi and T Geiman. (2008). The see-saw of differentiation: Tipping the chromatin balance. Mol Interv 8:15-18, 12.
11. Rasmussen TP. (2003). Embryonic stem cell differentiation: A chromatin perspective. Reprod Biol Endocrinol 1:100.
12. Golob JL, SL Paige, V Muskheli, L Pabon and CE Murry. (2008). Chromatin remodeling during mouse and human embryonic stem cell differentiation. Dev Dyn 237:1389-1398.
13. Minoguchi S and H Iba. (2008). Instability of retroviral DNA methylation in embryonic stem cells. Stem Cells 26:1166-1173.
14. Hope IA, J Stevens, A Garner, J Hayes, DL Cheo, MA Brasch and M Vidal. (2004). Feasibility of genome-scale construction of promoter::reporter gene fusions for expression in Caenorhabditis elegans using a multisite gateway recombination system. Genome Res 14:2070-2075.
15. Kwan KM, E Fujimoto, C Grabher, BD Mangum, ME Hardy, DS Campbell, JM Parant, HJ Yost, JP Kanki and CB Chien. (2007). The Tol2kit: A multisite gateway-based construction kit for Tol2 transposon transgenesis constructs. Dev Dyn 236:3088-3099.
16. Sasaki Y, T Sone, K Yahata, H Kishine, J Hotta, JD Chesnut, T Honda and F Imamoto. (2008). Multi-gene gateway clone design for expression of multiple heterologous genes in living cells: Eukaryotic clones containing two and three ORF multigene cassettes expressed from a single promoter. J Biotechnol 136:103-112.
17. Sasaki Y, T Sone, S Yoshida, K Yahata, J Hotta, JD Chesnut, T Honda and F Imamoto. (2004). Evidence for high specifi city and effi ciency of multiple recombination signals in mixed DNA cloning by the Multisite Gateway system. J Biotechnol 107:233-243.
18. Yahata K, H Kishine, T Sone, Y Sasaki, J Hotta, JD Chesnut, M Okabe and F Imamoto. (2005). Multi-gene gateway clone design for expression of multiple heterologous genes in living cells: Conditional gene expression at near physiological levels. J Biotechnol 118:123-134.
19. Groth AC, EC Olivares, B Thyagarajan and MP Calos. (2000). A phage integrase directs effi cient site-specifi c integration in human cells. Proc Natl Acad Sci USA 97:5995-6000.
20. Thyagarajan B, EC Olivares, RP Hollis, DS Ginsburg and MP Calos. (2001). Site-specifi c genomic integration in mammalian cells mediated by phage phiC31 integrase. Mol Cell Biol 21:3926-3934.
21. Chalberg TW, HL Genise, D Vollrath and MP Calos. (2005). phiC31 integrase confers genomic integration and long-term transgene expression in rat retina. Invest Ophthalmol Vis Sci 46:2140-2146.
22. Chalberg TW, JL Portlock, EC Olivares, B Thyagarajan, PJ Kirby, RT Hillman, J Hoelters and MP Calos. (2006). Integration specifi city of phage phiC31 integrase in the human genome. J Mol Biol 357:28-48.
23. Thyagarajan B, Y Liu, S Shin, U Lakshmipathy, K Scheyhing, H Xue, C Ellerstrom, R Strehl, J Hyllner, MS Rao and JD Chesnut. (2008). Creation of engineered human embryonic stem cell lines using phiC31 integrase. Stem Cells 26:119-126.
24. Wohlgemuth JG, SH Kang, GH Bulboaca, KA Nawotka and MP Calos. (1996). Long-term gene expression from autonomously replicating vectors in mammalian cells. Gene Ther 3:503-512.
25. Olivares EC, RP Hollis and MP Calos. (2001). Phage R4 integrase mediates site-specifi c integration in human cells. Gene 278:167-176.
26. Zeng X, J Chen, Y Liu, Y Luo, TC Schulz, AJ Robins, MS Rao and WJ Freed. (2004). BG01V: A variant human embryonic stem cell line which exhibits rapid growth after passaging and reliable dopaminergic differentiation. Restor Neurol Neurosci 22:421-428.
27. Zeng X, T Miura, Y Luo, B Bhattacharya, B Condie, J Chen, I Ginis, I Lyons, J Mejido, RK Puri, MS Rao and WJ Freed. (2004). Properties of pluripotent human embryonic stem cells BG01 and BG02. Stem Cells 22:292-312. (Pubitemid 38736614)
28. Benzing C, M Segschneider, A Leinhaas, J Itskovitz-Eldor and O Brustle. (2006). Neural conversion of human embryonic stem cell colonies in the presence of fi broblast growth factor-2. Neuroreport 17:1675-1681.
29. Liu Y, SS Han, Y Wu, TM Tuohy, H Xue, J Cai, SA Back, LS Sherman, I Fischer and MS Rao. (2004). CD44 expression identifi es astrocyte-restricted precursor cells. Dev Biol 276:31-46.
30. Mulsant P, A Gatignol, M Dalens and G Tiraby. (1988). Phleomycin resistance as a dominant selectable marker in CHO cells. Somat Cell Mol Genet 14:243-252.
31. Rondeau JM, C Cagnon, D Moras and JM Masson. (1989). Crystallization and preliminary X-ray data of a phleomycinthe binding protein from Streptoalloteichus hindustanus. J Mol Biol 207:645-646.
32. Maitra A, DE Arking, N Shivapurkar, M Ikeda, V Stastny, K Kassauei, G Sui, DJ Cutler, Y Liu, SN Brimble, K Noaksson, J Hyllner, TC Schulz, X Zeng, WJ Freed, J Crook, S Abraham, A Colman, P Sartipy, S Matsui, M Carpenter, AF Gazdar, M Rao and A Chakravarti. (2005). Genomic alterations in cultured human embryonic stem cells. Nat Genet 37:1099-1103. (Pubitemid 41486662)
33. Ehrhardt A, JA Engler, H Xu, AM Cherry and MA Kay. (2006). Molecular analysis of chromosomal rearrangements in mammalian cells after phiC31-mediated integration. Hum Gene Ther 17:1077-1094.
34. Liu J, I Jeppesen, K Nielsen and TG Jensen. (2006). Phi c31 integrase induces chromosomal aberrations in primary human fi broblasts. Gene Ther 13:1188-1190.
35. Sone T, K Yahata, Y Sasaki, J Hotta, H Kishine, JD Chesnut and F Imamoto. (2008). Multi-gene gateway clone design for expression of multiple heterologous genes in living cells: Modular construction of multiple cDNA expression elements using recombinant cloning. J Biotechnol 136:113-121.
36. Costa M, M Dottori, E Ng, SM Hawes, K Sourris, P Jamshidi, MF Pera, AG Elefanty and EG Stanley. (2005). The hESC line Envy expresses high levels of GFP in all differentiated progeny. Nat Methods 2:259-260.
37. Irion S, H Luche, P Gadue, HJ Fehling, M Kennedy and G Keller. (2007). Identifi cation and targeting of the ROSA26 locus in human embryonic stem cells. Nat Biotechnol 25:1477-1482.
38. Calos MP. (2006). The phiC31 integrase system for gene therapy. Curr Gene Ther 6:633-645.