φc31 integrase induces chromosomal aberrations in primary human fibroblasts

Gene Therapy

Volumn 13, Issue 15, 2006, Pages 1188-1190

  Liu, J ^a   Jeppesen, I ^a   Nielsen, K ^a   Jensen, T G ^a  

^a JOHN F KENNEDY INSTITUTE   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

INTEGRASE;

ARTICLE; CHROMOSOME ABERRATION; CHROMOSOME TRANSLOCATION; CONTROLLED STUDY; CYTOGENETICS; FIBROBLAST; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MUTAGENESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ATTACHMENT SITES, MICROBIOLOGICAL; BACILLUS PHAGES; CELLS, CULTURED; CHROMOSOME ABERRATIONS; FIBROBLASTS; GENE THERAPY; HUMANS; INTEGRASES; KARYOTYPING; POLYMERASE CHAIN REACTION; TRANSFECTION;

EID: 33746124221     PISSN: 09697128     EISSN: 14765462     Source Type: Journal    
DOI: 10.1038/sj.gt.3302789     Document Type: Article

References (15)

1. Thorpe HM, Smith MC. In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the resolvase/invertase family. Proc Natl Acad Sci USA 1998; 95: 5505-5510.
2. Thyagarajan B, Olivares EC, Hollis RP, Ginsburg DS, Calos MP. Site-specific genomic integration in mammalian cells mediated by phage phiC31 integrase. Mol Cell Biol 2001; 21: 3926-3934.
3. Chalberg TW, Portlock JL, Olivares EC, Thyagarajan B, Kirby PJ, Hillman RT et al. Integration specificity of phage varphiC31 integrase in the human genome. J Mol Biol 2005; 357: 28-48.
4. Ginsburg DS, Calos MP. Site-specific integration with phiC31 integrase for prolonged expression of therapeutic genes. Adv Genet 2005; 54: 179-187.
5. Olivares EC, Hollis RP, Chalberg TW, Meuse L, Kay MA, Calos MP. Site-specific genomic integration produces therapeutic Factor IX levels in mice. Nat Biotechnol 2002; 20: 1124-1128.
6. Ortiz-Urda S, Thyagarajan B, Keene DR, Lin Q, Fang M, Calos MP et al. Stable nonviral genetic correction of inherited human skin disease. Nat Med 2002; 8: 1166-1170.
7. Ortiz-Urda S, Thyagarajan B, Keene DR, Lin Q, Calos MP, Khavari PA. PhiC31 integrase-mediated nonviral genetic correction of junctional epidermolysis bullosa. Hum Gene Ther 2003; 14: 923-928.
8. Held PK, Olivares EC, Aguilar CP, Finegold M, Calos MP, Grompe M. In vivo correction of murine hereditary tyrosinemia type I by phiC31 integrase-mediated gene delivery. Mol Ther 2005; 11: 399-408.
9. Ehrhardt A, Xu H, Huang Z, Engler JA, Kay MA. A direct comparison of two nonviral gene therapy vectors for somatic integration: In vivo evaluation of the bacteriophage integrase phiC31 and the Sleeping Beauty transposase. Mol Ther 2005; 11: 695-706.
10. Mitelman F. ISCN: An International System for Human Cytogenetics Nomenclature. S Karger: Basel, Switzerland, 1995.
11. Loonstra A, Vooijs M, Beverloo HB, Allak BA, van Drunen E, Kanaar R et al. Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells. Proc Natl Acad Sci USA 2001; 98: 9209-9214.
12. Belteki G, Gertsenstein M, Ow DW, Nagy A. Site-specific cassette exchange and germline transmission with mouse ES cells expressing phiC31 integrase. Nat Biotechnol 2003; 21: 321-324.
13. Hollis RP, Stoll SM, Sclimenti CR, Lin J, Chen-Tsai Y, Calos MP. Phage integrases for the construction and manipulation of transgenic mammals. Reprod Biol Endocrinol 2003; 1: 79.
14. Groth AC, Fish M, Nusse R, Calos MP. Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31. Genetics 2004; 166: 1775-1782.
15. McKinlay Gardner RJ, Sutherland GR. Chromosome Abnormalities and Genetic Counseling. Oxford University Press: Oxford, 2004, pp 142-162.