Simultaneous double labelling of routinely processed paraffin tissue sections using combined immunoperoxidase, immunofluorescence, and digital image editing

Research in Veterinary Science

Volumn 88, Issue 1, 2010, Pages 122-126

  Ressel, L ^a   Poli, A ^a  

^a UNIVERSITY OF PISA   (Italy)

Author keywords

Double immunohistological labelling; Image editing; Immunofluorescence and immunoperoxidase

Indexed keywords

CALBINDIN; CELL ANTIGEN; CYTOKERATIN ANTIBODY; PARAFFIN; PEROXIDASE; PROTEIN ANTIBODY; VIMENTIN;

ANIMAL CELL; ANIMAL TISSUE; ANTIGEN DETECTION; ARTICLE; CEREBELLUM; CONTROLLED STUDY; DIGITAL IMAGING; DOG; IMAGE PROCESSING; IMMUNOFLUORESCENCE; IMMUNOPEROXIDASE STAINING; NONHUMAN; PLEURA MESOTHELIOMA; PROTEIN LOCALIZATION; SKIN; TISSUE PREPARATION; TISSUE SECTION; TISSUE STRUCTURE;

ANIMALS; ANTIBODIES; CAT DISEASES; CATS; CEREBELLUM; DOG DISEASES; DOGS; FLUORESCENT ANTIBODY TECHNIQUE; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; IMAGE PROCESSING, COMPUTER-ASSISTED; IMMUNOENZYME TECHNIQUES; MESOTHELIOMA; PARAFFIN EMBEDDING; SKIN;

FELIDAE;

EID: 71649096917     PISSN: 00345288     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.rvsc.2009.07.003     Document Type: Article

References (19)

1. Bacci B., Morandi F., De Meo M., and Marcato P.S. Ten cases of feline mesothelioma: an immunohistochemical and ultrastructural study. Journal of Comparative Pathology 134 (2006) 347-354
2. Bombardi C., Grandis A., Chiocchetti R., Bortolami R., Johansson H., and Lucchi M.L. Immunohistochemical localization of alpha(1a)-adrenoreceptors in muscle spindles of rabbit masseter muscle. Tissue and Cell 38 (2006) 121-125
3. Borzacchiello G., and Roperto F. Double-labeling immunofluorescence: an alternative to detect multiple antigens in tissue sections. Veterinary Pathology 43 (2006) 83
4. Canese M.G., and Bussolati G. Immuno-electron-cytochemical localization of the somatostatin cells in the human antral mucosa. Journal of Histochemistry and Cytochemistry 25 (1977) 1111-1118
5. Jeurissen S.H., Claassen E., Boonstra-Blom A.G., Vervelde L., and Marga Janse E. Immunocytochemical techniques to investigate the pathogenesis of infectious micro-organisms and the concurrent immune response of the host. Developmental and Comparative Immunology 24 (2000) 142-151
6. Lechago J., Sun N.C., and Weinstein W.M. Simultaneous visualization of two antigens in the same tissue section by combining immunoperoxidase with immunofluorescence techniques. Journal of Histochemistry and Cytochemistry 27 (1979) 1221-1225
7. Mason D.Y., Abdulaziz Z., Falini B., and Stein H. Single and double immunoenzymatic techniques for labeling tissue sections with monoclonal antibodies. Annals of the New York Academy of Sciences 420 (1983) 127-133
8. Mason D.Y., Micklem K., and Jones M. Double immunofluorescence labelling of routinely processed paraffin sections. Journal of Pathology 191 (2000) 452-461
9. Masuda A., Nishikawa T., Yamamoto T., and Kobayashi M. Simple method for Photoshop-aided double immunohistochemistry - usage of 'image stack' function. Histopathology 53 (2008) 609-610
10. Masuda A., Nishikawa T., Yamamoto T., and Kobayashi M. Simple method for Photoshop-aided double immunohistochemistry - usage of 'image stack' function. Histopathology 53 (2008) 609-610
11. Nga, M.E., Lim, K.H., Tan E.Y., Chan, P., Tan, S.Y., Walford, N. 2008. Malignant adenomyoepithelial tumor of the breast: multi-immunolabeling technique and detailed immunophenotypic study. 16, 100-104.
12. Paciello O., Shelton G.D., and Papparella. Expression of major histocompatibility complex class I and class II antigens in canine masticatory muscle myositis. Neuromuscolar Disorders 17 (2007) 313-320
13. Ramalho L.N., Ribeiro-Silva A., Cassali G.D., and Zucoloto S. The expression of p63 and cytokeratin 5 in mixed tumors of the canine mammary gland provides new insights into the histogenesis of these neoplasms. Veterinary Pathology 43 (2006) 424-429
14. Ramos-Vara J.A. Technical aspects of immunohistochemistry. Veterinary Pathology 42 (2005) 405-426
15. Ramshaw A.L., and Parums D.V. Combined immunohistochemical and immunofluorescence method to determine the phenotype of proliferating cell populations. Journal of Clinical Pathology 45 (1992) 1015-1017
16. Tao Q., Srivastava G., Loke S.L., Chan E.Y., and Ho F.C. Improved double immunohistochemical staining method for cryostat and paraffin wax sections, combining alkaline phosphatase anti-alkaline phosphatase and indirect immunofluorescence. Journal of Clinical Pathology 47 (1994) 597-600
17. Valnes K., and Brandtzaeg P. Paired indirect immunoenzyme staining with primary antibodies from the same species. Application of horseradish peroxidase and alkaline phosphatase as sequential labels. The Histochemical Journal 16 (1984) 477-487
18. Van Vlierberghe R.L., Sandel M.H., Prins F.A., Van Iersel L.B., Van d.V.C.J., Tollenaar R.A., and Kuppen P.J. Four-color staining combining fluorescence and brightfield microscopy for simultaneous immune cell phenotyping and localization in tumor tissue sections. Microscopy Research and Technique 67 (2005) 15-21
19. Vandesande F. Double and multiple immunoenzymatic labelling of tissue sections for light microscopy. Acta Histochemica Supplementband 35 (1988) 107-115