Direct multiplex reverse transcription-nested PCR detection of influenza viruses without RNA purification

Journal of Microbiology and Biotechnology

Volumn 19, Issue 11, 2009, Pages 1470-1474

  Song, Man Ki ^a   Chang, Jun ^b   Hong, Yeongjin ^c   Hong, Sunghoi ^d   Kim, Suhng Wook ^d  

^a International Vaccine Institute   (South Korea)

^b Ewha Womans University   (South Korea)

^c Chonnam National University Medical School   (South Korea)

^d Korea University   (South Korea)

Author keywords

Influenza virus; Multiplex RT PCR

Indexed keywords

VIRUS RNA;

ARTICLE; CONTROLLED STUDY; INFLUENZA VIRUS; INFLUENZA VIRUS A H1N1; INFLUENZA VIRUS A H3N2; INFLUENZA VIRUS B; LABORATORY TEST; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA PURIFICATION; RNA VIRUS; SENSITIVITY AND SPECIFICITY; VIRUS DETECTION; VIRUS PARTICLE;

DNA, VIRAL; HOT TEMPERATURE; HUMANS; INFLUENZA A VIRUS, H1N1 SUBTYPE; INFLUENZA A VIRUS, H3N2 SUBTYPE; INFLUENZA B VIRUS; INFLUENZA, HUMAN; MOLECULAR DIAGNOSTIC TECHNIQUES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SALIVA; SENSITIVITY AND SPECIFICITY;

HUMAN INFLUENZA VIRUS; INFLUENZA B VIRUS; INFLUENZAVIRUS A; ORTHOMYXOVIRIDAE; RNA VIRUSES;

EID: 71549140668     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.0905.05012     Document Type: Article

References (15)

1. Chang, H. K., J. H. Park, M. S. Song, T. K. Oh, S. Y. Kim, C. J. Kim, et al. 2008. Development of multiplex RT-PCR assays for rapid detection and subtyping of influenza type A viruses from clinical specimens. J. Microbiol. Biotechnol. 18: 1164-1169.
2. Chi, X. S., F. Li, J. S. Tam, R. Rappaport, and S. M. Cheng. 2007. Semiquantitative one-step RT-PCR for simultaneous identification of human influenza and respiratory syncytial viruses. J. Virol. Methods 139: 90-92.
3. Choi, K. and S. B. Thacker. 1981. An evaluation of influenza mortality surveillance, 1962-1979. I. Time series forecasts of expected pneumonia and influenza deaths. Am. J. Epidemiol. 113: 215-226.
4. Choi, K. and S. B. Thacker. 1981. An evaluation of influenza mortality surveillance, 1962-1979. II. Percentage of pneumonia and influenza deaths as an indicator of influenza activity. Am. J. Epidemiol. 113: 227-235.
5. Collins, R. A., L. S. Ko, K. L. So, T. Ellis, L. T. Lau, and A. C. Yu. 2003. A NASBA method to detect high- and low-pathogenicity H5 avian influenza viruses. Avian Dis. 47: 1069-1074.
6. Daum, L. T., L. C. Canas, C. A. Schadler, V. A. Ujimori, W. B. Huff, W. J. Barnes, and K. L. Lohman. 2002. A rapid, single-step multiplex reverse transcription-PCR assay for the detection of human H1N1, H3N2, and B influenza viruses. J. Clin. Virol. 25: 345-350.
7. Doller, G., W. Schuy, K. Y. Tjhen, B. Stekeler, and H. J. Gerth. 1992. Direct detection of influenza virus antigen in nasopharyngeal specimens by direct enzyme immunoassay in comparison with quantitating virus shedding. J. Clin. Microbiol. 30: 866-869.
8. Ellis, J. S., D. M. Fleming, and M. C. Zambon. 1997. Multiplex reverse transcription-PCR for surveillance of influenza A and B viruses in England and Wales in 1995 and 1996. J. Clin. Microbiol. 35: 2076-2082.
9. Gharabaghi, F., R. Tellier, R. Cheung, C. Collins, G. Broukhanski, S. J. Drews, and S. E. Richardson. 2008. Comparison of a commercial qualitative real-time RT-PCR kit with direct immunofluorescence assay (DFA) and cell culture for detection of influenza A and B in children. J. Clin. Virol. 42: 190-193.
10. Imai, M., A. Ninomiya, H. Minekawa, T. Notomi, T. Ishizaki, M. Tashiro, and T. Odagiri. 2006. Development of H5-RT-LAMP (loop-mediated isothermal amplification) system for rapid diagnosis of H5 avian influenza virus infection. Vaccine 24: 6679-6682.
11. Ito, M., M. Watanabe, N. Nakagawa, T. Ihara, and Y. Okuno. 2006. Rapid detection and typing of influenza A and B by loop-mediated isothermal amplification: Comparison with immunochromatography and virus isolation. J. Virol. Methods 135: 272-275. (Pubitemid 43902977)
12. Payungporn, S., S. Chutinimitkul, A. Chaisingh, S. Damrongwantanapokin, C. Buranathai, A. Amonsin, A. Theamboonlers, and Y. Poovorawan. 2006. Single-step multiplex real-time RT-PCR for H5N1 influenza A virus detection. J. Virol. Methods 131: 143-147. (Pubitemid 43087644)
13. van Elden, L. J., M. Nijhuis, P. Schipper, R. Schuurman, and A. M. van Loon. 2001. Simultaneous detection of influenza viruses A and B using real-time quantitative PCR. J. Clin. Microbiol. 39: 196-200.
14. Yang, Y. G., J. Y. Kim, Y. H. Song, and D. S. Kim. 2007. A novel buffer system, AnyDirect, can improve polymerase chain reaction from whole blood without DNA isolation. Clin. Chim. Acta 380: 112-117.
15. Yang, Y. G., M. K. Song, S. J. Park, and S. W. Kim. 2007. Direct detection of Shigella flexneri and Salmonella Typhimurium in human feces by real-time PCR. J. Microbiol. Biotechnol. 17: 1616-1621.