Complementary retrieval of 16S rRNA gene sequences using broad-range primers with inosine at the 3′-terminus: Implications for the study of microbial diversity

FEMS Microbiology Ecology

Volumn 71, Issue 1, 2010, Pages 157-167

  Brands, Berit ^a   Vianna, Morgana E ^a,b   Seyfarth, Ilse ^a   Conrads, Georg ^a   Horz, Hans Peter ^a  

^a RWTH AACHEN UNIVERSITY   (Germany)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Biased amplification; Broad range PCR; Inosine primers; Microbial diversity; T RFLP analysis

Indexed keywords

BACTERIAL RNA; INOSINE; RNA 16S;

BIOFILM; DETECTION METHOD; GENE EXPRESSION; MICROBIAL COMMUNITY; POLYMERASE CHAIN REACTION; RNA; SPECIES DIVERSITY; SPECIES RICHNESS;

3' UNTRANSLATED REGION; ACTINOMYCES; ACTINOMYCES NAESLUNDII; AGGREGATIBACTER APHROPHILUS; ARTICLE; BACTERIUM; BACTEROIDES; BINDING SITE; BIOFILM; CAMPYLOBACTER RECTUS; CATONELLA MORBI; CONTROLLED STUDY; DEFERRIBACTER; EUBACTERIUM; FILIFACTOR ALOCIS; FLEXISTIPES; FUSOBACTERIUM NUCLEATUM; GENE AMPLIFICATION; GENE SEQUENCE; LAUTROPIA MIRABILIS; LEPTOTRICHIA; LEPTOTRICHIA BUCCALIS; METHANOBREVIBACTER; MICROBIAL COMMUNITY; MICROBIAL DIVERSITY; MOLECULAR CLONING; MOUTH PLAQUE; NEISSERIA; NONHUMAN; NUCLEOTIDE SEQUENCE; OLSENELLA PROFUSA; PEPTOSTREPTOCOCCUS; PORPHYROMONAS GINGIVALIS; PREVOTELLA INTERMEDIA; PRIORITY JOURNAL; REPRODUCIBILITY; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; STREPTOCOCCUS CONSTELLATUS; TANNERELLA FORSYTHENSIS; THERMOSTABILITY; TREPONEMA; VEILLONELLA PARVULA;

CASE-CONTROL STUDIES; CLONING, MOLECULAR; CLUSTER ANALYSIS; DENTAL PLAQUE; DNA PRIMERS; GENES, BACTERIAL; GENETIC VARIATION; HUMANS; INOSINE; PERIODONTAL POCKET; PERIODONTITIS; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S;

EID: 71549139483     PISSN: 01686496     EISSN: 15746941     Source Type: Journal    
DOI: 10.1111/j.1574-6941.2009.00786.x     Document Type: Article

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