Purification and biochemical characterization of a mycelial glucose- and xylose-stimulated β-glucosidase from the thermophilic fungus Humicola insolens

Process Biochemistry

Volumn 45, Issue 2, 2010, Pages 272-278

  Souza, Flávio Henrique Moreira ^a   Nascimento, Cesar Vanderlei ^a   Rosa, José César ^a   Masui, Douglas Chodi ^a   Leone, Francisco Assis ^a   Jorge, João Atílio ^a   Furriel, Rosa Prazeres Melo ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Glucosidase; Glycosidase; Cellobiase; Glucose stimulated glucosidase; Glucose stimulated glycosidase; Humicola insolens

Indexed keywords

AMINO ACID SEQUENCE; BIOCHEMICAL CHARACTERIZATION; CARBOHYDRATE CONTENT; CATALYTIC EFFICIENCIES; CELLOBIOSE; CELLULOSIC MATERIAL; ELEVATED CONCENTRATIONS; ENZYME SUBSTRATES; GALACTOPYRANOSIDE; GEL FILTRATION; GLUCOPYRANOSIDE; GLUCOSIDASE; GLYCOSIDASES; HOMODIMERS; KINETIC STUDY; MASS SPECTROMETRY ANALYSIS; NATIVE ENZYMES; NATURAL SUBSTRATES; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; STIMULATORY EFFECTS; THERMAL STABILITY; THERMOPHILIC FUNGUS; THERMOPHILIC MOLD;

AMINES; AMINO ACIDS; CARBOHYDRATES; ELECTROPHORESIS; ENZYME ACTIVITY; ENZYMES; GELS; HYDROLYSIS; MASS SPECTROMETRY; ORGANIC ACIDS; PURIFICATION; SODIUM; SODIUM SULFATE; SUBSTRATES;

GLUCOSE;

FUNGI; HUMICOLA GRISEA VAR. THERMOIDEA; HUMICOLA INSOLENS;

EID: 71249142277     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2009.09.018     Document Type: Article

References (43)

1. Kim J., and Yun S. Discovery of cellulose as a smart material. Macromolecules 39 (2006) 4202-4206
2. Beguin P., and Aubert J.P. The biological degradation of cellulose. FEMS Microbiol Rev 13 (1994) 25-58
3. Kumar R., Singh S., and Singh O.V. Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives. J Ind Microbiol Biotechnol 35 (2008) 377-391
4. Bhatia Y., Mishra S., and Bisaria V.S. Microbial β-glucosidases: cloning, properties, and applications. Crit Rev Biotechnol 22 (2002) 375-407
5. Lynd L.R., Weimer P.J., Zyl W.H., and Pretorius I.S. Microbial cellulose utilization: fundamentals and biotechnology. Microbiol Mol Biol Rev 66 (2002) 506-577
6. Bhat M., and Bhat T.S. Cellulose degrading enzymes and their potential industrial applications. Biotechnol Adv 15 (1997) 583-620
7. Yang S., Jiang Z., Yan Q., and Zhu H. Characterization of a thermostable extracellular β-glucosidase with activities of exoglucanase and transglycosylation from Paecilomyces thermophila. J Agric Food Chem 56 (2008) 602-608
8. Nakkharat P., and Haltrich D. Purification and characterization of an intracellular enzyme with β-glucosidase and β-galactosidase activity from the thermophilic fungus Talaromyces thermophilus CBS 236.58. J Biotechnol 123 (2006) 304-313
9. Kaur J., Chadha B.S., Kumar B.A., Ghatora S.K., and Saini H.S. Purification and characterization of β-glucosidase from Melanocarpus sp. MTCC 3922. Electron J Biotechnol 10 (2007) 260-270
10. Karnchanatat A., Petsom A., Sangvanich P., Piaphukiew J., Whalley A.J., Reynolds C.D., et al. Purification and biochemical characterization of an extracellular beta-glucosidase from the wood-decaying fungus Daldinia eschscholzii (Ehrenb.: Fr.) Rehm. FEMS Microbiol Lett 270 (2007) 162-170
11. Bhiri F., Chaabouni S.E., Limam F., Ghrir R., and Marzouki N. Purification and biochemical characterization of extracellular β-glucosidases from the hypercellulolytic Pol6 mutant of Penicillium occitanis. Appl Biochem Biotechnol 149 (2008) 169-182
12. Riou C., Salmon J.M., Vallier M.J., Günata Z., and Bare P. Purification, characterization and substrate specificity of a novel highly glucose-tolerant β-glucosidase from Aspergillus oryzae. Appl Environ Microbiol 64 (1998) 3607-3614
13. Zanoelo F.F., Polizeli M.L.T.M., Terenzi H.F., and Jorge J.A. β-Glucosidase activity from the thermophilic fungus Scytalidium thermophilum is stimulated by glucose and xylose. FEMS Microbiol Lett 240 (2004) 137-143
14. Sonia K.G., Chadha B.S., Badhan A.K., Saini H.S., and Bhat M.K. Identification of glucose tolerant acid active β-glucosidases from thermophilic and thermotolerant fungi. World J Microbiol Biotechnol 24 (2008) 599-604
15. Peralta R.M., Kadowaki M.K., Terenzi H.F., and Jorge J.A. A highly thermostable β-glucosidase activity from the thermophilic fungus H. grisea var. thermoidea: purification and biochemical characterization. FEMS Microbiol Lett 146 (1997) 291-295
16. Venturi L.L., Polizeli M.L., Terenzi H.F., Furriel R.P.M., Jorge J.A., and Extracellular. β-d-glucosidase from Chaetomium thermophilum var. coprophilum: production, purification and some biochemical properties. J Basic Microbiol 42 (2002) 55-66
17. Mashewari R., Bharadwaj G., and Bhat M.K. Thermophilic fungi: their physiology and enzymes. Microbiol Mol Biol Rev 64 (2000) 461-488
18. Cooney D.G., and Emerson R. Thermophilic fungi (1964), WH Freeman, San Francisco pp. 73-79
19. Vogel H.J. Distribution of lysine pathways among fungi: evolutionary implications. Am Nat 98 (1964) 435-446
20. Bergmeyer H.U., and Bernt E. In: Bergmeyer H.U. (Ed). Methods of enzymatic analysis vol. 3 (1974), Verlag Chimie-Academic Press, New York 1205-1215
21. Miller G.L. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal Chem 31 (1959) 426-428
22. Davis B.J. Disc electrophoresis. II. Method and application to human serum proteins. Ann N Y Acad Sci 121 (1964) 404-427
23. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
24. Peralta R.M., Terenzi H.F., and Jorge J.A. β-Glycosidase activities of Humicola grisea: biochemical and kinetic characterization of a multifunctional enzyme. Biochim Biophys Acta 1033 (1990) 243-249
25. Segel I.H. Enzyme kinetics. Behavior and analysis of rapid equilibrium and steady-state enzyme systems (1975), John Willey & Sons
26. McIlvaine T.C. A buffer solution for colorimetric comparison. J Biol Chem 49 (1921) 183-186
27. Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., and Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem 28 (1956) 350-356
28. Read S.M., and Northcote D.H. Minimization of variation in the response to different protein of the Coomassie blue G dye-binding assay for protein. Anal Biochem 116 (1981) 53-64
29. Leone F.A., Baranauskas J.A., Furriel R.P.M., and Borin I.A. SigrafW: an easy-to-use program for fitting enzyme kinetic data. Biochem Mol Biol Educ 33 (2005) 399-403
30. Rao U.S., and Murthy S.K. Purification and characterization of a beta-glucosidase and endocellulase from Humicola insolens. Indian Biochem Biophys 25 (1988) 687-694
31. Lin J., Pillay B., and Singh S. Purification and biochemical characteristics of β-d-glucosidase from a thermophilic fungus Thermomyces lanuginosus-SSBP. Biotechnol Appl Biochem 30 (1999) 81-87
32. Hayashida S., Ohta K., and Mo K. Cellulases of Humicola insolens and Humicola grisea. Methods Enzymol 160 (1988) 323-332
33. Saibi W., Amouri B., and Gargouri A. Purification and biochemical characterization of a transglucosilating β-glucosidase of Stachybotrys strain. Appl Microbiol Biotechnol 77 (2007) 293-300
34. Parry N.J., Beever D.E., Owen E., Vandenberghe I., Van Beeumen J., and Bhat M.K. Biochemical characterization and mechanisms of action of a thermostable β-glucosidase purified from Thermoascus aurantiacus. Biochem J 353 (2001) 117-127
35. Osaki H., and Yamada K. Isolation of Streptomyces sp. producing glucose-tolerant β-glucosidases and properties of the enzymes. Agric Biol Chem 55 (1991) 979-987
36. Harchand R.K., and Singh S. Characterization of cellulose complex of Streptomyces albaduncus. J Basic Microbiol 37 (1997) 93-103
37. Saha B.C., and Bothast R.J. Production, purification and characterization of a highly glucose-tolerant novel β-glucosidase from Candida peltata. Appl Environ Microbiol 61 (1996) 3165-3170
38. Saha B.C., and Bothast R.J. Glucose tolerant and thermophilic β-glucosidases from yeasts. Biotechnol Lett 18 (1996) 155-158
39. Wright R.M., Yablonsky M.D., Shalita Z.P., Goyal A.K., and Eveleigh D.E. Cloning, characterization, nucleotide sequence of a gene encoding Microbispora BglB, a thermostable β-glucosidase expressed in Escherichia coli. Appl Environ Microbiol 58 (1992) 3455-3465
40. Perez-Pons J.A., Rebordosa X., and Querol E. Properties of a novel glucose-enhanced β-glucosidase purified from Streptomyces sp. (ATCC 11238). Biochim Biophys Acta 1251 (1995) 145-153
41. Decker C.H., Visser J., and Schreier P. β-Glucosidase multiplicity from Aspergillus tubingiensis CBS 643.92: purification and characterization of four β-glucosidases and their differentiation with respect to substrate specificity, glucose inhibition and acid tolerance. Appl Microbiol Biotechnol 55 (2001) 157-163
42. Straatsma G., and Samson R.A. Taxonomy of Scytalidium thermophilum, an important thermophilic fungus in mushroom compost. Mycol Res 97 (1993) 321-328
43. Marchessault R.H., and Sundararajan P.R. Cellulose. In: Aspinall G.O. (Ed). The polysaccharides vol. 2 (1993), Academic Press Inc., New York 11-95