One-step purification of YLLIP2 isoforms from Candida sp. 99-125 by polyethyleneimine modified poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) monolith

Journal of Molecular Catalysis B: Enzymatic

Volumn 62, Issue 2, 2010, Pages 149-154

  Lv, Yong Qin ^a   Fu, Da Yan ^a   Tan, Tian Wei ^a   Wang, Man Yi ^a  

^a BEIJING UNIVERSITY OF CHEMICAL TECHNOLOGY   (China)

Author keywords

Ion exchange; Isoform; Lipase; Monolith; Optimization; Polyethyleneimine

Indexed keywords

BACK PRESSURES; CANDIDA SP. 99-125; CONCENTRATION OF; CRUDE EXTRACT; CRUDE LIPASE; DENATURING PAGE; ETHYLENE GLYCOL DIMETHACRYLATE; EXTRACELLULAR LIPASE; FAST SEPARATION; FUNCTIONALIZATIONS; FUNCTIONALIZED; GLYCIDYL METHACRYLATE; IN-SITU; ION-EXCHANGE CHROMATOGRAPHY; ISOFORM; ISOFORMS; MALDI TOF MS; MONOLITHIC COLUMNS; ONE-STEP PURIFICATION; POLYETHYLENEIMINE; POROGENS; SINGLE-STEP; VOLUME RATIO; YARROWIA LIPOLYTICA;

ACRYLIC MONOMERS; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; ETHYLENE; ETHYLENE GLYCOL; ION EXCHANGE; ION EXCHANGERS; IONS; MOLECULAR MASS; OPTIMIZATION; PURIFICATION; SEPARATION;

MONOLITHIC INTEGRATED CIRCUITS;

COPOLYMER; ETHYLENE GLYCOL DIMETHACRYLATE; GLYCIDYL METHACRYLATE; HEPTANE; ISOENZYME; METHACRYLIC ACID DERIVATIVE; POLY(GLYCIDYL METHACRYLATE CO ETHYLENE GLYCOL DIMETHACRYLATE); POLYETHYLENEIMINE; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

ARTICLE; CANDIDA; CHEMICAL MODIFICATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME IMMOBILIZATION; ENZYME ISOLATION; ENZYME PURIFICATION; ION EXCHANGE CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERIZATION; TEMPERATURE; YARROWIA LIPOLYTICA;

CANDIDA; YARROWIA LIPOLYTICA;

EID: 71249140173     PISSN: 13811177     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molcatb.2009.10.001     Document Type: Article

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