Quick recovery and characterization of cell-free DNA in seminal plasma of normozoospermia and azoospermia: Implications for non-invasive genetic utilities

Asian Journal of Andrology

Volumn 11, Issue 6, 2009, Pages 703-709

  Li, Hong Gang ^a   Huang, Shi Yun ^a   Zhou, Hui ^a   Liao, Ai Hua ^a   Xiong, Cheng Liang ^a  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CELL FREE DNA; DNA; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; AZOOSPERMIA; CHROMOSOME DELETION Y; CONTROLLED STUDY; DNA FRAGMENTATION; DNA ISOLATION; DNA PURIFICATION; ELECTROPHORESIS; GENE AMPLIFICATION; HUMAN; HUMAN CELL; MALE; NUCLEOTIDE SEQUENCE; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SEMINAL PLASMA;

AZOOSPERMIA; CHROMOSOMES, HUMAN, Y; DNA; GENE DELETION; HUMANS; MALE; SEMEN;

EID: 71049195760     PISSN: 1008682X     EISSN: 17457262     Source Type: Journal    
DOI: 10.1038/aja.2009.65     Document Type: Article

References (24)

1. Gonzales GF. Function of seminal vesicles and their role on male fertility. Asian J Androl 2001; 3: 251-8.
2. Schill WB, Henkel R. Advancement in biochemical assays in andrology. Asian J Androl 1999; 1: 45-51.
3. Vlassov VV, Laktionov PP, Rykova EY. Extracellular nucleic acids. Bioessays 2007; 29: 654-67.
4. Stroun M, Lyautey J, Lederrey C, Olson-Sand A, Anker P. About the possible origin and mechanism of circulating DNA apoptosis and active DNA release. Clin Chim Acta 2001; 313: 139-42.
5. Fleischhacker M, Schmidt B. Circulating nucleic acids (CNAs). Biochim Biophys Acta 2007; 1775: 181-232.
6. Swarup V, Rajeswari MR. Circulating (cell-free) nucleic acids - a promising, non-invasive tool for early detection of several human diseases. FEBS Lett 2007; 581: 795-9.
7. Chan KC, Lai PB, Mok TS, Chan HL, Ding C, et al. Quantitative analysis of circulating methylated DNA as a biomarker for hepatocellular carcinoma. Clin Chem 2008; 54: 1528-36.
8. Altimari A, Grigioni AD, Benedettini E, Gabusi E, Schiavina R, et al. Diagnostic role of circulating free plasma DNA detection in patients with localized prostate cancer. Am J Clin Pathol 2008; 129: 756-62.
9. Lapaire O, Bianchi DW, Peter I, O'Brein B, Stroh H, et al. Cell-free fetal DNA in amniotic fluid: Unique fragmentation signatures in euploid and aneuploid fetuses. Clin Chem 2007; 53: 405-11.
10. Zubakov D, Hanekamp E, Kokshoorn M, van IJcken W, Kayser M. Stable RNA markers for identification of blood and saliva stains revealed from whole genome expression analysis of time-wise degraded samples. Int J Legal Med 2007; 122: 135-42.
11. Schmidt B, Carstensen T, Engel E, Jandrig B, Witt C, et al. Detection of cell-free nucleic acids in bronchial lavage fluid supernatants from patients with lung cancer. Euro J Cancer 2004; 40: 452-60.
12. Wong LJ, Lueth M, Li XN, Lau CC, Vogel H. Detection of mitochondrial DNA mutations in the tumor and cerebrospinal fluid of medulloblastoma patients. Cancer Res 2003; 63: 3866-71.
13. Hoque MO, Begum S, Topaloglu O, Chatterjee A, Rosenbaum E, et al. Quantitation of promoter methylation of multiple genes in urine DNA and bladder cancer detection. J Natl Cancer Inst 2006; 98: 996-1004.
14. Chou JS, Jacobson JD, Patton WC, King A, Chan PJ. Modified isocratic capillary electrophoresis detection of cell-free DNA in semen. J Assist Reprod Genet 2004; 21: 397-400.
15. WHO (World Health Organization). WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interactions, 4th edn. Cambridge: Cambridge University Press; 1999.
16. Li HG, Ding XF, Liao AH, Kong XB, Xiong CL. Expression of CatSper family transcripts in the mouse testis during post-natal development and human ejaculated spermatozoa: Relationship to sperm motility. Mol Hum Reprod 2007; 3: 299-306.
17. Li HG, Ding XF, Wang C, Zhao JX, Zuo MD, et al. Detection of Y chromosome microdeletions in semen of 241 patients with azoospermia. Chin J Med 2008; 88: 1662-5.
18. García Moreira V, Prieto García B, de la Cera Martínez T, Alvarez Menéndez FV. Elevated transrenal DNA (cell-free urine DNA) in patients with urinary tract infection compared to healthy controls. Clin Biochem 2009; 42: 729-31.
19. Su YH, Wang M, Brenner DE, Ng A, Melkonyan H, et al. Human urine contains small, 150-250 nucleotide-sized, soluble DNA derived from the circulation and may be useful in the detection of colorectal cancer. J Mol Diagn 2004; 6: 101-7.
20. Brinkmann V, Reichard U, Goosmann C, Fauler B, Uhlemann Y, et al. Neutrophil extracellular traps kill bacteria. Science 2004; 303: 1532-5.
21. Blanco-Rodríguez J. A matter of death and life: The significance of germ cell death during spermatogenesis. Int J Androl 1998; 21: 236-48.
22. Quinn PJ. Deoxyribonuclease activity in semen. J Reprod Fertil 1968; 17: 35-9.
23. 2+-dependent endonuclease. J Biol Chem 1982; 257: 13114-9.
24. Jahr S, Hentze H, Englisch S, Hardt D, Fackelmayer FO, et al. DNA fragments in the blood plasma of cancer patients: Quantitations and evidence for their origin from apoptotic and necrotic cells. Cancer Res 2001; 61: 1659-65.