TROSY-selected ZZ-exchange experiment for characterizing slow chemical exchange in large proteins

Journal of Biomolecular NMR

Volumn 45, Issue 4, 2009, Pages 357-360

  Li, Ying ^a   Palmer III, Arthur G ^a  

^a Columbia University ^*  (United States)

Author keywords

Cadherin; Chemical exchange; Kinetic rates; TROSY; ZZ exchange

Indexed keywords

CADHERIN;

ARTICLE; CHEMICAL ANALYSIS; CHEMICAL EXCHANGE; CROSS LINKING; DIMERIZATION; EXTRACELLULAR SPACE; IONIC STRENGTH; MAGNETISM; MOLECULAR WEIGHT; PRIORITY JOURNAL;

CADHERINS; HUMANS; KINETICS; MAGNETIC RESONANCE SPECTROSCOPY; NITROGEN ISOTOPES; PROTEIN STRUCTURE, TERTIARY; PROTEINS;

EID: 71049172775     PISSN: 09252738     EISSN: 15735001     Source Type: Journal    
DOI: 10.1007/s10858-009-9385-0     Document Type: Article

References (18)

1. 15N longitudinal decay and chemical exchange rates of systems in slow equilibrium J Biomol NMR 4 727 734 10.1007/BF00404280
2. 1H-NMR thermometer suitable for cryoprobes Magn Reson Chem 45 175 178 10.1002/mrc.1941
3. PM Hwang LE Kay 2005 Solution structure and dynamics of integral membrane proteins by NMR: a case study involving the enzyme PagP Methods Enzymol 394 335 350 10.1016/S0076-6879(05)94013-5 (Pubitemid 40460922)
4. 1ρ experiment with improved sensitivity for medium- and high-molecular-weight proteins J Am Chem Soc 128 8110 8111 10.1021/ja061692f (Pubitemid 43967735)
5. 1) for quantitation of fast exchange processes in large proteins J Am Chem Soc 125 12064 12065 10.1021/ja037101s (Pubitemid 37238821)
6. DM Korzhnev K Kloiber V Kanelis V Tugarinov LE Kay 2004 Probing slow dynamics in high molecular weight proteins by methyl-TROSY NMR spectroscopy: application to a 723-residue enzyme J Am Chem Soc 126 3964 3973 10.1021/ja039587i (Pubitemid 38391889)
7. CD Kroenke JP Loria LK Lee M Rance AG Palmer 1998 Longitudinal and transverse 1H-15 N dipolar 15 N/chemical shift anisotropy relaxation interference: unambiguous determination of rotational diffusion tensors and chemical exchange effects in biological macromolecules J Am Chem Soc 120 7905 7915 10.1021/ja980832l (Pubitemid 28392074)
8. JP Loria M Rance AG Palmer 1999 A TROSY CPMG sequence for characterizing chemical exchange in large proteins J Biomol NMR 15 151 155 10.1023/A: 1008355631073
9. A Meissner JO Duus OW Sørensen 1997 Spin-state-selective excitation. Application for E.COSY-type measurement of JHH coupling constants J Magn Reson 128 92 97 10.1006/jmre.1997.1213 1997JMagR.128...92M
10. VZ Miloushev F Bahna C Ciatto G Ahlsen B Honig L Shapiro AG Palmer 2008 Dynamic properties of a type II cadherin adhesive domain: implications for the mechanism of strand-swapping of classical cadherins Structure 16 1195 1205 10.1016/j.str.2008.05.009
11. GT Montelione G Wagner 1989 2D chemical exchange NMR spectroscopy by proton-detected heteronuclear correlation J Am Chem Soc 111 3096 3098 10.1021/ja00190a072
12. Mosteller F, Tukey JW (1977) Data analysis and regression. A second course in statistics. Addison-Wesley, Reading, MA
13. D Sahu GM Clore J Iwahara 2007 TROSY-based z-exchange spectroscopy: application to the determination of the activation energy for intermolecular protein translocation between specific sites on different DNA molecules J Am Chem Soc 129 13232 13237 10.1021/ja074604f (Pubitemid 350058364)
14. V Sklenar M Piotto R Leppik V Saudek 1993 Gradient-tailored water suppression for 1H-15 N HSQC experiments optimized to retain full sensitivity J Magn Reson A 102 241 245 10.1006/jmra.1993.1098
15. MD Sørensen A Meissner OW Sørensen 1997 Spin-state-selective coherence transfer via intermediate states of two-spin coherence in IS spin systems: application to E.COSY-type measurement of J coupling constants J Biomol NMR 10 181 186 10.1023/A:1018323913680
16. C Wang M Rance AG Palmer 2003 Mapping chemical exchange in proteins with MW > 50 kD J Am Chem Soc 125 8968 8969 10.1021/ja035139z (Pubitemid 36903790)
17. G Wider D Neri K Wüthrich 1991 Studies of slow conformational equilibria in macromolecules by exchange of heteronuclear longitudinal 2-spin-order in a 2D difference correlation experiment J Biomol NMR 1 93 98 10.1007/BF01874572
18. G Zhu Y Xia LK Nicholson KH Sze 2000 Protein dynamics measurements by TROSY-based NMR experiments J Magn Reson 143 423 426 10.1006/jmre.2000.2022 2000JMagR.143..423Z