Preparation of soluble GST fusion proteins

Cold Spring Harbor Protocols

Volumn 4, Issue 11, 2009, Pages

  Rebay, Ilaria ^a   Fehon, Richard G ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

AGAROSE; GLUTATHIONE; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN;

ARTICLE; BACTERIAL STRAIN; CENTRIFUGATION; GENE VECTOR; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN ELECTROPHORESIS; PROTEIN ISOLATION; PROTEIN SYNTHESIS; SOLUBILITY;

BACTERIA (MICROORGANISMS);

EID: 70949102842     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot4996     Document Type: Article

References (17)

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2. Einarson MB, Pugacheva EN, Orlinick JR. 2007b. Identification of protein-protein interactions with glutathione-S-transferase (GST) fusion proteins. Cold Spring Harb Protoc doi: 10.1101/pdb.top11.
3. Einarson MB, Pugacheva EN, Orlinick JR2007c. GST pull-down. Cold Spring Harb Protoc doi: 10.1101/pdb.prot4757.
4. Einarson MB, Pugacheva EN, Orlinick JR. 2007d. Far Western: Labeling GST fusion proteins. Cold Spring Harb Protoc doi: 10.1101/ pdb.prot4758.
5. Einarson MB, Pugacheva EN, Orlinick JR. 2007e. Far Western: Probing membranes. Cold Spring Harb Protoc doi: 10.1101/pdb.prot4759.
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10. McCartney BM, Fehon RG. 1996. Distinct cellular and subcellular patterns of expression imply distinct functions for the Drosophila homologues of moesin and the neurofibromatosis 2 tumor suppressor, merlin. J Cell Biol 133: 843-852.
11. O'Neill EM, Rebay I, Tjian R, Rubin GM. 1994. The activities of two Ets-related transcription factors required for Drosophila eye development are modulated by the Ras/MAPK pathway. Cell 78: 137-147.
12. Rebay I, Fehon RG. 2009. Preparation of insoluble GST fusion proteins. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot4997.
13. Rebay I, Rubin GM. 1995. Yan functions as a general inhibitor of differentiation and is negatively regulated by activation of the Ras1/ MAPK pathway. Cell 81: 857-866.
14. Sambrook J, Russell DW. 2006a. Detection of protein-protein interactions using the GST fusion protein pulldown technique. Cold Spring Harb Protoc doi: 10.1101/pdb.prot3757.
15. Sambrook J, Russell DW. 2006b. Detection of protein-protein interactions using far western with GST fusion proteins. Cold Spring Harb Protoc doi: 10.1101/pdb.prot4014.
16. Sambrook J, Russell DW. 2006c. Purification of fusion proteins by affinity chromatography on glutathione agarose. Cold Spring Harb Protoc doi: 10.1101/pdb.prot3655.
17. Simpson RJ. 2006. SDS-PAGE of proteins. Cold Spring Harb Protoc doi: 10.1101/pdb.prot4313.