Construction of gene-targeting vectors by recombineering

Cold Spring Harbor Protocols

Volumn 4, Issue 9, 2009, Pages

  Lee, Song choon ^a   Liu, Pentao ^a  

^a WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA;

ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; DNA ISOLATION; ELECTROPORATION; ESCHERICHIA COLI; GENE CASSETTE; GENE TARGETING; GENE VECTOR; GENETIC ENGINEERING; GENETIC RECOMBINATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; KNOCKOUT MOUSE; METHODOLOGY; NONHUMAN; POLYMERASE CHAIN REACTION; RECOMBINANT PLASMID; REPORTER GENE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 70949101008     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5291     Document Type: Article

References (6)

1. Chan W, Costantino N, Li R, Lee SC, Su Q, Melvin D, Court DL, Liu P. 2007. A recombineering based approach for high-throughput conditional knockout targeting vector construction. Nucleic Acids Res 35: e64. doi: 10.1093/nar/gkm163.
2. Datsenko KA, Wanner BL. 2000. One-step inactivation of chromosomal genes in Escherichia coli K- 12 using PCR products. Proc Natl Acad Sci 97: 6640-6645.
3. Liu P, Jenkins NA, Copeland NG. 2003. A highly efficient recombineering-based method for generating conditional knockout mutations. Genome Res 13: 476-484.
4. Wang J, Sarov M, Rientjes J, Fu J, Hollak H, Kranz H, Xie W, Stewart AF, Zhang Y. 2006. An improved recombineering approach by adding RecA to λ Red recombination. Mol Biotechnol 32: 43-53.
5. Yu D, Ellis HM, Lee EC, Jenkins NA, Copeland NG, Court DL. 2000. An efficient recombination system for chromosome engineering in Escherichia coli. Proc Natl Acad Sci 97: 5978-5983.
6. Zhang Y, Buchholz F, Muyrers JP, Stewart AF. 1998. A new logic for DNA engineering using recombination in Escherichia coli. Nat Genet 20: 123-128.