Immunohistochemical and biochemical assay of versican in human sound predentine/dentine matrix

European Journal of Histochemistry

Volumn 53, Issue 3, 2009, Pages 125-133

  Ruggeri, A ^a   Orsini, G ^b   Mazzoni, A ^a   Nato, F ^a,c   Papa, V ^a   Piccirilli, M ^d   Putignano, A ^b   Mazzotti, G ^a   De Stefano Dorigo, E ^e   Breschi, Lorenzo ^e,f  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b UNIVERSITÀ POLITECNICA DELLE MARCHE   (Italy)

^c UNIVERSITY OF URBINO   (Italy)

^d UNIVERSITY OF CHIETI   (Italy)

^e UNIVERSITY OF TRIESTE   (Italy)

^f CNR   (Italy)

Author keywords

Dentine matrix; FEISEM; Fluorescence microscope; Immunohistochemistry; TEM; Versican

Indexed keywords

DENTIN MATRIX PROTEIN 1; FLUORESCENT DYE; GOLD; MONOCLONAL ANTIBODY; VERSICAN;

ADULT; ARTICLE; BIOASSAY; BIOCHEMISTRY; CONTROLLED STUDY; CORRELATION ANALYSIS; DEMINERALIZATION; DENTIN; ELECTRON MICROSCOPE; EMBEDDING; FIELD EMISSION SCANNING ELECTRON MICROSCOPY; FLUORESCENCE MICROSCOPE; HUMAN; HUMAN TISSUE; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; PROTEIN LOCALIZATION; TISSUE SECTION; TOOTH; TRANSMISSION ELECTRON MICROSCOPY; WESTERN BLOTTING;

ADULT; DENTAL PULP; DENTIN; HUMANS; IMMUNOHISTOCHEMISTRY; MICROSCOPY, ELECTRON, SCANNING; MICROSCOPY, ELECTRON, TRANSMISSION; MICROSCOPY, FLUORESCENCE; MOLAR; VERSICANS;

EID: 70849129283     PISSN: 1121760X     EISSN: None     Source Type: Journal    
DOI: 10.4081/ejh.2009.125     Document Type: Article

References (42)

1. Ababneh KT, Hall RC, Embery G.The proteoglycans of human cementum: immunohistochemical localization in healthy, periodontally involved and ageing teeth. J Periodontal Res 1999;34:87-96.
2. Apte SS. A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motifs: the ADAMTS family. Int J Biochem Cell Biol 2004;36:981-985
3. Bartold PM, Moule AJ, Li H, Rigby P. Isolation and characterization of the proteoglycans synthesized by adult human pulp fibroblasts in vitro. Int Endo Jour 1995;28:163-171
4. Breschi L, Lopes M, Gobbi P, Mazzotti G, Falconi M, Perdigão J. Dentin proteoglycans: an immunocytochemical FEISEM study. J Biomed Mater Res 2002;61:40-46 (Pubitemid 34650254)
5. Breschi L,Perdigão J, Lopes MM, Gobbi P, Mazzotti G. Morphological study of resin-dentin bonding with TEM and in-lens FESEM. Am J Dent 2003;16:267-274 (Pubitemid 41310068)
6. Cheng H, Caterson B,Yamauchi M. Identification and immunolocalization of chondroitin sulfate proteoglycans in tooth cementum. Connect Tissue Res 1999;40:37-47. (Pubitemid 29139346)
7. Embery G, Hall RC, Waddington RJ, Septier D, Goldberg M. Proteoglycans in dentinogenesis. Crit Rev Oral Biol Med 2001;12:331-349
8. Fisher LW, Termine JD, Dejter SW Jr, Whitson SW, Yanagishita M, Kimura JH, Hascall VC, Kleinman HK, Hassell JR, Nilsson B. Proteoglycans of developing bone. J Biol Chem 1983;258:6588-6594
9. Hirohata S. ADAMTS family-new extracellular matrix degrading enzyme. Seikagaku 2001;73:1333-1337 (Pubitemid 33723079)
10. Ilic MZ, Carter P,Tyndall A, Dudhia J, Handley CJ Proteoglycans and catabolic products of proteoglycans present in ligament. Biochem J 2005; 385(Pt 2):381-388 (Pubitemid 40165070)
11. Iozzo RV.The biology of the small leucine-rich proteoglycans: functional network of interactive proteins. J Biol Chem 1999; 274:18843-18846
12. Iozzo RV.The family of the small leucine-rich proteoglycans: key regulators of matrix assembly and cellular growth. Crit Rev Biochem Mol Biol 1997;32:141-174
13. Landolt RM,Vaughan L,Winterhalter KH, Zimmermann DR.Versican is selectively expressed in embryonic tissues that act as barriers to neural crest cell migration and axon outgrowth. Develo 1995;121:2303-2312
14. Marks SC Jr, Schroeder HE.Tooth eruption:Theories and facts. Anat Res 1996;245:374-393
15. Maruya Y, Sasano Y, Takahashi I, Kagayama M, Mayanagi H. Expression of extracellular matrix molecules,MMPs and TIMPs in alveolar bone, cementum and periodontal ligaments during rat tooth eruption. J Electron Microsc 2003;52:593-604. (Pubitemid 38092548)
16. Mazzoni A,Pashley DH, Nishitani Y, Breschi L, Mannello F,Tjäderhane L, et al. Reactivation of inactivated endogenous proteolytic activities in phosphoric acid-etched dentine by etch-and-rinse adhesives. Biomaterials 2006;27:4470-4476 (Pubitemid 44255659)
17. Mazzoni A, Mannello F, Tay FR, Tonti G, Papa S, Mazzotti G, et al. Zymographic analysis and characterization of MMP-2 and -9 isoforms in human sound dentin. Journal of Dental Research 2007; 86:436-440
18. Mazzoni A, Pashley DH,Tay FR, Gobbi P, Orsini G, Ruggeri A Jr, et al. Immunohistochemical identification of MMP-2 and MMP-9 in human dentin: Correlative FEI-SEM/TEM analysis. J Biomed Mater Res A 2009:88:697-703.
19. Neame PJ, Kay CJ, McQuillan DJ, Beales MP, Hassell JR. Independent modulation of collagen fibrillogenesis by decorin and lumican. Cell Mol Life Sci 2000;57:859-863
20. Orsini G, Ruggeri A Jr, Mazzoni A, Papa V, Mazzotti G, Di Lenarda R, et al. Immunohistochemical identification of decorin and biglycan in human dentin: a correlative field emission scanning electron microscopy/transmission electron microscopy study. Calcif Tissue Int 2007;81:39-45. (Pubitemid 47030204)
21. Orsini G, Ruggeri A Jr, Mazzoni A, Papa V, Piccirilli M, Falconi M, et al. Immunohistochemical identification of type I and type III collagen and chondroitin sulphate in human pre-dentine: a correlative FEI-SEM/TEM study. Int Endod J 2007; 40:669-678
22. Perdigao J, Lambrechts P, Van Meerbeek B, Vanherle G, Lopes AL. Field emission SEM comparison of four postfixation drying techniques for human dentin. J Biomed Mater Res 1995;29:1111-1120
23. Robey PG Vertebrate mineralized matrix proteins: structure and function. Conn Tiss Res 1996; 35:131-136 (Pubitemid 27118843)
24. Robey PG, Fedarko NS, Hefferan TE, Bianco P,Vetter UK, Grzesik W, et al. Structure and molecular regulation of bone matrix proteins. J Bone Min Res 1993;8:483-487 (Pubitemid 24015234)
25. Ruggeri A Jr, Prati C, Mazzoni A, Nucci C, Di Lenarda R, Mazzotti G, et al. Effects of citric acid and EDTA conditioning on exposed root dentin: An immunohistochemical analysis of collagen and proteoglycans. Arch Oral Biol 2007;52:1-8. (Pubitemid 44853918)
26. Sato R, Yamamoto H, Kasai K, Yamauchi M. Distribution pattern of versican, link protein and hyaluronic acid in the rat periodontal ligament during experimental tooth movement. J Periodontal Res 2002; 37:15-22.
27. Septier D, Hall RC, Embery G, Goldberg M. Immunoelectron microscopic visualization of pro- and secreted forms of decorin and biglycan in the predentin and during dentin formation in the rat incisor. Calcif Tissue Int 2002; 69:38-45. (Pubitemid 32702264)
28. Shibata S, Kaneko S,Yanagishita M,Yamashita Y. Histochemical localization of hyaluronan and versican in the rat molar dental pulp. Arch Oral Biol 1999; 44:373-376
29. Shibata S,Yoneda S,Yanagishita M,Yamashita Y .Isolation of proteoglycan (versican) aggregate from rat dental pulp. Arch Oral Biol 2000; 45:563-568
30. Shibata S, Yoneda S, Yanagishita M, Yamashita Y. Developmental changes and regional differences in histochemical localization of hyaluronan and versican in postnatal molar dental pulp. Int Endod J 2002; 35:159-165
31. Shinomura T, Jensen KL,Yamagata M, Kimata K, Solursh M.The distribution of mesenchyme proteoglycan (PG-M) during wing bud outgrowth. Anat and Embryol 1990;181:227-233 (Pubitemid 20153525)
32. Sone S, Nakamura M, Maruya Y,Takahashi I, Mizoguchi I, Mayanagi H, et al. Expression of versican and ADAMTS during rat tooth eruption. J Mol Histol 2005;36:281-288 (Pubitemid 41414405)
33. Suppa P, Ruggeri A Jr,Tay FR, Prati C, Biasotto M, Falconi M, et al. Reduced antigenicity of type I collagen and proteoglycans in sclerotic dentin. J Dent Res 2006; 85:133-137 (Pubitemid 43827744)
34. Teti G, Mazzotti G, Zago M, Ortolani M, Breschi L, Pelotti S, et al. HEMA down-regulates procollagen alpha1 type I in human gingival fibroblasts. J Biomed Mater Res A 2008;90A:256-262.
35. Tsubota M, Sasano Y, Takahashi I, Kagayama M, Shimauchi H. Expression of MMP-8 and MMP-13 mRNAs in rat periodontium during tooth eruption. J Dent Res 2002;81:673-678
36. Waddington RJ, Hall RC, Embery G, Lloyd DM. Changing profiles of proteoglycans in the transition of predentine to dentine. Matrix Biol 2003;22:153-161 (Pubitemid 36627473)
37. Waddington RJ, Moseley R, Smith AJ, Sloan AJ, Embery G. Fluoride-induced changes to proteoglycan structure synthesised within the dentine-pulp complex in vitro. Biochim Biophys Acta 2004;1689: 142-151 (Pubitemid 38766149)
38. Warshawsky H, Moore G. A technique for the fixation and decalcification of rat incisors for electron microscopy. J Histochem Cytochem 1967;15:542-549
39. Yamauchi S, Cheng H, Neame P, Caterson B, Yamauchi M. Identification, partial characterization,and distribution of versican and link protein in bovine dental pulp. J Dent Res 1997;76:1730-1736 (Pubitemid 127732195)
40. Yoshiba N,Yoshiba K, Iwaku M, Ozawa H. Immunolocalization of the small proteoglycan decorin in human teeth. Arch Oral Biol 1996; 41:351-357 (Pubitemid 126652023)
41. Zimmermann DR, Dours-Zimmermann MT, Schubert M, Bruckner-Tuderman L. Versican is expressed in the proliferating zone in the epidermis and in association with the elastic network of the dermis. J Cell Biol 1994;124:817-825 (Pubitemid 24085789)
42. Zimmermann DR, Ruoslahti E. Multiple domains of the large fibroblast proteoglycan, versican. EMBO J 1989;8:2975-2981 (Pubitemid 19274999)