Novel modification of growth medium enables efficient E. coli expression and simple purification of an endotoxin-free recombinant murine Hsp70 protein

Journal of Microbiology and Biotechnology

Volumn 19, Issue 7, 2009, Pages 727-733

  Zachova, Katerina ^a   Krupka, Michal ^a   Chamrad, Ivo ^a   Belakova, Jana ^a   Horynova, Milada ^a   Weigl, Evzen ^a   Sebela, Marek ^a   Raska, Milan ^a  

^a PALACKÝ UNIVERSITY   (Czech Republic)

Author keywords

Affinity purification; Endotoxin removal; GlcNAc; hsp70; MALDI TOF; Protein expression

Indexed keywords

ADENOSINE TRIPHOSPHATASE; ESCHERICHIA COLI ENDOTOXIN; GLUTAMINE FRUCTOSE 6 PHOSPHATE AMINOTRANSFERASE; HEAT SHOCK PROTEIN 70; N ACETYLGLUCOSAMINE; POLYHISTIDINE TAG; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL CELL; BACTERIUM CONTAMINATION; CHEMICAL MODIFICATION; CONCENTRATION (PARAMETERS); CONCENTRATION RESPONSE; CONTROLLED STUDY; CULTURE MEDIUM; DRUG ISOLATION; DRUG PURITY; ENZYME ACTIVITY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION SYSTEM; MOLECULAR WEIGHT; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYSICAL CHEMISTRY; PROCESS DEVELOPMENT; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STABILITY; STORAGE TEMPERATURE;

ACETYLGLUCOSAMINE; ANIMALS; CHROMATOGRAPHY, AFFINITY; CULTURE MEDIA; ENDOTOXINS; ESCHERICHIA COLI; GLUTAMINE-FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING); HSP70 HEAT-SHOCK PROTEINS; INDUSTRIAL MICROBIOLOGY; MICE; RECOMBINANT PROTEINS;

MURINAE;

EID: 70849101168     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.0810.570     Document Type: Article

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