Polarization sensitive, three-dimensional, singlemolecule imaging of cells with a double-helix system

Optics Express

Volumn 17, Issue 22, 2009, Pages 19644-19655

  Pavani, Sri Rama Prasanna ^a   Deluca, Jennifer G ^b   Piestun, Rafael ^a  

^a UCB 450   (United States)

^b Department of Environmental and Radiological Health Sciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIFFRACTION; LIGHT MODULATORS; OPTICAL RESOLVING POWER; OPTICAL TRANSFER FUNCTION;

DOUBLE-HELIX POINT-SPREAD FUNCTIONS; GREEN FLUORESCENT PROTEIN; INTRACELLULAR STRUCTURES; OPTICAL DIFFRACTION LIMIT; ORTHOGONAL POLARIZATIONS; POLARIZATION-SENSITIVE DETECTION; SPATIAL LIGHT MODULATORS; SUPER RESOLUTION IMAGING;

POLARIZATION;

DNA; TUBULIN;

ANIMAL; ARTICLE; CELL CULTURE; COMPUTER AIDED DESIGN; EPITHELIUM CELL; EQUIPMENT; EQUIPMENT DESIGN; EVALUATION; FLUORESCENCE MICROSCOPY; IMAGE ENHANCEMENT; INSTRUMENTATION; POLARIZATION MICROSCOPY; RAT KANGAROO; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; THREE DIMENSIONAL IMAGING; ULTRASTRUCTURE;

ANIMALS; CELLS, CULTURED; COMPUTER-AIDED DESIGN; DNA; EPITHELIAL CELLS; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; IMAGE ENHANCEMENT; IMAGING, THREE-DIMENSIONAL; MICROSCOPY, FLUORESCENCE; MICROSCOPY, POLARIZATION; POTOROIDAE; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TUBULIN;

EID: 70749115711     PISSN: None     EISSN: 10944087     Source Type: Journal    
DOI: 10.1364/OE.17.019644     Document Type: Article

References (24)

1. T. A. Klar, S. Jakobs, M. Dyba, A. Egner, and S. W. Hell, "Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission," Proc. Natl, Acad. Sci. U.S.A. 97(15), 8206-8210 (2000).
2. M. G. L. Gustafsson, "Surpassing the lateral resolution limit by a factor of two using structured .illumination microscopy," J. Microsc. 198(2), 82-87 (2000).
3. E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H, F. Hess, "Imaging intracellular fluorescent proteins at nanometer resolution," Science 313(5793), 1642-1645 (2006).
4. M. J. Rust, M. Bates, and X. Zhuang, "Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)," Nat. Methods 3(10), 793-796 (2006).
5. S. T. Hess, T. P. K. Girirajan, and M. D. Mason, "Ultra-high resolution imaging by fluorescence photoactivation localization microscopy," Biophys. J. 91(11), 4258-4272 (2006).
6. B. Huang, W. Wang, M, Bates, and X. Zhuang, "Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy," Science 319(5864), 810-813 (2008).
7. M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, "Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples," Nat. Methods 5(6), 527-529 (2008).
8. P. Prabhat, S. Ram, E. S. Ward, and R. J. Ober, "Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions," IEEE Trans. Nanobioscience 3(4), 237-242 (2004).
9. A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, "Multilayer three-dimensional super resolution imaging of thick biological samples," Proc. Natl. Acad. Sci. U.S.A. 105(51), 20221-20226 (2008).
10. G. Shtengel, J. A, Galbraith, C. G. Galbraith, J. Lippincott-Schwartz, J. M, Gillette, S. Manley, R. Sougrat, C. M. Waterman, P. Kanchanawong, M. W. Davidson, R. D. Fetter, and H. F. Hess, "Interferometric fluorescent superresolution microscopy resolves 3D cellular ultrastructure," Proc. Natl. Acad. Sci. U.S.A. 106(9), 3125-3130 (2009).
11. S. R. P. Pavani, M, A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R, J. Twieg, R. Piestun, and W. E. Moerner, "Three-dimensional., single-molecule fluorescence imaging beyond the diffraction, limit by using a double-helix point spread function," Proc. Natl. Acad. Sci. U.S.A. 106(9), 2995-2999 (2009).
12. S. R. P. Pavani, and R. Piestun, "High-efficiency rotating point spread functions," Opt. Express 16(5), 34843489 (2008).
13. S. R. P. Pavani, and R. Piestun, "Three dimensional tracking of fluorescent microparticles using a photon-limited double-helix response system," Opt. Express 16(26), 22048-22057 (2008).
14. S. R. P. .Pavani, A. Greengard, and R. Piestun, "Three-dimensional localization with nanometer accuracy using a detector-limited double-helix point spread function system," Appl. Phys. Lett. 95(2), 021103 (2009).
15. T. J. Gould, M. S. Gunewardene, M. V. Gudheti, V. V. Verkhusha, S.-R. Yin, J. A. Gosse, and S. T. Hess, "Nanoscale imaging of molecular positions and anisotropics," Nat. Methods 5(12), 1027-1030 (2008).
16. T. Ha, T. Enderle, D. S. Chemia, and S. Weiss, "Single molecule dynamics studied by polarization modulation," Phys. Rev. Lett. 77(19), 3979-3982 (1996).
17. R. M. Dickson, D. J. Norris, and W. E. Moerner, "Simultaneous Imaging of Individual Molecules Aligned Both Parallel and Perpendicular to the Optic Axis," Phys. Rev. Lett. 81(24), 5322-5325 (1998).
18. B. Sick, B. Hecht, and L. Novotny, "Orientational imaging of single molecules by annular illumination," Phys. Rev. Lett. 85(21), 4482-4485 (2000).
19. M. R. Foreman, C. M. Romero, and P. Török, "Determination of the three-dimensional orientation of single molecules," Opt. Lett. 33(9), 1020-1022 (2008).
20. F. Aguet, S. Geissbühier, I. Märki, T. Lasser, and M. Unser, "Super-resolution orientation estimation and localization of fluorescent dipoles using 3-D steerable filters," Opt. Express 17(8), 6829-6848 (2009).
21. G. H. Patterson, and J. Lippincott-Schwartz, "A photoactivatable GFP for selective photolabeling of proteins and cells," Science 297(5588), 1873-1877 (2002).
22. A. Greengard, Y. Y. Schechner, and R. Piestun, "Depth from diffracted rotation," Opt. Lett. 31(2), 181-183 (2006).
23. S. M, Kay, Fundamentals of Statistical Signal Processing: Estimation Theory (Prentice-Hall, 1993).
24. A. Yariv, and P. Yeh, Photonics: Optical Electronics in Modern Communications (Oxford University Press, New York, 2007).