Human HepG2 cells support respiratory syncytial virus and human metapneumovirus replication

Journal of Virological Methods

Volumn 163, Issue 1, 2010, Pages 74-81

  Schildgen, Verena ^a   Lüsebrink, Jessica ^a   Ditt, Vanessa ^a   Tillmann, Ramona ^a   Simon, Arne ^a   Müller, Andreas ^a   Schildgen, Oliver ^a  

^a UNIVERSITY HOSPITAL BONN   (Germany)

Author keywords

Cell viability assays; HepG2; HMPV; Human cell line; RSV

Indexed keywords

MESSENGER RNA; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; CELL STRAIN HEPG2; CELL VIABILITY; CONTROLLED STUDY; DISEASE MODEL; GENE EXPRESSION REGULATION; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; HUMAN METAPNEUMOVIRUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; UPREGULATION; VIRUS EXPRESSION; VIRUS INFECTION; VIRUS REPLICATION; VIRUS TITRATION;

CELL SURVIVAL; HEP G2 CELLS; HUMANS; METAPNEUMOVIRUS; RESPIRATORY SYNCYTIAL VIRUSES; TUMOR NECROSIS FACTOR-ALPHA; VIRAL LOAD; VIRUS REPLICATION;

ANIMALIA; HUMAN METAPNEUMOVIRUS; HUMAN RESPIRATORY SYNCYTIAL VIRUS; RESPIRATORY SYNCYTIAL VIRUS; RICE STRIPE VIRUS;

EID: 70649114471     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2009.08.024     Document Type: Article

References (39)

1. Alvarez R., and Tripp R.A. The immune response to human metapneumovirus is associated with aberrant immunity and impaired virus clearance in BALB/c mice. J. Virol. 79 (2005) 5971-5978
2. Bao X., Liu T., Spetch L., Kolli D., Garofalo R.P., and Casola A. Airway epithelial cell response to human metapneumovirus infection. Virology 368 (2007) 91-101
3. Bao X., Sinha M., Liu T., Hong C., Luxon B.A., Garofalo R.P., and Casola A. Identification of human metapneumovirus-induced gene networks in airway epithelial cells by microarray analysis. Virology 374 (2008) 114-127
4. Bitko V., Shulyayeva O., Mazumder B., Musiyenko A., Ramaswamy M., Look D.C., and Barik S. Nonstructural proteins of respiratory syncytial virus suppress premature apoptosis by an NF-kappaB-dependent, interferon-independent mechanism and facilitate virus growth. J. Virol. 81 (2007) 1786-1795
5. Burgess J.K. The role of the extracellular matrix and specific growth factors in the regulation of inflammation and remodelling in asthma. Pharmacol Ther (2009)
6. Cseke G., Maginnis M.S., Cox R.G., Tollefson S.J., Podsiad A.B., Wright D.W., Dermody T.S., and Williams J.V. Integrin alphavbeta1 promotes infection by human metapneumovirus. Proc. Natl. Acad. Sci. U.S.A. 106 (2009) 1566-1571
7. Darniot M., Petrella T., Aho S., Pothier P., and Manoha C. Immune response and alteration of pulmonary function after primary human metapneumovirus (hMPV) infection of BALB/c mice. Vaccine 23 (2005) 4473-4480
8. Darniot M., Pitoiset C., Petrella T., Aho S., Pothier P., and Manoha C. Age-associated aggravation of clinical disease after primary metapneumovirus infection of BALB/c mice. J. Virol. (2009)
9. de Graaf M., Osterhaus A.D., Fouchier R.A., and Holmes E.C. Evolutionary dynamics of human and avian metapneumoviruses. J. Gen. Virol. 89 (2008) 2933-2942
10. Deffrasnes C., Cote S., and Boivin G. Analysis of replication kinetics of the human metapneumovirus in different cell lines by real-time PCR. J. Clin. Microbiol. 43 (2005) 488-490
11. Elliott J., Lynch O.T., Suessmuth Y., Qian P., Boyd C.R., Burrows J.F., Buick R., Stevenson N.J., Touzelet O., Gadina M., Power U.F., and Johnston J.A. Respiratory syncytial virus NS1 protein degrades STAT2 by using the Elongin-Cullin E3 ligase. J. Virol. 81 (2007) 3428-3436
12. Hall C.B., Weinberg G.A., Iwane M.K., Blumkin A.K., Edwards K.M., Staat M.A., Auinger P., Griffin M.R., Poehling K.A., Erdman D., Grijalva C.G., Zhu Y., and Szilagyi P. The burden of respiratory syncytial virus infection in young children. N. Engl. J. Med. 360 (2009) 588-598
13. Huck B., Neumann-Haefelin D., Schmitt-Graeff A., Weckmann M., Mattes J., Ehl S., and Falcone V. Human metapneumovirus induces more severe disease and stronger innate immune response in BALB/c mice as compared with respiratory syncytial virus. Respir. Res. 8 (2007) 6
14. Lim X., Bless D.M., Munoz-Del-Rio A., and Welham N.V. Changes in cytokine signaling and extracellular matrix production induced by inflammatory factors in cultured vocal fold fibroblasts. Ann. Otol. Rhinol. Laryngol. 117 (2008) 227-238
15. Ling Z., Tran K.C., and Teng M.N. The human respiratory syncytial virus nonstructural NS2 protein antagonizes the activation of interferon-{beta} transcription by interacting with RIG-I. J. Virol. (2009)
16. Livak K.J., and Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25 (2001) 402-408
17. Lo M.S., Brazas R.M., and Holtzman M.J. Respiratory syncytial virus nonstructural proteins NS1 and NS2 mediate inhibition of Stat2 expression and alpha/beta interferon responsiveness. J. Virol. 79 (2005) 9315-9319
18. Louie J.K., Schnurr D.P., Pan C.Y., Kiang D., Carter C., Tougaw S., Ventura J., Norman A., Belmusto V., Rosenberg J., and Trochet G. A summer outbreak of human metapneumovirus infection in a long-term-care facility. J. Infect. Dis. 196 (2007) 705-708
19. Maertzdorf J., Wang C.K., Brown J.B., Quinto J.D., Chu M., de Graaf M., van den Hoogen B.G., Spaete R., Osterhaus A.D., and Fouchier R.A. Real-time reverse transcriptase PCR assay for detection of human metapneumoviruses from all known genetic lineages. J. Clin. Microbiol. 42 (2004) 981-986
20. Moore E.C., Barber J., and Tripp R.A. Respiratory syncytial virus (RSV) attachment and nonstructural proteins modify the type I interferon response associated with suppressor of cytokine signaling (SOCS) proteins and IFN-stimulated gene-15 (ISG15). Virol. J. 5 (2008) 116
21. Muller A., Kupfer B., Vehreschild J., Cornely O., Kaiser R., Seifert H., Viazov S., Tillmann R.L., Franzen C., Simon A., and Schildgen O. Fatal pneumonia associated with human metapneumovirus (hMPV) in a patient with myeloid leukemia and adenocarcinoma in the lung. Eur. J. Med. Res. 12 (2007) 183-184
22. Munir S., Le Nouen C., Luongo C., Buchholz U.J., Collins P.L., and Bukreyev A. Nonstructural proteins 1 and 2 of respiratory syncytial virus suppress maturation of human dendritic cells. J. Virol. 82 (2008) 8780-8796
23. Ruohola A., Waris M., Allander T., Ziegler T., Heikkinen T., and Ruuskanen O. Viral etiology of common cold in children, Finland. Emerg. Infect. Dis. 15 (2009) 344-346
24. Schildgen O., Glatzel T., Geikowski T., Scheibner B., Matz B., Bindl L., Born M., Viazov S., Wilkesmann A., Knopfle G., Roggendorf M., and Simon A. Human metapneumovirus RNA in encephalitis patient. Emerg. Infect. Dis. 11 (2005) 467-470
25. Schildgen O., Graper S., Blumel J., and Matz B. Genome replication and progeny virion production of herpes simplex virus type 1 mutants with temperature-sensitive lesions in the origin-binding protein. J. Virol. 79 (2005) 7273-7278
26. Schildgen O., Wilkesmann A., and Simon A. Wheezing in patients with human metapneumovirus infection. J. Allergy Clin. Immunol. 117 (2006) 223 (Author reply 223-4)
27. Solly K., Wang X., Xu X., Strulovici B., and Zheng W. Application of real-time cell electronic sensing (RT-CES) technology to cell-based assays. Assay Drug Dev. Technol. 2 (2004) 363-372
28. Spann K.M., Tran K.C., and Collins P.L. Effects of nonstructural proteins NS1 and NS2 of human respiratory syncytial virus on interferon regulatory factor 3, NF-kappaB, and proinflammatory cytokines. J. Virol. 79 (2005) 5353-5362
29. Sprinzl M.F., Oberwinkler H., Schaller H., and Protzer U. Transfer of hepatitis B virus genome by adenovirus vectors into cultured cells and mice: crossing the species barrier. J. Virol. 75 (2001) 5108-5118
30. Teeratakulpisarn J., Ekalaksananan T., Pientong C., and Limwattananon C. Human metapneumovirus and respiratory syncytial virus detection in young children with acute bronchiolitis. Asian Pac. J. Allergy Immunol. 25 (2007) 139-145
31. Tillmann R.L., Simon A., Muller A., and Schildgen O. Sensitive commercial NASBA assay for the detection of respiratory syncytial virus in clinical specimen. PLoS One 2 (2007) e1357
32. van den Hoogen B.G. Respiratory tract infection due to human metapneumovirus among elderly patients. Clin. Infect. Dis. 44 (2007) 1159-1160
33. van den Hoogen B.G., de Jong J.C., Groen J., Kuiken T., de Groot R., Fouchier R.A., and Osterhaus A.D. A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat. Med. 7 (2001) 719-724
34. van den Hoogen B.G., Osterhaus D.M., and Fouchier R.A. Clinical impact and diagnosis of human metapneumovirus infection. Pediatr. Infect. Dis. J. 23 (2004) S25-S32
35. Velayudhan B.T., Nagaraja K.V., Thachil A.J., Shaw D.P., Gray G.C., and Halvorson D.A. Human metapneumovirus in turkey poults. Emerg. Infect. Dis. 12 (2006) 1853-1859
36. von Linstow M.L., Hogh M., Nordbo S.A., Eugen-Olsen J., Koch A., and Hogh B. A community study of clinical traits and risk factors for human metapneumovirus and respiratory syncytial virus infection during the first year of life. Eur. J. Pediatr. 167 (2008) 1125-1133
37. Wilkesmann A., Schildgen O., Eis-Hubinger A.M., Geikowski T., Glatzel T., Lentze M.J., Bode U., and Simon A. Human metapneumovirus infections cause similar symptoms and clinical severity as respiratory syncytial virus infections. Eur. J. Pediatr. 165 (2006) 467-475
38. Williams J.V., Harris P.A., Tollefson S.J., Halburnt-Rush L.L., Pingsterhaus J.M., Edwards K.M., Wright P.F., and Crowe Jr. J.E. Human metapneumovirus and lower respiratory tract disease in otherwise healthy infants and children. N. Engl. J. Med. 350 (2004) 443-450
39. Williams J.V., Tollefson S.J., Heymann P.W., Carper H.T., Patrie J., and Crowe J.E. Human metapneumovirus infection in children hospitalized for wheezing. J. Allergy Clin. Immunol. 115 (2005) 1311-1312