Visualization of multiprotein complexes by flow cytometry

Current Protocols in Immunology

Volumn , Issue SUPPL. 87, 2009, Pages

  Schrum, Adam G ^a  

^a Mayo Clinic   (United States)

Author keywords

Flow cytometry; Immunoprecipitation; Multiprotein complex; Protein protein interactions

Indexed keywords

MULTIPROTEIN COMPLEX;

ARTICLE; CELL LYSATE; CLINICAL PROTOCOL; CONTROLLED STUDY; COVALENT BOND; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; LYMPHOCYTE; LYSIS; MACROPHAGE; METHODOLOGY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PROTEIN INTERACTION; QUANTITATIVE ANALYSIS;

ANIMALS; ANTIBODIES; FLOW CYTOMETRY; HUMANS; IMMUNOPRECIPITATION; INDICATORS AND REAGENTS; MICROSPHERES; MULTIPROTEIN COMPLEXES; PROTEIN INTERACTION MAPPING; PROTEINS;

EID: 70549110905     PISSN: 19343671     EISSN: 1934368X     Source Type: Journal    
DOI: 10.1002/0471142735.im0509s87     Document Type: Article

References (15)

1. Conrotto, P., Yakymovych, I., Yakymovych, M., and Souchelnytskyi, S. 2007. Interactome of transforming growth factor-beta type I receptor (TbetaRI): Inhibition of TGFbeta signaling by Epac1. J. Proteome Res. 6:287-297.
2. Ghavidel, A., Cagney, G., and Emili, A. 2005. A skeleton of the human protein interactome. Cell 122:830-832.
3. Gil, D., Schrum, A.G., Alarcon, B., and Palmer, E. 2005. T cell receptor engagement by peptide- MHC ligands induces a conformational change in the CD3 complex of thymocytes. J. Exp. Med. 201:517-522.
4. Jares-Erijman, E.A. and Jovin, T.M. 2006. Imaging molecular interactions in living cells by FRET microscopy. Curr. Opin. Chem. Biol. 10:409-416.
5. Lund-Johansen, F., Davis, K., Bishop, J., and de Waal Malefyt, R. 2000. Flow cytometric analysis of immunoprecipitates: High-throughput analysis of protein phosphorylation and proteinprotein interactions. Cytometry 39:250-259.
6. Parrish, J.R., Gulyas, K.D., and Finley, R.L. Jr. 2006. Yeast two-hybrid contributions to interactome mapping. Curr. Opin. Biotechnol. 17:387-393.
7. Phizicky, E.M. and Fields, S. 1995. Protein-protein interactions: Methods for detection and analysis. Microbiol. Rev. 59:94-123.
8. Schrum, A.G., Gil, D., Dopfer, E.P., Wiest, D.L., Turka, L.A., Schamel, W.W., and Palmer, E. 2007. High-sensitivity detection and quantitative analysis of native protein-protein interactions and multiprotein complexes by flow cytometry. Sci. STKE Jun 5; 2007(389): Pl2.
9. Smith, D.B. and Johnson, K.S. 1988. Singlestep purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67:31-40.
10. Stelzl, U. and Wanker, E.E. 2006. The value of high quality protein-protein interaction networks for systems biology. Curr. Opin. Chem. Biol. 10:551-558.
11. Swamy, M., Siegers, G.M., Minguet, S., Wollscheid, B., and Schamel, W.W. 2006. Blue native polyacrylamide gel electrophoresis (BNPAGE) for the identification and analysis of multiprotein complexes. Sci. STKE July 25; 2006(345): Pl4.
12. Teixeiro, E., Daniels, M.A., Hausmann, B., Schrum, A.G., Naeher, D., Luescher, I., Thome, M., Bragado, R., and Palmer, E. 2004. T cell division and death are segregated by mutation of TCRbeta chain constant domains. Immunity 21:515-526.
13. Treves, S., Franzini-Armstrong, C., Moccagatta, L., Arnoult, C., Grasso, C., Schrum, A., Ducreux, S., Zhu, M.X., Mikoshiba, K., Girard, T., Smida-Rezgui, S., Ronjat, M., and Zorzato, F. 2004. Junctate is a key element in calcium entry induced by activation of InsP3 receptors and/or calcium store depletion. J. Cell. Biol. 166:537-548.
14. Tu, L.C., Yan, X., Hood, L., and Lin, B. 2007. Proteomics analysis of the interactome of N-myc downstream regulated gene 1 and its interactions with the androgen response program in prostate cancer cells. Mol. Cell Proteomics 6:575-588.
15. Tyers, M. and Mann, M. 2003. From genomics to proteomics. Nature 422:193-197.