Effect of lipid on the conformation of the N-terminal region of equinatoxin II: A synchrotron radiation circular dichroism spectroscopic study

European Biophysics Journal

Volumn 39, Issue 1, 2009, Pages 121-127

  Drechsler, Alison ^a   Miles, Andrew J ^b   Norton, Raymond S ^c   Wallace, B A ^b   Separovic, Frances ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

^b UNIVERSITY OF LONDON   (United Kingdom)

^c WALTER AND ELIZA HALL INSTITUTE OF MEDICAL RESEARCH   (Australia)

Author keywords

Cytolysin; Equinatoxin II; Protein lipid interactions; Secondary structure; Synchrotron radiation circular dichroism (SRCD) spectroscopy

Indexed keywords

CYTOLYSIN; DODECYLPHOSPHORYLCHOLINE; EQUINATOXIN; EQUINATOXIN II; SPHINGOMYELIN; UNCLASSIFIED DRUG; WATER;

ARTICLE; CIRCULAR DICHROISM; MEMBRANE MODEL; MICELLE; NONHUMAN; PEPTIDE ANALYSIS; PROTEIN CONFORMATION; PROTEIN LIPID INTERACTION; PROTEIN SECONDARY STRUCTURE; SPECTROSCOPY; STRUCTURE ANALYSIS; SYNCHROTRON RADIATION; TOXIN ANALYSIS;

AMINO ACID SEQUENCE; CHOLESTEROL; CIRCULAR DICHROISM; CNIDARIAN VENOMS; LIPID BILAYERS; LIPIDS; MICELLES; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PHOSPHATIDYLCHOLINES; PHOSPHORYLCHOLINE; PROTEIN CONFORMATION; SPHINGOMYELINS; SYNCHROTRONS; WATER;

EID: 70450286333     PISSN: 01757571     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00249-009-0445-x     Document Type: Article

References (35)

1. G Anderluh J Pungerčar I Križaj B Strukelj F Gubenšek P Maček 1997 N-Terminal truncation mutagenesis of equinatoxin II, a pore-forming protein from the sea anemone Actinia equina Protein Eng 10 751 755 10.1093/protein/10.7.751 10.1093/protein/10.7.751 1:CAS:528:DyaK2sXms1SmtrY%3D 9342140
2. G Anderluh A Barlič Z Podlesek P Maček J Pungerčar F Gubenšek ML Zecchini M Dalla Serra G Menestrina 1999 Cysteine-scanning mutagenesis of an eukaryotic pore-forming toxin from sea anemone: topology in lipid membranes Eur J Biochem 263 128 136 10.1046/j.1432-1327.1999.00477.x 10.1046/j.1432-1327.1999.00477.x 1:CAS:528:DyaK1MXkslKrsbo%3D 10429196 (Pubitemid 29313847)
3. A Athanasiadis G Anderluh P Maček D Turk 2001 Crystal structure of the soluble form of equinatoxin II, a pore-forming toxin from the sea anemone Actinia equina Structure 9 341 346 10.1016/S0969-2126(01)00592-5 10.1016/S0969-2126(01)00592-5 1:CAS:528:DC%2BD3MXjsValsrk%3D 11525171 (Pubitemid 32772579)
4. G Belmonte C Pederzolli P Maček G Menestrina 1993 Pore formation by the sea anemone cytolysin equinatoxin II in red blood cells and model lipid membranes J Membr Biol 131 11 22 10.1007/BF02258530 10.1007/BF02258530 1:CAS:528:DyaK3sXhtFylur4%3D 7679444 (Pubitemid 23029243)
5. S Brahms J Brahms 1980 Determination of protein secondary structure in solution by vacuum ultraviolet circular dichroism J Mol Biol 138 149 178 10.1016/0022-2836(80)90282-X 10.1016/0022-2836(80)90282-X 1:CAS:528: DyaL3cXitFWgsbg%3D 7411608
6. DT Clarke GR Jones 2004 CD12: a new high-flux beamline for ultraviolet and vacuum-ultraviolet circular dichroism on the SRS, Daresbury J Synchrotron Radiat 11 142 149 10.1107/S0909049503024142 10.1107/S0909049503024142 14960778 (Pubitemid 40087624)
7. LA Compton WC Johnson Jr 1986 Analysis of protein circular dichroism spectra for secondary structure using a simple matrix multiplication Anal Biochem 155 155 167 10.1016/0003-2697(86)90241-1 10.1016/0003-2697(86)90241-1 1:CAS:528:DyaL28XitVegtLc%3D 3717552
8. BA Cornell GC Fletcher J Middlehurst F Separovic 1981 The temperature dependence of the size of phospholipid vesicles Biochim Biophys Acta 642 375 380 10.1016/0005-2736(81)90453-3 10.1016/0005-2736(81)90453-3 1:CAS:528: DyaL3MXhvVOgsLk%3D 7284362
9. BA Cornell GC Fletcher J Middlehurst F Separovic 1982 The lower limit to the size of small sonicated phospholipid vesicles Biochim Biophys Acta 690 15 19 10.1016/0005-2736(82)90233-4 10.1016/0005-2736(82)90233-4 1:CAS:528: DyaL38XlsVSlsrs%3D 7126566
10. BA Cornell LE Weir F Separovic 1988 The effect of gramicidin A on phospholipid bilayers Eur Biophys J 16 113 119 1:CAS:528:DyaL1cXlvVGrt7o%3D 2463153
11. A Drechsler C Potrich JK Sabo M Frisanco G Guella M Dalla Serra G Anderluh F Separovic RS Norton 2006 Structure and activity of the N-terminal region of the eukaryotic cytolysin equinatoxin II Biochemistry 45 1818 1828 10.1021/bi052166o 10.1021/bi052166o 1:CAS:528:DC%2BD28XmvVWkug%3D%3D 16460028 (Pubitemid 43222125)
12. A Filippov G Orädd G Lindblom 2003 The effect of cholesterol on the lateral diffusion of phospholipids in oriented bilayers Biophys J 84 3079 3086 10.1016/S0006-3495(03)70033-2 10.1016/S0006-3495(03)70033-2 1:CAS:528: DC%2BD3sXjvFGjtbk%3D 12719238
13. I Gutiérrez-Aguirre A Barlič Z Podlesek P Maček G Anderluh JM González-Mañas 2004 Membrane insertion of the N-terminal helix of equinatoxin II, a sea anemone cytolytic toxin Biochem J 384 421 428 10.1042/BJ20040601 10.1042/BJ20040601 15317486 (Pubitemid 39656255)
14. MG Hinds W Zhang G Anderluh PE Hansen RS Norton 2002 Solution structure of the eukaryotic pore-forming cytolysin equinatoxin II: implications for pore formation J Mol Biol 315 1219 1229 10.1006/jmbi.2001.5321 10.1006/jmbi.2001.5321 1:CAS:528:DC%2BD38XpsVSkug%3D%3D 11827489 (Pubitemid 34729299)
15. Q Hong I Gutiérrez-Aguirre A Barlič P Malovrh K Kristan Z Podlesek P Maček D Turk J González-Mañas JH Lakey G Anderluh 2002 Two step membrane binding by equinatoxin II, a pore-forming toxin from the sea anemone, involves an exposed aromatic cluster and a flexible helix J Biol Chem 277 41916 41924 10.1074/jbc.M204625200 10.1074/jbc.M204625200 1:CAS:528:DC%2BD38XotF2gsbY%3D 12198118 (Pubitemid 35257504)
16. N Kahya D Scherfeld K Bacia B Poolman P Schwille 2003 Probing lipid mobility of raft-exhibiting model membranes by fluorescence correlation spectroscopy J Biol Chem 278 28109 28115 10.1074/jbc.M302969200 10.1074/jbc.M302969200 1:CAS:528:DC%2BD3sXlsFKrs74%3D 12736276 (Pubitemid 36900008)
17. JG Lees BR Smith F Wien AJ Miles BA Wallace 2004 CDtool-an integrated software package for circular dichroism spectroscopic data processing, analysis and archiving Anal Biochem 332 285 289 10.1016/j.ab.2004.06.002 10.1016/j.ab.2004.06.002 1:CAS:528:DC%2BD2cXmvFeltLg%3D 15325297 (Pubitemid 39485737)
18. JG Lees AJ Miles F Wien BA Wallace 2006 A reference database for circular dichroism spectroscopy covering fold and secondary structure space Bioinformatics 22 1955 1962 10.1093/bioinformatics/btl327 10.1093/ bioinformatics/btl327 1:CAS:528:DC%2BD28Xotl2qtr0%3D 16787970 (Pubitemid 44283007)
19. A Lobley L Whitmore BA Wallace 2002 DICHROWEB: an interactive website for the analysis of protein secondary structure from circular dichroism spectra Bioinformatics 18 211 212 10.1093/bioinformatics/18.1.211 10.1093/ bioinformatics/18.1.211 1:CAS:528:DC%2BD38Xhs1eksrg%3D 11836237
20. P Malovrh G Viero M Dalla Serra Z Podlesek JH Lakey P Maček G Menestrina G Anderluh 2003 A novel mechanism of pore formation J Biol Chem 278 22678 22685 10.1074/jbc.M300622200 10.1074/jbc.M300622200 1:CAS:528: DC%2BD3sXksF2it7k%3D 12676945 (Pubitemid 36830327)
21. P Manavalan WC Johnson Jr 1987 Variable selection method improves the prediction of protein secondary structure from circular dichroism spectra Anal Biochem 167 76 85 10.1016/0003-2697(87)90135-7 10.1016/0003-2697(87)90135-7 1:CAS:528:DyaL1cXjtVai 3434802
22. D Mao BA Wallace 1984 Differential light scattering and absorption flattening optical effects are minimal in the circular dichroism spectra of small unilamellar vesicles Biochemistry 23 2667 2673 10.1021/bi00307a020 10.1021/bi00307a020 1:CAS:528:DyaL2cXitVGqtLw%3D 6466606
23. Marsh D (1990) Handbook of lipid bilayers. CRC Press Inc, Boca Raton
24. AJ Miles F Wien JG Lees A Rodger RW Janes BA Wallace 2003 Calibration and standardisation of synchrotron radiation circular dichroism (SRCD) amplitudes and conventional circular dichroism (CD) spectrophotometers Spectroscopy 17 653 661 1:CAS:528:DC%2BD2cXhsFant7g%3D
25. AJ Miles L Whitmore BA Wallace 2005 Spectral magnitude effects on the analyses of secondary structure from circular dichroism spectroscopic data Protein Sci 14 368 374 10.1110/ps.041019905 10.1110/ps.041019905 1:CAS:528:DC%2BD2MXhtFOrsL8%3D 15659369 (Pubitemid 40194588)
26. AJ Miles A Drechsler K Kristan G Anderluh RS Norton BA Wallace F Separovic 2008 The effects of lipids on the structure of the eukaryotic cytolysin equinatoxin II: a synchrotron radiation circular dichroism spectroscopic study Biochim Biophys Acta 1778 2091 2096 10.1016/j.bbamem.2008. 04.001 10.1016/j.bbamem.2008.04.001 1:CAS:528:DC%2BD1cXhtFCktb%2FK 18440301
27. SW Provencher J Glockner 1981 Estimation of globular protein secondary structure from circular dichroism Biochemistry 20 33 37 10.1021/bi00504a006 10.1021/bi00504a006 1:CAS:528:DyaL3MXis12nsg%3D%3D 7470476
28. P Schön AJ García-Sáez P Malovrh B Bacia G Anderluh P Schwille 2008 Equinatoxin II permeabilizing activity depends on the presence of sphingomyelin and lipid phase coexistence Biophys J 95 691 698 10.1529/biophysj.108.129981 10.1529/biophysj.108.129981 18390598
29. R Smith F Separovic TJ Milne A Whittaker FM Bennett BA Cornell A Makriyannis 1994 Structure and orientation of the pore-forming peptide, melittin, in lipid bilayers J Mol Biol 241 456 466 10.1006/jmbi.1994.1520 10.1006/jmbi.1994.1520 1:CAS:528:DyaK2cXlsFSquro%3D 8064858 (Pubitemid 24266057)
30. N Sreerama RW Woody 1993 A self-consistent method for the analysis of protein secondary structure from circular dichroism Anal Biochem 209 32 44 10.1006/abio.1993.1079 10.1006/abio.1993.1079 1:CAS:528:DyaK3sXht1Shsb8%3D 8465960
31. N Sreerama SY Venyaminov RW Woody 1999 Estimation of the number of α-helical and β-strand segments in proteins using circular dichroism spectroscopy Protein Sci 8 370 380 1:CAS:528:DyaK1MXhtFGlsr8%3D 10048330
32. IHM Van Stokkum HJW Spoelder M Bloemendal R Van Grondelle FCA Groen 1990 Estimation of protein secondary structure and error analysis from circular dichroism spectra Anal Biochem 191 110 118 10.1016/0003-2697(90)90396-Q 10.1016/0003-2697(90)90396-Q 2077933
33. BA Wallace J Lees AJW Orry A Lobley RW Janes 2003 Analyses of circular dichroism spectra of membrane proteins Protein Sci 12 875 884 10.1110/ps.0229603 10.1110/ps.0229603 1:CAS:528:DC%2BD3sXisVekt7w%3D 12649445
34. L Whitmore BA Wallace 2004 DICHROWEB, an online server for protein secondary structure analyses from circular dichroism spectroscopic data Nucleic Acids Res 32 W668 W673 10.1093/nar/gkh371 1:CAS:528:DC%2BD2cXlvFKmsbc%3D 15215473 (Pubitemid 38997420)
35. L Whitmore BA Wallace 2008 Protein secondary structure analyses from circular dichroism spectroscopy: methods and reference databases Biopolymers 89 392 400 10.1002/bip.20853 10.1002/bip.20853 1:CAS:528:DC%2BD1cXksFWqsL4%3D 17896349