Rapid quantification of periodontitis-related bacteria using a novel modification of Invader PLUS technologies

Microbiological Research

Volumn 165, Issue 1, 2010, Pages 43-49

  Tadokoro, Kenichi ^a   Yamaguchi, Toshikazu ^a   Kawamura, Katsumi ^a   Shimizu, Hajime ^a   Egashira, Toru ^a   Minabe, Masato ^b   Yoshino, Toshiaki ^b   Oguchi, Hirokazu ^b  

^a BML   (Japan)

^b The Japanese Academy of Clinical Periodontology   (Japan)

Author keywords

Invader; Periodontitis; Quantification; Real time PCR

Indexed keywords

CLINICAL TESTING; CORRELATION FACTORS; GOOD CORRELATIONS; INTERMEDIA; LINEAR RANGE; PCR AMPLIFICATION; PCR PRIMERS; POLYMERASE CHAIN REACTION; PREVOTELLA; RAPID METHOD; REAL-TIME PCR; TARGET SEQUENCES;

AMPLIFICATION; BACTERIOLOGY; DNA SEQUENCES; NUCLEIC ACIDS;

TECHNOLOGY;

BACTERIAL DNA;

BACTERIUM; DNA; POLYMERASE CHAIN REACTION; REAL TIME;

ARTICLE; BACTERIUM; CHEMISTRY; EVALUATION; GENETIC PROCEDURES; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MICROBIOLOGY; PERIODONTITIS;

BACTERIA; DNA, BACTERIAL; GENETIC TECHNIQUES; HUMANS; PERIODONTITIS;

ACTINOBACILLUS ACTINOMYCETEMCOMITANS; BACTERIA (MICROORGANISMS); FUSOBACTERIUM NUCLEATUM; PORPHYROMONAS GINGIVALIS; PREVOTELLA INTERMEDIA; TANNERELLA FORSYTHENSIS;

EID: 70450223929     PISSN: 09445013     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.micres.2008.06.001     Document Type: Article

References (14)

1. Allawi H.T., Li H., Sander T., Aslanukov A., Lyamichev V.I., Blackman A., et al. Invader plus method detects herpes simplex virus in cerebrospinal fluid and simultaneously differentiates types 1 and 2. J. Clin. Microbiol. 44 (2006) 3443-3447
2. Ashimoto A., Chen C., Bakker I., and Slots J. Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. Oral Microbiol. Immunol. 11 (1996) 266-273
3. Germer J.J., Majewski D.W., Yung B., Mitchell P.S., and Yao J.D. Evaluation of the invader assay for genotyping hepatitis C virus. J. Clin. Microbiol. 44 (2006) 318-323
4. Kofla G., and Ruhnke M. Development of a new real-time TaqMan PCR assay for quantitative analyses of Candida albicans resistance genes expression. J. Microbiol. Methods 68 (2007) 178-183
5. Lau L., Sanz M., Herrera D., Morillow J.M., Martín C., and Silva A. Quantitative real-time polymerase chain reaction versus culture: a comparison between two methods for the detection and quantification of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis and Tannerella forsythensis in subgingival plaque samples. J. Clin. Periodontol. 31 (2004) 1061-1069
6. Luu-The V., Paquet N., Calvo E., and Cumps J. Improved real-time RT-PCR method for high-throughput measurements using second derivative calculation and double correction. Biotechniques 38 (2005) 287-293
7. Lyamichev V., and Neri B. Invader assay for SNP genotyping. Methods Mol. Biol. 212 (2000) 229-2240
8. Maeda H., Fujimoto C., Haruki Y., Maeda T., Kokeguchi S., Petelin M., et al. Quantitative real-time PCR using TaqMan and SYBR Green for Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, tetQ gene and total bacteria. FEMS Immunol. Med. Microbiol. 39 (2003) 81-86
9. Martell M., Gomez J., Esteban J.I., Sauleda S., Quer J., Cabot B., et al. High-throughput real-time reverse transcription-PCR quantitation of hepatitis C virus RNA. J. Clin. Microbiol. 37 (1999) 27-32
10. Martin F.E., Nadkarni M.A., Jacques N.A., and Hunter N. Quantitative microbiological study of human carious dentine by culture and real-time PCR: association of anaerobes with histopathological changes in chronic pulpitis. J. Clin. Microbiol. 40 (2002) 1698-1704
11. Naito M., Matsui A., Inao M., Nagoshi S., Nagano M., Ito N., et al. SNPs in the promoter region of the osteopontin gene as a marker predicting the efficacy of interferon-based therapies in patients with chronic hepatitis C. J. Gastroenterol. 40 (2005) 381-388
12. Nonnenmacher C., Dalpke A., Mutters R., and Heeg K. Quantitative detection of periodontopathogens by real-time PCR. J. Microbiol. Methods 59 (2004) 117-125
13. Sakamoto M., Takeuchi Y., Umeda M., Ishikawa I., and Benno Y. Rapid detection and quantification of five periodontopathic bacteria by real-time PCR. Microbiol. Immunol. 45 (2001) 39-44
14. Yoshida A., Nagashima S., Ansai T., Tachibana M., Kato H., Watari H., et al. Loop-mediated isothermal amplification method for rapid detection of the periodontopathic bacteria Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola. J. Clin. Microbiol. 43 (2005) 2418-2424