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Volumn 131, Issue 45, 2009, Pages 16439-16444

Engineering an inward proton transport from a bacterial sensor rhodopsin

Author keywords

[No Author keywords available]

Indexed keywords

ANABAENA SENSORY RHODOPSINS; ARCHAEAL; BACTERIAL SENSOR; BACTERIORHODOPSIN (BR); CHLORIDE IONS; CYTOPLASMIC DOMAINS; FT-IR SPECTRUM; PROTON AFFINITY; PROTON GRADIENT; PROTON PUMP; PROTON TRANSPORT; PROTON-MOTIVE FORCES; SINGLE PHOTONS;

EID: 70450206030     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja904855g     Document Type: Article
Times cited : (56)

References (31)
  • 15
    • 70450151700 scopus 로고    scopus 로고
    • note
    • The observed inward proton transport can be straightforwardly interpreted as a light-driven inward proton pump. Nevertheless, an inward cation "pump" has to be carefully defined in sphaeroplast vesicles, because the interior of the cell is negatively charged. An ion pump is a protein that transports ions against the electrochemical potential (uphill reaction), whereas the observed inward proton transport may be driven along the potential gradient (downhill reaction). Since the electrochemical potential for protons in the present sphaeroplast vesicles is unclear, we have used the term "inward proton transport" rather than "inward proton pump" in this article. On the other hand, the FTIR results for the ASR sample (Figure 5) are coincident with the proton transport activity, suggesting that the inward proton transport is an intrinsic property of D217E ASR. See footnote 18 for further details.
  • 18
    • 70450178716 scopus 로고    scopus 로고
    • note
    • In the FTIR measurements, detergent-solubilized ASR molecules were reconstituted into PC liposomes. Therefore, there was no electrochemical gradient for the ASR sample, in contrast to the situation for those in sphaeroplast vesicles. Figure 5 clearly shows that protons were transferred to Glu217 under such conditions, which is completely coincident with the proton transport activity of D217E ASR. This observation suggests that D217E ASR is an inward proton pump. However, the active transport has to be measured to obtain direct evidence of an inward proton pump.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.