A photocontrolled molecular switch regulates paralysis in a living organism

Journal of the American Chemical Society

Volumn 131, Issue 44, 2009, Pages 15966-15967

  Al Atar, Usama ^a   Fernandes, Rylan ^a   Johnsen, Bob ^a   Baillie, David ^a   Branda, Neil R ^a  

^a SIMON FRASER UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BIOMEDICAL TECHNOLOGIES; CHEMICAL EQUATIONS; DRUG RELEASE; LIVING ORGANISMS; MOLECULAR SWITCHES; PHOTORESPONSIVE; PHOTOSWITCHES; SIGNIFICANT IMPACTS; STRUCTURAL FORM; TWO DIFFERENT WAVELENGTHS;

CHEMICAL REACTIONS; DRUG THERAPY; PHOTODYNAMIC THERAPY;

BIOLOGY;

DITHIENYLETHENE; ETHYLENE; UNCLASSIFIED DRUG;

ARTICLE; CAENORHABDITIS ELEGANS; DRUG RELEASE; NONHUMAN; PARALYSIS; PHOTOSENSITIVITY;

ANIMALS; CAENORHABDITIS ELEGANS; CYCLIZATION; ETHYLENES; LIGHT; MODELS, ANIMAL; PARALYSIS; PHOTOCHEMOTHERAPY;

EID: 70450198844     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja903070u     Document Type: Article

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17. Each experiment was performed multiple times on different batches of nemotodes: control, 8 studies, 16 plates, 676 worms; 1-c, 3 studies, 10 plates, 313 worms; 1-o, 2 studies, 6 plates, 260 worms.
18. Worms showed differing amounts of uptake and processing of the dye within the first 60 min. See Supporting Information for details.
19. In these studies, three different states of mobility were observed (Figure 1f). After being plated, the nematodes were gently prodded with a small metal wire. Any nematodes that moved rapidly were considered 'mobile', those that showed small movements in parts of their bodies but lacked mobility were considered 'paralyzed', and those that showed no response were considered 'nonresponsive'. In many cases, 'paralyzed' nematodes appeared 'nonresponsive' until prodded.
20. See online Supporting Information for details.