Application of a real-time PCR assay for the detection of Henneguya ictaluri in commercial channel catfish ponds

Diseases of Aquatic Organisms

Volumn 86, Issue 3, 2009, Pages 223-233

  Griffin, Matt J ^a,b   Pote, Linda M ^b   Camus, Alvin C ^c   Mauel, Michael J ^b   Greenway, Terrence E ^a   Wise, David J ^a  

^a Thad Cochran National Warmwater Aquaculture Center   (United States)

^b MISSISSIPPI STATE UNIVERSITY   (United States)

^c UNIVERSITY OF GEORGIA   (United States)

Author keywords

Channel catfish; Henneguya ictaluri; Myxozoa; Proliferative gill disease; Real time PCR

Indexed keywords

BIOASSAY; COMMERCIAL SPECIES; DETECTION METHOD; FISH CULTURE; MORTALITY; PARASITIC DISEASE; POLYMERASE CHAIN REACTION; POND CULTURE; RISK ASSESSMENT;

ANIMAL; ANIMAL DISEASE; ANIMAL PARASITOSIS; AQUACULTURE; ARTICLE; BACTERIAL SPORE; CATFISH; FISH DISEASE; GILL; ISOLATION AND PURIFICATION; MYXOZOA; PARASITOLOGY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ANIMALS; AQUACULTURE; FISH DISEASES; GILLS; ICTALURIDAE; MYXOZOA; PARASITIC DISEASES, ANIMAL; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPORES;

ANNELIDA; HENNEGUYA ICTALURI; ICTALURUS PUNCTATUS; MYXOZOA;

EID: 70450181405     PISSN: 01775103     EISSN: 16161580     Source Type: Journal    
DOI: 10.3354/dao02079     Document Type: Article

References (27)

1. Balacázar JL, Vendrell D, de Blas I, Ruiz-Zarzuela I, Gironés O, Múzquiz JL, (2007).Quantitative detection of Aeromonas salmonicida in fish tissue by real-time PCR using a self quenched fluorogenic probe. Int J Med Microbiol 56: 323-328
2. Bellerud BL, Pote LM, Lin TL, Johnson MJ, Boyle CR (1995) Etiological and epizootological factors associated with outbreaks of proliferative gill disease in channel catfish. J Aquat Anim Health 7:124-131
3. Bilodeau AL, Waldbieser GC, Terhune JS, Wise DJ, Wolters WR, (2003).A real-time polymerase chain reaction assay of the bacterium Edwardsiella ictaluri in channel catfish. J Aquat Anim Health 15:80-86
4. Bowser PR, Conroy JD, (1985).Histopathology of gill lesions in channel catfish associated with Henneguya. J Wildl Dis 21:177-179
5. Brinkman NE, Haughland RA, Wymer LJ, Byappanahalli M, Whitman RL, Vesper SJ, (2003).Evaluation of a rapid quantitative real-time PCR method for enumeration of pathogenic Candida cells in water. Appl Environ Microbiol 69: 1775-1782
6. Burtle GJ, Harrison LR, Styer EL, (1991).Detection of a triactinomyxid myxozoan in an oligochaete from ponds with proliferative gill disease in channel catfish. J Aquat Anim Health 3:281-287
7. Cavender WP, Wood JS, Powell MS, Overturf K, Cain KD (2004) Real-time quantitative polymerase chain reaction (QPCR) to identify Myxobolus cerebralis in rainbow trout Oncorhynchus mykiss. Dis Aquat Org 60:205-213
8. Chase DM, Elliott DG, Pascho RJ, (2006).Detection and quantification of Renibacterium salmoninarum DNA in salmonid tissues by real-time quantitative polymerase chain reaction analysis. J Vet Diagn Invest 18:375-380
9. Elibol-Fleming B, (2006).Effects of Edwardsiella ictaluri infection on transcriptional expression of selected immune relevant genes in channel catfish, Ictalurus punctatus. PhD dissertation, Mississippi State University, Mississippi State, MS
10. Fontaine M, Guillot E, (2003).An immunomagnetic separation real-time PCR method for quantification of Cryptosporidium parvum in water samples. J Microbiol Methods 54: 29-36
11. Griffin MJ, Wise DJ, Camus AC, Mauel MJ, Greenway TE, Pote LM, (2008).A real-time polymerase chain reaction assay for the detection of Henneguya ictaluri in channel catfish. J Vet Diagn Invest 20:559-566
12. Guy RA, Payment P, Krull UJ, Horgen PA, (2003).Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage. Appl Environ Microbiol 69:5178-5185
13. Hallett SL, Bartholomew JL, (2006).Application of a real-time PCR assay to detect and quantify the myxozoan parasite Ceratomyxa shasta in river water samples. Dis Aquat Org 71:109-118
14. Kelley GO, Zaqmutt-Vergara FJ, Leutenegger CM, Myklebust KA and others, (2004).Evaluation of five diagnostic methods for the detection and quantification of Myxobolus cerebralis. J Vet Diagn Invest 16:202-211
15. MacMillan JR, Wilson C, Thiyagarajah A, (1989).Experimental induction of proliferative gill disease in specific-pathogenfree channel catfish. J Aquat Anim Health 1:245-254
16. Panangala VS, Shoemaker CA, Klesius PH, (2007).TaqMan real-time polymerase chain reaction assay for rapid detection of Flavobacterium columnare. Aquacult Res 38: 508-517
17. Pote LM, Bellerud B, Lin DL, Chenney EF, (1994).The isolation and propagation of Dero digitata infected with Aurantiactinomyxon sp. J World Aquacult Soc 25:303-307
18. Pote LM, Hanson LA, Shivaji R, (2000).Small subunit ribosomal RNA sequences link the cause of proliferative gill disease in channel catfish to Henneguya n. sp. (Myxozoa: Myxosporea). J Aquat Anim Health 12:230-240
19. Pote LM, Hanson LA, Khoo L, (2003).Proliferative gill disease. In: Suggested procedures for the detection and identification of certain finfish and shellfish pathogens, Blue Book 5th edn. Fish Health Section, American Fisheries Society, Bethesda, MD
20. Styer EL, Harrison LR, Burtle GJ, (1991).Experimental production of proliferative gill disease in channel catfish exposed to myxozoan-infected oligochaete, Dero digitata. J Aquat Anim Health 3:288-291
21. Thompson K, Nehring RB, (2000).A simple technique used to filter and quantify the actinospore for Myxobolus cerebralis and determine its seasonal abundance in the Colorado river. J Aquat Anim Health 12:316-323
22. Whitaker JW, Pote LM, Khoo L, Shivaji R, Hanson LA (2001) The use of polymerase chain reaction assay to diagnose proliferative gill disease in channel catfish (Ictalurus punctatus). J Vet Diagn Invest 13:394-398
23. Whitaker JW, Pote LM, Hanson LA, (2005).Assay to detect the actinospore and myxospore stages of proliferative gill disease in oligochaetes and pond water. N Am J Aquaculture 67:133-137
24. Wilson IG, (1997).Inhibition and facilitation of nucleic acid amplification. Appl Environ Microbiol 63:3741-3751
25. Wise DJ, Camus A, Schwedler T, Terhune J, (2004).Health management. In: Tucker CS, Hargreaves JA (ed) Biology and culture of the channel catfish. Elsevier, Amsterdam, p 444-502
26. Wise DJ, Griffin MJ, Terhune J, Pote LM, Khoo LH, (2008).Induction and evaluation of proliferative gill disease in channel catfish fingerlings. J Aquat Anim Health 20:236-244
27. Wong ML, Medrano JF, (2005).Real-time PCR for mRNA quantitation. BioTechniques 39:75-85