Can Zeta sperm selection method, recover sperm with higher DNA integrity compare to density gradient centrifugation?

Iranian Journal of Reproductive Medicine

Volumn 7, Issue 2, 2009, Pages 73-77

  Khajavi, Noush Afarin ^a   Razavi, Shahnaz ^a   Mardani, Mohammad ^a   Tavalaee, Marziyeh ^b   Deemeh, Mohammad Reza ^c   Nasr Esfahani, Mohammad Hossein ^b,c  

^a ISFAHAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^b ROYAN INSTITUTE FOR REPRODUCTIVE BIOMEDICINE   (Iran)

^c ISFAHAN FERTILITY AND INFERTILITY CENTER   (Iran)

Author keywords

Density gradient centrifugation method; DNA fragmentation; Zeta method

Indexed keywords

ARTICLE; CELL STRUCTURE; CONTROLLED STUDY; DENSITY GRADIENT CENTRIFUGATION; DNA FRAGMENTATION; DNA INTEGRATION; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; INTRACYTOPLASMIC SPERM INJECTION; MAJOR CLINICAL STUDY; MALE; METHODOLOGY; NICK END LABELING; SEMEN ANALYSIS; SPERMATOZOON MOTILITY;

EID: 70449730775     PISSN: 16806433     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Palermo G, Joris H, Devroey P, Van Steirteghem AC. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 1992; 340: 17-18.
2. Celik-Ozenci C, Jakab A, Kovacs T, Catalanotti J, Demir R, Bray-Ward P, et al. Sperm selection for ICSI: shape properties do not predict the absence or presence of numerical chromosomal aberrations. Hum Reprod 2004; 19: 2052-2059. (Pubitemid 39236472)
3. Razavi S, Nasr-Esfahani MH, Mardani M. The role of sperm chromatin anomalies on the outcome of assisted reproduction techniques. Yakhteh 2006; 28: 206-266.
4. Paasch U, Grunewald S, Glander HJ. Sperm selection in assisted reproductive techniques. Soc Reprod Fertil Suppl 2007; 65: 515-525.
5. Chen MJ, Bongso A. Comparative evaluation of two density gradient preparations for sperm separation for medically assisted conception. Hum Reprod 1999; 14: 759-764. (Pubitemid 29131260)
6. Grunewald S, Miska W, Miska G, Rasch M, Reinhardt M, Glander HJ, et al. Molecular glass wool filtration as a new tool for sperm preparation. Hum Reprod 2007; 22: 1405-1412. (Pubitemid 47062813)
7. Soderlund B, Lundin K. The use of silane-coated silica particles for Density Gradient Centrifugation in in-vitro fertilization. Hum Reprod 2000; 15: 857-860. (Pubitemid 30192735)
8. Razavi S, Nasr-Esfahani MH, Mardani M, Mafi A, Moghdam A. Effect of human sperm chromatin anomalies on fertilization outcome post-ICSI. Andrologia 2003; 35: 238-243. (Pubitemid 37115000)
9. Tavalaee M, Razavi R, Nasr-Esfahani MH. Influence of sperm chromatin anomalies on assisted reproductive technology outcome. Influence of sperm chromatin anomalies on assisted reproductive technology outcome. Fertil Steril 2009; 91: 1119-1126.
10. Nasr-Esfahani MH, Razavi S, Tavalaee M. Evaluation of sperm selection procedure based on hyaluronic acid binding ability on ICSI outcome. Assiste Reprod Genet 2008; 25: 197-203.
11. Ainsworth C, Nixon B, Aitken RJ. Development of a novel electrophoretic system for the isolation of human spermatozoa. Hum Reprod 2005; 20: 2261-2270. (Pubitemid 41418517)
12. Chan PJ, Jacobson JD, Corselli JU, Patton WC . A simple zeta method for sperm selection based on membrane charge. Fertil Steril 2006; 85: 481-486.
13. Said TM, Agarwal A, Zborowski M, Grunewald S, Glander HJ, Paasch U. Utility of magnetic cell separation as a molecular sperm preparation technique. J Androl 2008; 29: 134-142. (Pubitemid 351345710)
14. World Health Organization. WHO laboratory manual for the examination of human semen and semen-cervical mucus interaction. Cambridge: Cambridge University Press, 1999.
15. Sakkas D, Manicardi G.C. The use of the Density Gradient Centrifugation techniques and the swim-up method to separate spermatozoa with chromatin and nuclear DNA anomalies. Hum Reprod 2000; 15: 1112-1116. (Pubitemid 30251310)
16. Zhang HB, Lu SM, Ma CY, Wang L, Li X, Chen ZJ. Early apoptotic changes in human spermatozoa and their relationships with conventional semen parameters and sperm DNA fragmentation. J Androl 2008; 10: 227-235. (Pubitemid 351337984)
17. Bedford JM, Bent MJ, Calvin H. Variations in the structural character and stability of the nuclear chromatin in morphologically normal human spermatozoa. J Reprod Fertile 1973; 18: 199-213.
18. Le Lannou D, Blanchard Y. nuclear maturity and morphology of human spermatozoa selected by percoll density gradient centrifugation or swim-up procedure. J Reprod Fertil 1988; 84: 551-556. (Pubitemid 18268072)
19. Nasr-Esfahani MH, Razavi S, Mardani M. Relation between different human sperm nuclear maturity tests and in vitro fertilization. J Assist Reprod Genet 2001; 18: 219-225. (Pubitemid 32574602)
20. Menkveld R, Rhemrev JP, Franken DR, Vermeiden JP. Acrosomal morphology as a novel criterion for male fertility diagnosis: relation with acrosin activity, morphology (strict criteria), and fertilization in vitro. Fertil Steril 1996; 65: 637-644. (Pubitemid 26069124)
21. Nasr-Esfahani MH, Salehi M, Razavi S, Anjomshoa M, Rozbahani S, Moulavi F, et al. Effect of sperm DNA damage and sperm CMA3 staining on fertilization and embryo development post-ICSI. Reprod Biomed Online 2005; 11: 198-205. (Pubitemid 41086946)