Development of a novel electrophoretic system for the isolation of human spermatozoa

Human Reproduction

Volumn 20, Issue 8, 2005, Pages 2261-2270

  Ainsworth, C ^a   Nixon, B ^a   Aitken, R J ^a,b  

^a UNIVERSITY OF NEWCASTLE   (Australia)

^b UNIVERSITY OF NEWCASTLE   (Australia)

Author keywords

DNA damage; Electrophoretic method; Human spermatozoa; Morphology; Sperm isolation

Indexed keywords

BUFFER; CELL DNA; POLYACRYLAMIDE; POLYCARBONATE; PERCOLL; POVIDONE; SILICON DIOXIDE;

ARTICLE; CELL ISOLATION; CELL STRUCTURE; CELL SUSPENSION; CELL VIABILITY; CENTRIFUGATION; CONCEPTION; CONTAMINATION; CONTROLLED STUDY; DNA DAMAGE; ELECTRIC CURRENT; ELECTRIC POTENTIAL; ELECTROPHORESIS; FILTER; HUMAN; HUMAN CELL; HUMAN EXPERIMENT; INFERTILITY THERAPY; INTERMETHOD COMPARISON; ISOLATION PROCEDURE; LEUKOCYTE; MALE; MEMBRANE BINDING; NORMAL HUMAN; PURIFICATION; SEPARATION TECHNIQUE; SPERMATOZOON; SPERMATOZOON COUNT; SPERMATOZOON MOTILITY; CELL SEPARATION; CELL SURVIVAL; CYTOLOGY; DENSITY GRADIENT CENTRIFUGATION; INSTRUMENTATION; MALE INFERTILITY; METHODOLOGY; NICK END LABELING;

CELL SEPARATION; CELL SURVIVAL; CENTRIFUGATION, DENSITY GRADIENT; DNA DAMAGE; ELECTROPHORESIS; HUMANS; IN SITU NICK-END LABELING; INFERTILITY, MALE; MALE; POVIDONE; REPRODUCTIVE TECHNIQUES, ASSISTED; SILICON DIOXIDE; SPERM MOTILITY; SPERMATOZOA;

EID: 25144504039     PISSN: 02681161     EISSN: 14602350     Source Type: Journal    
DOI: 10.1093/humrep/dei024     Document Type: Article

References (39)

1. Aitken RJ (2004) Fruits of creation, seeds of doubt. Reprod Fertil Dev, 16,655-664.
2. Aitken RJ and Baker HW (1995) Seminal leukocytes: Passengers, terrorists or good samaritans? Hum Reprod 10,1736-1739.
3. Aitken RJ and Clarkson JS (1987) Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. J Reprod Fertil 81,459-469.
4. Aitken RJ and Clarkson JS (1988) Significance of reactive oxygen species and antioxidants in defining the efficacy of sperm preparation techniques. J Androl 9,367-376.
5. Aitken RJ and West KM (1990) Analysis of the relationship between reactive oxygen species production and leucocyte infiltration in fractions of human semen separated on Percoll gradients. Int J Androl 13,433-451.
6. Aitken RJ and Fisher H (1994) Reactive oxygen species generation and human spermatozoa: The balance of benefit and risk. Bioessays 16,259-268.
7. Aitken RJ, Buckingham DW, Brindle J, Gomez E, Baker HW and Irvine DS (1995) Analysis of sperm movement in relation to the oxidative stress created by leukocytes in washed sperm preparations and seminal plasma. Hum Reprod 10,2061-2071.
8. Aitken RJ, Allan IW, Irvine DS and Macnamee M (1996) Studies on the development of diluents for the transportation and storage of human semen at ambient temperature. Hum Reprod 11,2186-2196.
9. Biggers JD, Whitten WK and Whittingham DG (1971) The culture of mouse embryos in vitro. In Daniel JC (ed.) Methods in Mammalian Embryology. Freeman, San Franscisco, CA, USA, pp. 86-116.
10. Braude P, Bolton V and Moore S (1988) Human gene expression first occurs between the four- and eight-cell stages of preimplantation development. Nature 332,459-461.
11. Bungum M, Humaidan P, Spano M, Jepson K, Bungum L and Giwercman A (2004) The predictive value of sperm chromatin structure assay (SCSA) parameters for the outcome of intrauterine insemination, IVF and ICSI. Hum Reprod 19,401-408.
12. Calzada L, Salazar EL and Pedron N (1994) Presence and chemical composition of glycoproteic layer on human spermatozoa. Arch Androl 33, 87-92.
13. Engelmann U, Krassnigg F, Schatz H and Schill WB (1988) Separation of human X and Y spermatozoa by free-flow electrophoresis. Gamete Res 19,151-160.
14. Gandini L, Lombardo F, Paoli D, Caruso F, Eleuteri P, Leter G, Ciriminna R, Culassa F, Dondero F, Lenzi A et al. (2004) Full-term pregnancies achieved with ICSI despite high levels of sperm chromatin damage. Hum Reprod 19,1409-1417.
15. Giuliani V, Pandolfi C, Santucci R, Pelliccione F, Macerola B, Focarelli R, Rosati F, Della Giovampaola C, Francavilla F and Francavilla S (2004) Expression of gp20, a human sperm antigen of epididymal origin, is reduced in spermatozoa from subfertile men. Mol Reprod Dev 69,235-240.
16. Hansen M, Kurinczuk JJ, Bower C and Webb S (2002) The risk of major birth defects after intracytoplasmic sperm injection and in vitro fertilization. N Engl J Med 346,725-730.
17. Kallajoki M, Virtanen I and Suominen J (1986) Surface glycoproteins of human spermatozoa. J Cell Sci 82,11-22.
18. Kirchhoff C and Schroter S (2001) New insights into the origin, structure and role of CD52: A major component of the mammalian sperm glycocalyx. Cells Tissues Organs 168,93-104.
19. Lessley BA and Garner DL (1983) Isolation of motile spermatozoa by density gradient centrifugation in Percoll. Gamete Res 7,49-61.
20. Lewis SEM and Aitken RJ (2005) Sperm DNA damage, fertilization and pregnancy. Cell Tissue Res in press.
21. Lewis SE, Boyle PM, McKinney KA, Young IS and Thompson W (1995) Total antioxidant capacity of seminal plasma is different in fertile and infertile men. Fertil Steril 64,868-870.
22. Lewis SE, Sterling ES, Young IS and Thompson W (1997) Comparison of individual antioxidants of sperm and seminal plasma in fertile and infertile men. Fertil Steril 67,142-147.
23. Loft S, Kold-Jensen T, Hjollund NH, Giwercman A, Gyllemborg J, Ernst E, Olsen J, Scheike T, Poulsen HE and Bonde JP (2003) Oxidative DNA damage in human sperm influences time to pregnancy. Hum Reprod 18,1265-1272.
24. Menkveld R, Stander FSH, Kotze TJvW, Kruger TF and Van Zyl JA (1990) The evaluation of morphological characteristics of human spermatozoa according to strict criteria. Hum Reprod 6,854-859.
25. Mortimer D (1991) Sperm preparation techniques and iatrogenic failures of in vitro fertilization. Hum Reprod 6,173-176.
26. Panidis D, Matalliotakis I, Papathanasiou K, Roussos C and Koumantakis E (1998) The sperm deformity and the multiple anomalies indexes in patients who underwent unilateral orchectomy and preventative radiotherapy. Eur J Obstet Gynecol Reprod Biol 80,247-250.
27. Potts RJ, Notarianni LJ and Jefferies TM (2000) Seminal plasma reduces exogenous oxidative damage to human sperm, determined by the measurement of DNA strand breaks and lipid peroxidation. Mutat Res 447,249-256.
28. Rhemrev JP, van Overveld FW, Haenen GR, Teerlink T, Bast A and Vermeiden JP (2000) Quantification of the nonenzymatic fast and slow TRAP in a postaddition assay in human seminal plasma and the antioxidant contributions of various seminal compounds. J Androl 21,913-920.
29. Saleh RA and Agarwal A (2002) Oxidative stress and male infertility: from research bench to clinical practice. J Androl 23,737-752.
30. Schroter S, Derr P, Conradt HS, Nimtz M, Hale G and Kirchhoff C (1999) Male-specific modification of human CD52. J Biol Chem 274, 29862-29873.
31. Shekarriz M, DeWire DM, Thomas AJ, Jr and Agarwal A (1995) A method of human semen centrifugation to minimize the iatrogenic sperm injuries caused by reactive oxygen species. Eur Urol 28,31-35.
32. Spano M, Bonde JP, Hjollund HI, Kolstad HA, Cordelli E and Leter G (2000) Sperm chromatin damage impairs human fertility. The Danish First Pregnancy Planner Study Team. Fertil Steril 73,43-50.
33. Sun JG, Jurisicova A and Casper RF (1997) Detection of deoxyribonucleic acid fragmentation in human sperm: Correlation with fertilization in vitro. Biol Reprod 56,602-607.
34. Tesarik J, Greco E and Mendoza C (2001) Assisted reproduction with in vitro-cultured testicular spermatozoa in cases of severe germ cell apoptosis: A pilot study. Hum Reprod 16,2640-2645.
35. Twigg JP, Irvine DS and Aitken RJ (1998a) Oxidative damage to DNA in human spermatozoa does not preclude pronucleus formation at ICSI. Hum Reprod 13,1864-1871.
36. Twigg JP, Irvine DS, Houston P, Fulton N, Michael L and Aitken RJ (1998b) Iatrogenic DNA damage induced in human spermatozoa during sperm preparation: Protective significance of seminal plasma. Mol Hum Reprod 4,439-445.
37. Virro MR, Larson-Cook KL and Evenson DP (2004) Sperm chromatin structure assay (SCSA) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection cycles. Fertil Steril 81,1289-1295.
38. World Health Organization (1999) WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction. Cambridge University Press, Cambridge, UK.
39. Zini A, Finelli A, Phang D and Jarvi K (2000) Influence of semen processing technique on human sperm DNA integrity. Urology 56,1081-1084.