Dynamic coupling between actin network flow and turnover revealed by flow mapping in the lamella of crawling fragments

Biochemical and Biophysical Research Communications

Volumn 390, Issue 3, 2009, Pages 797-802

  Okeyo, Kennedy Omondi ^a   Adachi, Taiji ^a,b   Hojo, Masaki ^a  

^a KYOTO UNIVERSITY   (Japan)

^b RIKEN   (Japan)

Author keywords

Actin flow; Actin turnover; Cell migration; Fragments; Mechanobiology; Network assembly; Network disassembly

Indexed keywords

ACTIN;

ACTIN FILAMENT; ANIMAL CELL; ARTICLE; CELL MIGRATION; CORNEA CELL; CYTOSKELETON; MODEL; MOLECULAR DYNAMICS; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN METABOLISM;

ACTINS; ANIMALS; CELL MOVEMENT; FISHES; KERATINOCYTES; MICROFILAMENTS; PSEUDOPODIA;

EID: 70449722760     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2009.10.052     Document Type: Article

References (37)

1. Borisy G.G., and Svitkina T.M. Actin machinery: pushing the envelope. Curr. Opin. Cell Biol. 12 (2000) 104-112
2. Pollard T.D., and Borisy G.G. Cellular motility driven by assembly and disassembly of actin filaments. Cell 112 (2003) 453-465
3. Lauffenburger D.A., and Horwitz A.F. Cell migration: a physically integrated molecular process. Cell 84 (1996) 359-369
4. Li S., Guan J.L., and Chien S. Biochemistry and biomechanics of cell motility. Annu. Rev. Biomed. Eng. 7 (2005) 105-150
5. Fukui Y., Kitanishi-Yumura T., and Yumura S. Myosin Il-independent F-actin flow contributes to cell locomotion in Dictyostelium. J. Cell Sci. 112 (1999) 877-886
6. Cramer L.P. Molecular mechanism of actin-dependent retrograde flow in lamellipodia of motile cells. Front. Biosci. 2 (1997) d260-d270
7. Jurado C., Haserick J.R., and Lee J. Slipping or gripping? Fluorescent speckle microscopy in fish keratocytes reveals two different mechanisms for generating a retrograde flow of actin. Mol. Biol. Cell 16 (2005) 507-518
8. Giannone G., Mège R.-M., and Thoumine O. Multi-level molecular clutches in motile cell processes. Trends Cell Biol. 19 (2009) 475-486
9. Danuser G., and Waterman-Storer C.M. Quantitative fluorescent speckle microscopy of cytoskeleton dynamics. Annu. Rev. Biophys. Biomol. Struct. 35 (2006) 361-387
10. Ji L., Lim J., and Danuser G. Fluctuations of intracellular forces during cell protrusion. Nat. Cell Biol. 10 (2008) 1393-1400
11. Hu K., Ji L., Applegate K.T., Danuser G., and Waterman-Storer C.M. Differential transmission of actin motion within focal adhesions. Science 315 (2007) 111-115
12. Gardel M.L., Sabass B., Ji L., Danuser G., Schwarz U.S., and Waterman C.M. Traction stress in focal adhesions correlates biphasically with actin retrograde flow speed. J. Cell Biol. 183 (2008) 999-1005
13. Heath J., and Holifield B. Actin alone in lamellipodia. Nature 352 (1991) 107-108
14. Willert C.E., and Gharib M. Digital particle image velocimetry. Exp. Fluids 10 (1991) 181-193
15. Verkhovsky A.B., Svitkina T.M., and Borisy G.G. Self-polarization and directional motility of cytoplasm. Curr. Biol. 9 (1999) 11-20
16. Adachi T., Okeyo K.O., Shitagawa Y., and Hojo M. Strain field in actin filament network in lamellipodia of migrating cells: implication for network reorganization. J. Biomech. 42 (2009) 297-302
17. 2+ dependent protein kinase. Biochem. Biophys. Res. Commun. 135 (1986) 397-402
18. Keren K., Pincus Z., Allen G.M., Barnhart E.L., Marriott G., Mogilner A., and Theriot J.A. Mechanism of shape determination in motile cells. Nature 453 (2008) 475-480
19. Svitkina T.M., Verkhovsky A.B., McQuade K.M., and Borisy G.G. Analysis of the actin-myosin II system in fish epidermal keratocytes: mechanism of cell body translocation. J. Cell Biol. 139 (1997) 397-415
20. Salmon W.C., Adams M.C., and Waterman-Storer C.M. Dual-wavelength fluorescent speckle microscopy reveals coupling of microtubule and actin movements in migrating cells. J. Cell Biol. 158 (2002) 31-37
21. Vallotton P., Gupton S.L., Waterman-Storer C.M., and Danuser G. Simultaneous mapping of filamentous actin flow and turnover in migrating cells by quantitative fluorescent speckle microscopy. Proc. Natl. Acad. Sci. USA 101 (2004) 9660-9665
22. Okeyo K.O., Adachi T., Sunaga J., and Hojo M. Actomyosin contractility spatiotemporally regulates actin network dynamics in migrating cells. J. Biomech. 42 (2009) 2540-2548
23. Vallotton P., Danuser G., Bohnet S., Meister J.J., and Verkhovsky A.B. Tracking retrograde flow in keratocytes: news from the front. Mol. Biol. Cell 16 (2005) 1223-1231
24. Lee J., Leonard M., Oliver T., Ishihara A., and Jacobson K. Traction forces generated by locomoting keratocytes. J. Cell Biol. 127 (1994) 1957-1964
25. Oliver T., Dembo M., and Jacobson K. Separation of propulsive and adhesive traction stresses in locomoting keratocytes. J. Cell Biol. 145 (1999) 589-604
26. Watanabe N., and Mitchison T.J. Single-molecule speckle analysis of actin filament turnover in lamellipodia. Science 295 (2002) 1083-1086
27. Henson J.H., Svitkina T.M., Burns A.R., Hughes H.E., MacPartland K.J., Nazarian R., and Borisy G.G. Two components of actin-based retrograde flow in sea urchin coelomocytes. Mol. Biol. Cell 10 (1999) 4075-4090
28. Palecek S.P., Loftus J.C., Ginsberg M.H., Lauffenburger D.A., and Horwitz A.F. Integrin-ligand binding properties govern cell migration speed through cell-substratum adhesiveness. Nature 385 (1997) 537-540
29. Small J.V., Herzog M., and Anderson K. Actin filament organization in the fish keratocyte lamellipodium. J. Cell Biol. 129 (1995) 1275-1286
30. Ponti A., Machacek M., Gupton S.L., Waterman-Storer C.M., and Danuser G. Two distinct actin networks drive the protrusion of migrating cells. Science 305 (2004) 1782-1786
31. Ponti A., Matov A., Adams M., Gupton S., Waterman-Storer C.M., and Danuser G. Periodic patterns of actin turnover in lamellipodia and lamellae of migrating epithelial cells analyzed by quantitative fluorescent speckle microscopy. Biophys. J. 89 (2005) 3456-3469
32. Verkhovsky A.B., Svitkina T.M., and Borisy G.G. Network contraction model for cell translocation and retrograde flow. Biochem. Soc. Symp. 65 (1999) 207-222
33. Ji L., Loerke D., Gardel M., and Danuser G. Probing intracellular force distributions by high-resolution live cell imaging and inverse dynamics. Methods Cell Biol. 83 (2007) 199-235
34. Schaub S., Bohnet S., Laurent V.M., Meister J.J., and Verkhovsky A.B. Comparative maps of motion and assembly of filamentous actin and myosin II in migrating cells. Mol. Biol. Cell 18 (2007) 3723-3732
35. Watanabe N., and Mitchison T.J. Most of the actin filaments in lamellipodia are generated by polymerization away from the lamellipodium tip. Mol. Biol. Cell 12 (2001) 423a
36. Costa K.D., Hucker W.J., and Yin F.C. Buckling of actin stress fibers: a new wrinkle in the cytoskeletal tapestry. Cell Motil. Cytoskeleton 52 (2002) 266-274
37. Chhabra E.S., and Higgs H.N. The many faces of actin: matching assembly factors with cellular structures. Nat. Cell Biol. 9 (2007) 1110-1121