Failure of the BD GeneOhm StaphSR assay for direct detection of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates in positive blood cultures collected in the United States

Journal of Clinical Microbiology

Volumn 47, Issue 11, 2009, Pages 3747-3748

  Snyder, James W ^a   Munier, Gina K ^b   Heckman, Stacy A ^b   Camp, Pamela ^c   Overman, Timothy L ^c,d  

^a University of Louisville School of Medicine   (United States)

^b UNIVERSITY OF LOUISVILLE HOSPITAL   (United States)

^c LEXINGTON VA MEDICAL CENTER   (United States)

^d UNIVERSITY OF KENTUCKY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CEFOXITIN; PENICILLIN BINDING PROTEIN 2A;

ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BLOOD CULTURE; CONTROLLED STUDY; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; NONHUMAN; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCUS AUREUS;

BACTERIOLOGICAL TECHNIQUES; BLOOD; HUMANS; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; MOLECULAR DIAGNOSTIC TECHNIQUES; REAGENT KITS, DIAGNOSTIC; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS; UNITED STATES;

METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; POSIBACTERIA; STAPHYLOCOCCUS AUREUS;

EID: 70449688260     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.01391-09     Document Type: Article

References (8)

1. Bartels, M. D., K. Boye, S. M. Rhode, A. R. Larsen, H. Torfs, P. Bouchy, R. Skov, and H. Westh. 2009. A common variant of staphylococcal cassette chromosome mec type IVa in isolates from Copenhagen, Denmark, is not detected by the BD GeneOhm methicillin-resistant Staphylococcus aureus assay. J. Clin. Microbiol. 47:1524-1527.
2. Cosgrove, S. E., G. Sakoulas, E. N. Perencevich, M. J. Schwaber, A. W. Karchmer, and Y. Carmeli. 2003. Comparison of mortality associated with methicillin-resistant and methicillin-susceptible Staphylococcus aureus bacteremia: a meta-analysis. Clin. Infect. Dis. 36:53-59.
3. Gröbner, S., M. Dion, M. Plante, and V. A. J. Kempf. 2009. Evaluation of the BD GeneOhm StaphSR assay for detection of methicillin- resistant and methicillin-susceptible Staphylococcus aureus isolates from spiked positive blood cultures. J. Clin. Microbiol. 47:1689-1694.
4. Huletsky, A., R. Giroux, V. Rossbach, M. Gagnon, M. Vaillancourt, M. Bernier, F. Gagnon, K. Truchon, M. Bastien, F. J. Picard, A. Van Belkum, M. Ouellette, P. H. Roy, and M. G. Bergeron. 2004. New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci. J. Clin. Microbiol. 42:1875-1884.
5. Stamper, P. D., M. Cai, T. Howard, S. Speser, and K. C. Carroll. 2007. Clinical validation of the molecular BD GeneOhm StaphSR assay for direct detection of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus in positive blood cultures. J. Clin. Microbiol. 45:2191-2196.
6. Thomas, L., M. O'Sullivan, P. Kyme, and J. Iredell. 2008. Failure of the BD GeneOhmS/R assay for identification of Australian methicillin-resistant Staphylococcus aureus strains: duplex assays as the "gold standard" in settings of unknown SCCmec epidemiology. J. Clin. Microbiol. 46:4116-4117.
7. Whitby, M., M. L. McLaws, and G. Berry. 2001. Risk of death from methicillin resistant Staphylococcus aureus bacteraemia: a meta-analysis. Med. J. Aust. 175:264-267.
8. Wolk, D. M., M. J. Sturelens, P. Pancholi, T. Davis, P. Della-Latta, D. Fuller, E. Picton, R. Dickenson, O. Denis, D. Johnson, and K. Chapin. 2009. Rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in wound specimens and blood cultures: multicenter preclinical evaluation of the Cepheid Xpert MRSA/SA skin and soft tissue and blood culture assays. J. Clin. Microbiol. 47:823-826.