Evaluation of the BD GeneOhm StaphSR assay for detection of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from spiked positive blood culture bottles

Journal of Clinical Microbiology

Volumn 47, Issue 6, 2009, Pages 1689-1694

  Gröbner, Sabine ^a   Dion, Mireille ^b   Plante, Mélanie ^b   Kempf, Volkhard A J ^c,d  

^a UNIVERSITY OF TÜBINGEN   (Germany)

^b BD Diagnostics GeneOhm   (Canada)

^c GOETHE UNIVERSITY   (Germany)

^d UNIVERSITY HOSPITAL FRANKFURT   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BIOASSAY; BLOOD CULTURE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; HUMAN; INTERMETHOD COMPARISON; LABORATORY TEST; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; METHICILLIN SUSCEPTIBLE STAPHYLOCOCCUS AUREUS; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STAPHSR ASSAY; STAPHYLOCOCCAL BACTEREMIA; STAPHYLOCOCCUS AUREUS;

BLOOD; HUMANS; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; MOLECULAR DIAGNOSTIC TECHNIQUES; NUCLEIC ACID AMPLIFICATION TECHNIQUES; REAGENT KITS, DIAGNOSTIC; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCUS AUREUS;

METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS AUREUS;

EID: 66749176142     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.02179-08     Document Type: Article

References (21)

1. Chongtrakool, P., T. Ito, X. X. Ma, Y. Kondo, S. Trakulsomboon, C. Tiensasitorn, M. Jamklang, T. Chavalit, J. H. Song, and K. Hiramatsu. 2006. Staphylococcal cassette chromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus strains isolated in 11 Asian countries: a proposal for a new nomenclature for SCCmec elements. Antimicrob. Agents Chemother. 50:1001-1012.
2. CLSI. 2008. Performance standards for antimicrobial susceptibility testing; 18th informational supplement, vol.27. Clinical and Laboratory Standards Institute, Wayne, PA.
3. Cosgrove, S. E. 2006. The relationship between antimicrobial resistance and patient outcomes: mortality, length of hospital stay, and health care costs. Clin. Infect. Dis. 42(Suppl. 2):S82-S89.
4. Cosgrove, S. E., and V. G. Fowler, Jr. 2008. Management of methicillin-resistant Staphylococcus aureus bacteremia. Clin. Infect. Dis. 46(Suppl. 5):S386-S393.
5. Cosgrove, S. E., G. Sakoulas, E. N. Perencevich, M. J. Schwaber, A. W. Karchmer, and Y. Carmeli. 2003. Comparison of mortality associated with methicillin-resistant and methicillin-susceptible Staphylococcus aureus bacteremia: a meta-analysis. Clin. Infect. Dis. 36:53-59.
6. Donnio, P. Y., F. Fevrier, P. Bifani, M. Dehem, C. Kervegant, N. Wilhelm, A. L. Gautier-Lerestif, N. Lafforgue, M. Cormier, and C. A. Le. 2007. Molecular and epidemiological evidence for spread of multiresistant methicillin- susceptible Staphylococcus aureus strains in hospitals. Antimicrob. Agents Chemother. 51:4342-4350.
7. Gröbner, S., and V. A. Kempf. 2007. Rapid detection of methicillin-resistant staphylococci by real-time PCR directly from positive blood culture bottles. Eur. J. Clin. Microbiol. Infect. Dis. 26:751-754.
8. Harmsen, D., H. Claus, W. Witte, J. Rothgänger, H. Claus, D. Turnwald, and U. Vogel. 2003. Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J. Clin. Microbiol. 41:5442-5448.
9. Huletsky, A., R. Giroux, V. Rossbach, M. Gagnon, M. Vaillancourt, M. Bernier, F. Gagnon, K. Truchon, M. Bastien, F. J. Picard, A. van Belkum, M. Ouellette, P. H. Roy, and M. G. Bergeron. 2004. New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci. J. Clin. Microbiol. 42:1875-1884.
10. Kondo, Y., T. Ito, X. X. Ma, S. Watanabe, B. N. Kreiswirth, J. Etienne, and K. Hiramatsu. 2007. Combination of multiplex PCRs for staphylococcal cassette chromosome mec type assignment: rapid identification system for mec, ccr, and major differences in junkyard regions. Antimicrob. Agents Chemother. 51:264-274.
11. Martineau, F., F. J. Picard, N. Lansac, C. Menard, P. H. Roy, M. Ouellette, and M. G. Bergeron. 2000. Correlation between the resistance genotype determined by multiplex PCR assays and the antibiotic susceptibility patterns of Staphylococcus aureus and Staphylococcus epidermidis. Antimicrob. Agents Chemother. 44:231-238. (Pubitemid 30060711)
12. Paule, S. M., A. C. Pasquariello, R. B. Thomson, Jr., K. L. Kaul, and L. R. Peterson. 2005. Real-time PCR can rapidly detect methicillin-susceptible and methicillin-resistant Staphylococcus aureus directly from positive blood culture bottles. Am. J. Clin. Pathol. 124:404-407.
13. Ruimy, R., M. Dos-Santos, L. Raskine, F. Bert, R. Masson, S. Elbaz, C. Bonnal, J. C. Lucet, A. Lefort, B. Fantin, M. Wolff, M. Hornstein, and A. Andremont. 2008. Accuracy and potential usefulness of triplex real-time PCR for improving antibiotic treatment of patients with blood cultures showing clustered gram-positive cocci on direct smears. J. Clin. Microbiol. 46:2045-2051.
14. Small, P. M., and H. F. Chambers. 1990. Vancomycin for Staphylococcus aureus endocarditis in intravenous drug users. Antimicrob. Agents Chemother. 34:1227-1231.
15. Stryjewski, M. E., L. A. Szczech, D. K. Benjamin, Jr., J. K. Inrig, Z. A. Kanafani, J. J. Engemann, V. H. Chu, M. J. Joyce, L. B. Reller, G. R. Corey, and V. G. Fowler, Jr. 2007. Use of vancomycin or first-generation cephalosporins for the treatment of hemodialysis-dependent patients with methicillin- susceptible Staphylococcus aureus bacteremia. Clin. Infect. Dis. 44:190-196.
16. Thomas, L. C., H. F. Gidding, A. N. Ginn, T. Olma, and J. Iredell. 2007. Development of a real-time Staphylococcus aureus and MRSA (SAM-) PCR for routine blood culture. J. Microbiol. Methods 68:296-302.
17. Warren, D. K., R. S. Liao, L. R. Merz, M. Eveland, and W. M. Dunne, Jr. 2004. Detection of methicillin-resistant Staphylococcus aureus directly from nasal swab specimens by a real-time PCR assay. J. Clin. Microbiol. 42:5578-5581.
18. Weinstein, M. P., M. L. Towns, S. M. Quartey, S. Mirrett, L. G. Reimer, G. Parmigiani, and L. B. Reller. 1997. The clinical significance of positive blood cultures in the 1990s: a prospective comprehensive evaluation of the microbiology, epidemiology, and outcome of bacteremia and fungemia in adults. Clin. Infect. Dis. 24:584-602.
19. Wisplinghoff, H., T. Bischoff, S. M. Tallent, H. Seifert, R. P. Wenzel, and M. B. Edmond. 2004. Nosocomial bloodstream infections in US hospitals: analysis of 24,179 cases from a prospective nationwide surveillance study. Clin. Infect. Dis. 39:309-317.
20. Wyllie, D. H., D. W. Crook, and T. E. Peto. 2006. Mortality after Staphylococcus aureus bacteraemia in two hospitals in Oxfordshire, 1997-2003: cohort study. BMJ 333:281.
21. Zhang, K., J. A. McClure, S. Elsayed, T. Louie, and J. M. Conly. 2005. Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 43:5026-5033. (Pubitemid 41483999)