Improved identification of hordeins by cysteine alkylation with 2-bromoethylamine, SDS-PAGE and subsequent in-gel tryptic digestion

Journal of Mass Spectrometry

Volumn 44, Issue 11, 2009, Pages 1613-1621

  Řehulková, Helena ^a,b   Marchetti Deschmann, Martina ^c   Pittenauer, Ernst ^c   Allmaier, Günter ^c   Řehulka, Pavel ^a,b  

^a INSTITUTE OF ANALYTICAL CHEMISTRY   (Czech Republic)

^b UNIVERSITY OF DEFENCE   (Czech Republic)

^c VIENNA UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

2 bromoethylamine (BEA); Cysteine alkylation; Hordeins; In gel digestion; Protein identification

Indexed keywords

ALCOHOL/WATER MIXTURES; AMINO ACID COMPOSITIONS; ARGININE RESIDUE; CHEMICALLY MODIFIED; CLEAVAGE SITES; HIGH-CONTENT; HORDEINS; HORDEUM VULGARE; INGEL DIGESTION; LYSINE RESIDUES; MASS SPECTROMETRIC ANALYSIS; OPTIMAL CONDITIONS; PRIMARY STRUCTURES; PROLINE RESIDUES; PROTEIN IDENTIFICATION; PROTEOMIC PROFILES; SDS-PAGE; SIDE REACTIONS; STORAGE PROTEINS; TERMINAL SIDES; TRYPTIC DIGESTION; 2-BROMOETHYLAMINE (BEA);

ALKYLATION; AMINES; DIFFUSERS (OPTICAL); FLUORINE CONTAINING POLYMERS; GELS; HYDROCARBONS; MASS SPECTROMETERS; ORGANIC ACIDS; PEPTIDES; SPECTROMETRY; ARGININE;

AMINO ACIDS; PEPTIDES;

2 BROMOETHYLAMINE; AMINO ACID; AMINOETHYLCYSTEIN; ARGININE; CYSTEINE; GLUTAMINE; HISTIDINE; HORDEIN; LYSINE; METHIONINE; PEPTIDE; PROLINE; TRYPSIN; UNCLASSIFIED DRUG;

ALKYLATION; AMINO ACID COMPOSITION; ARTICLE; BARLEY; HYDROLYSIS; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CLEAVAGE; PROTEIN STRUCTURE; PROTEOMICS;

ALKYLATION; CYSTEINE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ETHYLAMINES; GLUTENS; HORDEUM; PEPTIDE FRAGMENTS; TRYPSIN;

HORDEUM; HORDEUM VULGARE SUBSP. VULGARE; TRITICUM AESTIVUM;

EID: 70449629788     PISSN: 10765174     EISSN: 10969888     Source Type: Journal    
DOI: 10.1002/jms.1675     Document Type: Article

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