Exhaustive in vivo labelling of plasmid DNA with BrdU for intracellular detection in non-viral transfection of mammalian cells

Biotechnology Journal

Volumn 4, Issue 10, 2009, Pages 1479-1487

  Jérôme, Valérie ^a   Heider, Andreas ^a   Schallon, Anja ^a   Freitag, Ruth ^a  

^a UNIVERSITY OF BAYREUTH   (Germany)

Author keywords

5 Bromodeoxyuridine; DNA detection; Gene delivery; In vivo labelling; Transfection

Indexed keywords

5-BROMODEOXYURIDINE; DNA DETECTION; GENE DELIVERY; IN VIVO LABELLING; TRANSFECTION;

BIOLOGY; BIOSENSORS; DNA; ESCHERICHIA COLI; GENE TRANSFER; GENES; MAMMALS;

NUCLEIC ACIDS;

BROXURIDINE; GREEN FLUORESCENT PROTEIN; PLASMID DNA; POLYCATION; POLYETHYLENEIMINE; PYROXYLIN; THYMIDINE; THYMIDINE DERIVATIVE;

ANIMAL CELL; ARTICLE; BACTERIAL GROWTH; CELL DENSITY; CELLULAR DISTRIBUTION; CHEMICAL LABELING; CHO CELL; CONTROLLED STUDY; CULTURE MEDIUM; CYTOPLASM; DENATURATION; DNA SUPERCOILING; ELECTROPORATION; ESCHERICHIA COLI; FEMALE; FLUORESCENCE MICROSCOPY; GROWTH RATE; IMMUNODETECTION; IN VIVO STUDY; LABELING INDEX; MAMMAL CELL; MEMBRANE BINDING; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PRIORITY JOURNAL; RAT; REPORTER GENE; SUBSTITUTION REACTION;

ANIMALS; BROMODEOXYURIDINE; CELL LINE; CHO CELLS; CRICETINAE; CRICETULUS; DNA; MICROSCOPY, FLUORESCENCE; PLASMIDS; TRANSFECTION;

ESCHERICHIA COLI; MAMMALIA;

EID: 70449580339     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.200900053     Document Type: Article

References (18)

1. Godbey,W.T.,Wu, K. K., Mikos,A. G.,Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery. Proc. Natl. Acad. Sci. USA 1999, 96, 5177-5181.
2. Zabner, J., Fasbender, A. J., Moninger, T., Poellinger, K. A. et al., Cellular and molecular barriers to gene transfer by a cationic lipid. J. Biol. Chem., 1995, 270, 18997-19007.
3. Fong, S., Liu,Y., Heath,T., Fong, P. et al., Membrane-permeant, DNA-binding agents alter intracellular trafficking and increase the transfection efficiency of complexed plasmid DNA. Mol.Ther. 2004, 10, 706-718. (Pubitemid 39272860)
4. Kriss, J. P., Revesz, L., Quantitative studies of incorporation of exogenous thymidine and 5-bromodeoxyuridine into deoxyribonucleic acid of mammalian cells in vitro. Cancer Res. 1961, 21, 1141-1147
5. Traincard, F.,Ternynck,T., Danchin,A.,Avrameas, S.,An immunoenzyme technic for demonstrating the molecular hybridization of nucleic acids. Ann. Immunol. 1983, 134D, 399-404.
6. Dolbeare, F., Bromodeoxyuridine: A diagnostic tool in biology and medicine. Part III. Proliferation in normal, injured and diseased tissue, growth factors, differentiation, DNA replication sites and in situ hybridization. Histochem. J. 1996, 28, 531-575 (Pubitemid 26323225)
7. Leif, R. C., Stein, J. H., Zucker, R. M., A short history of the initial application of anti-5-BrdU to the detection and measurement of S phase. Cytometry A 2004, 58, 45-52.
8. Kitazawa, S., Takenaka, A., Abe, N., Maeda, S. et al., In situ DNA-RNA hybridization using in vivo bromodeoxyuridinelabeled DNA probe. Histochemistry 1989, 92, 195-199. (Pubitemid 19178225)
9. El Ouahabi, A., Thiry, M., Schiffmann, S., Fuks, R. et al., Intracellular visualization of BrdU-labeled plasmid DNA/ cationic liposome complexes. J. Histochem. Cytochem. 1999, 47, 1159-1166. (Pubitemid 29418321)
10. Kanda, T., Sullivan, K. F.,Wahl, G. M., Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells. Curr. Biol. 1998, 8, 377-385. (Pubitemid 28173996)
11. Ueda, J., Saito, H., Watanabe, H., Evers, B. M., Novel and quantitative DNA dot-blotting method for assessment of in vivo proliferation. Am. J. Physiol. Gastrointest. Liver Physiol. 2005, 288, G842-G847.
12. Abramoff, M. D., Magelhaes, P. J., Ram, S. J., Image processing with ImageJ. Biophoton. Int. 2004, 11, 36-42.
13. Fedorov, D., Bioview 3d, Center for BioImage Informatics, UCSB, 2008.
14. Clark-Curtiss, J. E., Curtiss, R. 3rd, Analysis of recombinant DNA using Escherichia coli minicells. Methods Enzymol. 1983, 101, 347-362.
15. Yamamoto,Y.,Yoshisada, F., Uracil-DNA glycosylase causes 5-bromodeoxyuridine photosensitization in Escherichia coli K-12. J. Bacteriol.1990, 172, 5278-5285.
16. Hackett, P. Jr., Hanawalt, P., Selectivity for thymine over 5- bromouracil by a thymine-requiring bacterium. Biochim. Biophys. Acta 1966, 123, 356-363.
17. Tang, M. X., Szoka, F. C.,The influence of polymer structure on the interactions of cationic polymers with DNA and morphology of the resulting complexes. Gene Ther. 1997, 4, 823-832. (Pubitemid 27380165)
18. Köping-Höggard, M.,Tubulekas, I., Guan, H., Edwards, K. et al., Chitosan as a nonviral gene delivery system. Structureproperty relationships and characteristics compared with polyethylenimine in vitro and after lung administration in vivo. Gene Ther. 2001, 8, 1108-1121.