Purification of a bioactive recombinant human Reg IV expressed in Escherichia coli

Protein Expression and Purification

Volumn 69, Issue 2, 2010, Pages 186-190

  Hu, Guoyong ^a   Shen, Jiaqin ^a   Cheng, Li ^a   Xiang, Di ^c   Zhang, Zhonghui ^c   He, Miao ^c   Lu, Huili ^c   Zhu, Shunying ^c   Wu, Mingyuan ^c   Yu, Yan ^c   Wang, Xingpeng ^a,b   Han, Wei ^c  

^a SHANGHAI FIRST PEOPLE S HOSPITAL   (China)

^b TONGJI UNIVERSITY   (China)

^c SHANGHAI JIAO TONG UNIVERSITY   (China)

Author keywords

Cell proliferation; Prokaryotic expression; Protein purification; Regenerating gene IV

Indexed keywords

LECTIN; RECOMBINANT PROTEIN; REG4 PROTEIN, HUMAN;

ANIMAL; ARTICLE; CELL INCLUSION; CELL LINE; CELL PROLIFERATION; CHEMISTRY; ESCHERICHIA COLI; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROTEIN CONFORMATION; PROTEIN FOLDING;

ANIMALS; BASE SEQUENCE; CELL LINE; CELL PROLIFERATION; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ESCHERICHIA COLI; HUMANS; INCLUSION BODIES; LECTINS, C-TYPE; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; PROTEIN FOLDING; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PROKARYOTA;

EID: 70449526098     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.07.018     Document Type: Article

References (21)

1. Hartupee J.C., Zhang H., Bonaldo M.F., Soares M.B., and Dieckgraefe B.K. Isolation and characterization of a cDNA encoding a novel member of the human regenerating protein family: Reg IV. Biochim. Biophys. Acta 1518 (2001) 287-293
2. Zhang Z., and Henzel W.J. Signal peptide prediction based on analysis of experimentally verified cleavage sites. Protein Sci. 13 (2004) 2819-2824
3. Kämäräinen M., Heiskala K., Knuutila S., Heiskala M., Winqvist O., and Andersson L.C. RELP, a novel human REG-like protein with up-regulated expression in inflammatory and metaplastic gastrointestinal mucosa. Am. J. Pathol. 163 (2003) 11-20
4. Violette S., Festor E., Pandrea-Vasile I., Mitchell V., Adida C., Dussaulx E., Lacorte J.M., Chambaz J., Lacasa M., and Lesuffleur T. Reg IV, a new member of the regenerating gene family, is overexpressed in colorectal carcinomas. Int. J. Cancer 103 (2003) 185-193
5. Zhang Y., Lai M., Lv B., Gu X., Wang H., Zhu Y., Zhu Y., Shao L., and Wang G. Overexpression of Reg IV in colorectal adenoma. Cancer Lett. 200 (2003) 69-76
6. Takehara A., Eguchi H., Ohigashi H., Ishikawa O., Kasugai T., Hosokawa M., Katagiri T., Nakamura Y., and Nakagawa H. Novel tumor marker REG4 detected in serum of patients with resectable pancreatic cancer and feasibility for antibody therapy targeting REG4. Cancer Sci. 97 (2006) 1191-1197
7. Miyagawa K., Sakakura C., Nakashima S., Yoshikawa T., Fukuda K., Kin S., Nakase Y., Shimomura K., Oue N., Yasui W., Hayasizaki H., Okazaki Y., Yamagishi H., Hagiwara A., and Otsuji E. Overexpression of Reg IV in peritoneal dissemination of gastric cancer and its potential as a novel marker for the detection of peritoneal micrometastasis. Anticancer Res. 28 (2008) 1169-1179
8. Mitani Y., Oue N., Matsumura S., Yoshida K., Noguchi T., Ito M., Tanaka S., Kuniyasu H., Kamata N., and Yasui W. Reg IV is a serum biomarker for gastric cancer patients and predicts response to 5-fluorouracil-based chemotherapy. Oncogene 26 (2007) 4383-4393
9. Oue N., Mitani Y., Aung P.P., Sakakura C., Takeshima Y., Kaneko M., Noguchi T., Nakayama H., and Yasui W. Expression and localization of Reg IV in human neoplastic and non-neoplastic tissues: Reg IV expression is associated with intestinal and neuroendocrine differentiation in gastric adenocarcinoma. J. Pathol. 207 (2005) 185-198
10. Heiskala K., Giles-Komar J., Heiskala M., and Andersson L.C. High expression of RELP (Reg IV) in neoplastic goblet cells of appendiceal mucinous cystadenoma and pseudomyxoma peritonei. Virchows Arch. 448 (2006) 295-300
11. Nanakin A., Fukui H., Fujii S., Sekikawa A., Kanda N., Hisatsune H., Seno H., Konda Y., Fujimori T., and Chiba T. Expression of the REG IV gene in ulcerative colitis. Lab. Invest. 87 (2007) 304-314
12. Gu Z., Rubin M.A., Yang Y., Deprimo S.E., Zhao H., Horvath S., Brooks J.D., Loda M., and Reiter R.E. Reg IV: a promising marker of hormone refractory metastatic prostate cancer. Clin. Cancer Res. 11 (2005) 2237-2243
13. Ohara S., Oue N., Matsubara A., Mita K., Hasegawa Y., Hayashi T., Usui T., Amatya V.J., Takeshima Y., Kuniyasu H., and Yasui W. Reg IV is an independent prognostic factor for relapse in patients with clinically localized prostate cancer. Cancer Sci. 99 (2008) 1570-1577
14. Li A., Crimmins D.L., Luo Q., Hartupee J., Landt Y., Ladenson J.H., Wilson D., Anant S., and Dieckgraefe B.K. Expression of a novel regenerating gene product, Reg IV, by high density fermentation in Pichia pastoris: production, purification, and characterization. Protein Expr. Purif. 31 (2003) 197-206
15. Bishnupuri K.S., Luo Q., Murmu N., Houchen C.W., Anant S., and Dieckgraefe B.K. Reg IV activates the epidermal growth factor receptor/Akt/AP-1 signaling pathway in colon adenocarcinomas. Gastroenterology 130 (2006) 137-149
16. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
17. Lu H., Yu M., Sun Y., Mao W., Wang Q., Wu M., and Han W. Expression and purification of bioactive high-purity mouse monokine induced by IFN-gamma in Escherichia coli. Protein Expr. Purif. 55 (2007) 132-138
18. Cash H.L., Whitham C.V., and Hooper L.V. Refolding, purification, and characterization of human and murine Reg III protein expressed in Escherichia coli. Protein Expr. Purif. 48 (2006) 151-159
19. Lieu H.T., Batteux F., Simon M.T., Cortes A., Nicco C., Zavala F., Pauloin A., Tralhao J.G., Soubrane O., Weill B., Bréchot C., and Christa L. HIP/PAP accelerates liver regeneration and protects against acetaminophen injury in mice. Hepatology 42 (2005) 618-626
20. Kobayashi S., Akiyama T., Nata K., Abe M., Tajima M., Shervani N.J., Unno M., Matsuno S., Sasaki H., Takasawa S., and Okamoto H. Identification of a receptor for reg (regenerating gene) protein, a pancreatic beta-cell regeneration factor. J. Biol. Chem. 275 (2000) 10723-10726
21. Namikawa K., Fukushima M., Murakami K., Suzuki A., Takasawa S., Okamoto H., and Kiyama H. Expression of Reg/PAP family members during motor nerve regeneration in rat. Biochem. Biophys. Res. Commun. 332 (2005) 126-134