A single amino acid substitution in soybean VSPα increases its acid phosphatase activity nearly 20-fold

Planta

Volumn 219, Issue 6, 2004, Pages 1071-1079

  Leelapon, Oranuch ^a,b   Sarath, Gautam ^a   Staswick, Paul E ^a  

^a UNIVERSITY OF NEBRASKA LINCOLN   (United States)

^b NATIONAL SCIENCE AND TECHNOLOGY DEVELOPMENT AGENCY   (Thailand)

Author keywords

Acid phosphatase; Enzyme activity; Glycine; Mutation; Vegetative storage protein

Indexed keywords

AMINO ACIDS; CATALYSIS; ENZYMES;

PHYLOGENETIC ANALYSIS; PLANT ACID PHOSPHATASES; VEGETATIVE STORAGE PROTEINS;

PLANTS (BOTANY);

ACID PHOSPHATASE; HYBRID PROTEIN; VEGETABLE PROTEIN; VSPA PROTEIN, GLYCINE MAX;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CHEMISTRY; ENZYMOLOGY; GENE EXPRESSION REGULATION; KINETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; SEQUENCE HOMOLOGY; SOYBEAN; STRUCTURE ACTIVITY RELATION;

ACID PHOSPHATASE; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; CONSERVED SEQUENCE; GENE EXPRESSION REGULATION, PLANT; KINETICS; MOLECULAR SEQUENCE DATA; MUTATION; PLANT PROTEINS; RECOMBINANT FUSION PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SOYBEANS; STRUCTURE-ACTIVITY RELATIONSHIP;

AMINO ACIDS; CATALYSTS; ENZYMES; SOY BEANS;

GLYCINE MAX; NEONOTONIA WIGHTII; PHASEOLUS VULGARIS;

EID: 7044263016     PISSN: 00320935     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00425-004-1294-6     Document Type: Article

References (34)

1. Aarts JMMJG, Hontelez GJ, Fischer P, Verkerk R, Kammen A, Zabel P (1991) Acid phosphatase-1, a tightly linked molecular marker for root-knot nematode resistance in tomato: from protein to gene, using PCR and degenerate primers containing deoxyinosine. Plant Mol Biol 16:647-661
2. Andrews DL, Beams B, Summers MD, Park WD (1988) Characterization of the lipid acyl hydrolase activity of the major potato (Solanum tuberosum) tuber protein, patatin, cloning and abundant expression in a baculovirus vector. Biochem J 252:199-206
3. Bewley JD (2002) Root storage proteins, with particular reference to taproots. Can J 80:321-329
4. Chen Y, Rouge P, Peumans WJ, van Damme EJ (2002) Mutational analysis of the carbohydrate-binding activity of the NeuAc(alpha-2,6)Gal/GalNAc-specific type 2 ribosome-inactivating protein from elderberry (Sambucus nigra) fruits. Biochem J 364:587-592
5. Derbyshire E, Writght DJ, Boulter D (1976) Legumin and vicilin storage proteins of legume seeds. Phytochemistry 15:3-24
6. DeWald DB, Mason HS, Mullet JE (1992) The soybean vegetative storage proteins VSPα and VSPβ are acid phosphatases active on polyphosphates. J Biol Chem 267:15658-15964
7. Fiske CH, Subbarow Y (1925) The colorometric detection of phosphorous. J Biol Chem 66:375-400
8. Flores T, Alape-Girón A, Flores-Díaz M, Flores HE (2002) Ocatin. A novel tuber storage protein from the andean tuber crop oca with antibacterial and antifungal activities. Plant Physiol 128:1291-1302
9. Gana JA, Kalengamaliro NE, Cunningham SM, Volenec JJ (1998) Expression of β-amylase from alfalfa taproots. Plant Physiol 118:1495-1506
10. Greenwood JS, Stinissen HM, Peumans WJ, Chrispeels MJ (1986) Sambucus nigra agglutinin is located in proteins bodies in the phloem parenchyma of the bark. Planta 167:275-278
11. Koonin EV, Tatusov RL (1994) Computer analysis of bacterial haloacid dehalogenases defines a large superfamily of hydrolases with diverse specificity. J Mol Biol 244:125-132
12. Liu JQ, Kurihara T, Miyagi M, Esaki N, Soda K (1995) Reaction mechanism of L-2-haloacid dehalogenase of Pseudomonas sp YL. Identification of AsplO as the active site nucleophile by 180 incorporation experiments. J Biol Chem 270:18309-18312
13. Mason HS, Mullet JE (1990) Expression of two vegetative storage protein genes during development and in response to water deficit, wounding and jasmonic acid. Plant Cell 2:569-579
14. Mason HS, DeWald DB, Creelman RA, Mullet JE (1992) Co-regulation of soybean vegetative storage protein gene expression by methyl jasmonate and soluble sugars. Plant Physiol 98:859-867
15. Morais MC, Zhang W, Baker AS, Zhang G, Dunaway-Mariano D, Allen KN (2000) The crystal structure of Bacillus cereus phosphonoacetaldehyde hydrolase: insight into catalysis of phosphorus bond cleavage and catalytic diversification within the HAD enzyme superfamily. Biochemistry 39:10385-10396
16. O'Kennedy BT, Titus JS (1979) Isolation and mobilization of storage proteins from apple shoot bark. Physiol Plant 45:419-424
17. Penheiter AR (1998) Biochemical and genetic characterization of an acid phosphatase/5′-nucleotidase from soybean root nodules. Dissertation, The University of Nebraska-Lincoln
18. Penheiter AR, Duff SMG, Sarath G (1997) Soybean root nodule and acid phosphatase. Plant Physiol 114:597-604
19. Penheiter AR, Klucas RV, Sarath G (1998) Purification and characterization of a soybean root nodule phosphatase expressed in Pichia pastoris. Protein Expr Purif 14:125-130
20. Peumans WJ, Van Damme EJM (1995) Lectins as plant defense proteins. Plant Physiol 109:347-352
21. Sadka A, DeWald DB, May GD, Park WD, Mullet JE (1994) Phosphate modulates transcription of soybean VSPB and other sugar-inducible genes. Plant Cell 6:739-749
22. Selengut J (2001) MDP-1 is a new and distinct member of the haloacid dehalogenase family of aspartate-dependent phosphohydrolases. Biochemistry 40:12704-12711
23. Staswick PE (1994) Storage proteins of vegetative plant tissue. Annu Rev Plant Physiol Plant Mol Biol 45:303-322
24. Staswick PE (1997) The occurrence and gene expression of vegetative storage proteins and a Rubisco Complex Protein in several perennial soybean species. J Exp Bot 48:1-7
25. Staswick PE, Huang J, Rhee Y (1991) Nitrogen and methyl jasmonate induction of soybean vegetative storage protein genes. Plant Physiol 96:130-136
26. Staswick PE, Papa C, Huang JF, Rhee Y (1994) Purification of the major soybean leaf acid phosphatase that is increased by seed-pod removal. Plant Physiol 104:49-57
27. Staswick PE, Zhang Z, Clemente TE, Specht JE (2001) Efficient down regulation of the major vegetative storage protein genes in transgenic soybean does not compromise plant productivity. Plant Physiol 127:1-8
28. Utsugi S, Sakamoto W, Murata M, Motoyoshi F (1998) Arabidopsis thaliana vegetative storage protein (VSP) genes: gene organization and tissue-specific expression. Plant Mol Biol 38:565-576
29. Van Damme EJ, Barre A, Bemer V, Rouge P, Van Leuven F, Peumans WJ (1995) A lectin and a lectin-related protein are the two most prominent proteins in the bark of yellow wood (Cladrastis lutea). Plant Mol Biol 29:579-598
30. Van Damme EJ, Hao Q, Barre A, Rouge P, Van Leuven F, Peumans WJ. (2000) Major protein of resting rhizomes of Calystegia sepium (hedge bindweed) closely resembles plant RNases but has no enzymatic activity. Plant Physiol 122:433-446
31. Williamson VM, Colwell G (1991) Acid phosphatase-1 from nematode resistant tomato. Plant Physiol 70:1544-1548
32. Wittenbach VA (1983) Effect of pod removal on leaf photosynthesis and soluble protein composition of field-grown soybeans. Plant Physiol 73:121-124
33. Yeh KW, Chen JC, Lin MI, Chen YM, Lin CY (1997) Functional activity of sporamin from sweet potato (Ipomoea batatas Lam.): a tuber storage protein with trypsin inhibitory activity. Plant Mol Biol 33:565-570
34. Zhong PY, Tanaka T, Yamauchi D, Minamikawa T (1997) A 28-kilodalton pod storage protein of French bean plants: purification, characterization, and primary structure. Plant Physiol 113:479-485