A novel in vitro stripping method to study geometry of corneocytes with fluorescent microscopy: Example of aging skin

Skin Research and Technology

Volumn 15, Issue 4, 2009, Pages 379-383

  Guz, N V ^a   Gaikwad, R M ^a   Dokukin, M E ^a   Sokolov, I ^a  

^a CLARKSON UNIVERSITY   (United States)

Author keywords

Ageing; Corneocytes geometry; Fluorescent imaging; Stratum corneum; Stripping method

Indexed keywords

AGEING; CORNEOCYTES GEOMETRY; FLUORESCENT IMAGING; STRATUM CORNEUM; STRIPPING METHOD;

COMPUTATIONAL GEOMETRY; DYES; FLUORESCENCE; QUENCHING; SKIN; SOIL STRUCTURE INTERACTIONS;

STRIPPING (DYES);

FLUORESCEIN; RHODAMINE;

ADOLESCENT; ADULT; AGE; AGED; AGING; ARTICLE; CHILD; CONTROLLED STUDY; EPIDERMIS CELL; FLUORESCENCE MICROSCOPY; GEOMETRY; HUMAN; HUMAN CELL; IN VITRO STUDY; MALE; NORMAL HUMAN; STRATUM CORNEUM; VALIDATION STUDY;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; CHILD; DERMOSCOPY; FLUORESCENT DYES; HUMANS; MALE; MICROSCOPY, FLUORESCENCE; MIDDLE AGED; RHODAMINES; SKIN; SKIN AGING; SURGICAL TAPE; YOUNG ADULT;

EID: 70350786311     PISSN: 0909752X     EISSN: 16000846     Source Type: Journal    
DOI: 10.1111/j.1600-0846.2009.00369.x     Document Type: Article

References (13)

1. Grove GL, Kligman AM. Age-associated changes in human epidermal cell renewal. J Gerontol 1983, 38:137-142.
2. Låváque JL, Corcuff P, de Rigal J, Agache P. In vivo studies of the evolution of physical properties of the human skin with age. Int J Dermatol 1984, 23:322-329.
3. Corcuff P, Leveque JL. Corneocyte changes after acute UV irradiation and chronic solar exposure. Photodermatol 1988, 5:110-115.
4. Wolf J. Die innere Struktur der Zellen des Stratum desquamans der menschlichen Epidermis. Z Mikrosk Anat Forsch 1939, 46:170-202.
5. Plewig G, Marples RR. Regional differences of cell sizes in the human stratum corneum. Part I. J Invest Derm 1969, 54:13-18.
6. Corcuff P, Delesalle G, Schaefer H. Quantitative aspect of corneocytes. J Soc Cosmet Chem 1982, 33:1-7.
7. Leeson DT, Meyers CL, Subramanyan K. In vivo confocal fluorescence imaging of skin surface cellular morphology. Int J Cosmet Sci 28:9-20. February 2006
8. Lévêque JL, François G, Sojic N, Giron F. A new technique to in vivo study the corneocyte features at the surface of the skin. Skin Res Technol 2008, 14:1-4.
9. Lévêque JL, Agache PG. Direct observation of size corneocyte. Aging skin: properties and functional 1992, 209-212. In, In, Lévêque JL, ed, New York, Marcel Dekker
10. Corcuff P, Lévêque JL. Corneocyte changes after acute UV irradiation and chronic solar exposure. Photogermatology 1988, 5:110-115.
11. Marks R, Nicholls S, King CS. Studies on isolated corneocytes. Int J Cosmet Sci 1981, 3:251-258.
12. Plewig G. Regional differences of cell sizes in the human stratum corneum. Part II. Effects of sex and age. J Invest Derm 1970, 54:19-23.
13. Grove G, Lavker RM, Holzle E, Kligman AM. Use of non intrusive tests to monitor age associated changes in human skin. J Soc Cosmet Chem 1981, 32:15-26.