End-labeling and analysis of Spo11-oligonucleotide complexes in Saccharomyces cerevisiae

Methods in Molecular Biology

Volumn 557, Issue , 2009, Pages 183-195

  Neale, Matthew J ^b   Keeney, Scott ^a,b  

^a UNIVERSITY OF SUSSEX   (United Kingdom)

^b MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

Author keywords

DNA double strand break; end labeling; meiosis; Spo11; terminal transferase

Indexed keywords

SACCHAROMYCES CEREVISIAE;

DNA TOPOISOMERASE (ATP HYDROLYSING); OLIGONUCLEOTIDE; SACCHAROMYCES CEREVISIAE PROTEIN; SPO11 PROTEIN, S CEREVISIAE;

ALGORITHM; BIOLOGICAL MODEL; CHEMISTRY; DOUBLE STRANDED DNA BREAK; GENETICS; MACROMOLECULE; METABOLISM; METHODOLOGY; NICK END LABELING; PROTEIN BINDING; REVIEW; SACCHAROMYCES CEREVISIAE;

ALGORITHMS; DNA BREAKS, DOUBLE-STRANDED; DNA TOPOISOMERASES, TYPE II, EUKARYOTIC; IN SITU NICK-END LABELING; MACROMOLECULAR SUBSTANCES; MODELS, BIOLOGICAL; OLIGONUCLEOTIDES; PROTEIN BINDING; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 70350754423     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-527-5_12     Document Type: Article

References (8)

1. Keeney, S. (2001) Mechanism and control of meiotic recombination initiation. Curr. Top. Dev. Biol. 52, 1-53.
2. Bergerat, A., de Massy, B., Gadelle, D., Varoutas, P. C., Nicolas, A., and Forterre, P. (1997) An atypical topoisomerase II from Archaea with implications for meiotic recombination. Nature 386, 414-417.
3. Diaz, R. L., Alcid, A. D., Berger, J. M., and Keeney, S. (2002) Identification of residues in yeast Spo11p critical for meiotic DNA double-strand break formation. Mol. Cell. Biol. 22, 1106-1115.
4. Maleki, S., Neale, M. J., Arora, C., Henderson, K. A., and Keeney, S. (2007) Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae. Chromosoma 116, 471-486.
5. Arora, C., Kee, K., Maleki, S., and Keeney, S. (2004) Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break formation, independent of its cytoplasmic role in RNA metabolism. Mol. Cell 13, 549-559.
6. Li, J., Hooker, G. W., and Roeder, G. S. (2006) Saccharomyces cerevisiae Mer2, Mei4 and Rec114 form a complex required for meiotic double-strand break formation. Genetics 173, 1969-1981.
7. Neale, M. J. and Keeney, S. (2006) Clarifying the mechanics of DNA strand exchange in meiotic recombination. Nature 442, 153-158.
8. Neale, M. J., Pan, J., and Keeney, S. (2005) Endonucleolytic processing of covalent protein-linked DNA double-strand breaks. Nature 436, 1053-1057.