Visualization of fluorescence-tagged proteins in fission yeast: The analysis of mitotic spindle dynamics using GFP-tubulin under the native promoter

Methods in Molecular Biology

Volumn 545, Issue , 2009, Pages 185-203

  Sato, Masamitsu ^a   Toya, Mika ^b   Toda, Takashi ^c  

^a UNIVERSITY OF TOKYO   (Japan)

^b RIKEN Center for Biosystems Dynamics Research   (Japan)

^c IMPERIAL CANCER RESEARCH FUND   (United Kingdom)

Author keywords

fission yeast; fluorescence microscopy; Fluorescent protein; gene targeting; spindle microtubule; tubulin

Indexed keywords

SCHIZOSACCHAROMYCES POMBE; SCHIZOSACCHAROMYCETACEAE;

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; SCHIZOSACCHAROMYCES POMBE PROTEIN; TUBULIN;

ARTICLE; CENTROMERE; FLUORESCENCE MICROSCOPY; GENETICS; METABOLISM; MITOSIS SPINDLE; PHYSIOLOGY; PROMOTER REGION; SCHIZOSACCHAROMYCES; ULTRASTRUCTURE;

GREEN FLUORESCENT PROTEINS; KINETOCHORES; MICROSCOPY, FLUORESCENCE; MITOTIC SPINDLE APPARATUS; PROMOTER REGIONS, GENETIC; RECOMBINANT FUSION PROTEINS; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE PROTEINS; TUBULIN;

EID: 70350706744     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-993-2_11     Document Type: Article

References (27)

1. Moreno, S., Klar, A. and Nurse, P. (1991) Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol., 194, 795-823.
2. B̈ahler, J., Wu, J.Q., Longtine, M.S., Shah, N.G., McKenzie, A., 3rd, Steever, A.B., Wach, A., Philippsen, P. and Pringle, J.R. (1998) Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe. Yeast, 14, 943-951. (Pubitemid 28328000)
3. Hentges, P., Van Driessche, B., Tafforeau, L., Vandenhaute, J. and Carr, A.M. (2005) Three novel antibiotic marker cassettes for gene disruption and marker switching in Schizosaccharomyces pombe. Yeast, 22, 1013-1019. (Pubitemid 41582363)
4. Sato, M., Dhut, S. and Toda, T. (2005) New drug-resistant cassettes for gene disruption and epitope tagging in Schizosaccharomyces pombe. Yeast, 22, 583-591. (Pubitemid 40932159)
5. Janke, C., Magiera, M.M., Rathfelder, N., Taxis, C., Reber, S., Maekawa, H., Moreno-Borchart, A., Doenges, G., Schwob, E., Schiebel, E. and Knop, M. (2004) A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes. Yeast, 21, 947-962. (Pubitemid 39206863)
6. Usui, T., Maekawa, H., Pereira, G. and Schiebel, E. (2003) The XMAP215 homologue Stu2 at yeast spindle pole bodies regulates microtubule dynamics and anchorage. EMBO J., 22, 4779-4793. (Pubitemid 37162914)
7. Tanaka, K., Mukae, N., Dewar, H., van Breugel, M., James, E.K., Prescott, A.R., Antony, C. and Tanaka, T.U. (2005) Molecular mechanisms of kinetochore capture by spindle microtubules. Nature, 434, 987-994. (Pubitemid 40586041)
8. Sato, M. and Toda, T. (2007) Alp7/TACC is a crucial target in Ran-GTPase-dependent spindle formation in fission yeast. Nature, 447, 334-337. (Pubitemid 46788834)
9. Grallert, A., Krapp, A., Bagley, S., Simanis, V. and Hagan, I.M. (2004) Recruitment of NIMA kinase shows that maturation of the S. pombe spindle-pole body occurs over consecutive cell cycles and reveals a role for NIMA in modulating SIN activity. Genes Dev., 18, 1007-1021. (Pubitemid 38620241)
10. Radcliffe, P., Hirata, D., Childs, D., Vardy, L. and Toda, T. (1998) Identification of novel temperature-sensitive lethal alleles in essential b-tubulin and nonessential a2-tubulin genes as fission yeast polarity mutants. Mol. Biol. Cell, 9, 1757-1771. (Pubitemid 28337521)
11. Maundrell, K. (1990) nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine. J. Biol. Chem., 265, 10857-10864.
12. Garcia, M.A., Vardy, L., Koonrugsa, N. and Toda, T. (2001) Fission yeast ch-TOG/XMAP215 homologue Alp14 connects mitotic spindles with the kinetochore and is a component of the Mad2-dependent spindle checkpoint. EMBO J., 20, 3389-3401. (Pubitemid 32634347)
13. Bridge, A.J., Morphew, M., Bartlett, R. and Hagan, I.M. (1998) The fission yeast SPB component Cut12 links bipolar spindle formation to mitotic control. Genes Dev., 12, 927-942. (Pubitemid 28204601)
14. Kilmartin, J.V. (2003) Sfi1p has conserved centrin-binding sites and an essential function in budding yeast spindle pole body duplication. J. Cell Biol., 162, 1211-1221. (Pubitemid 37210878)
15. West, R.R., Vaisberg, E.V., Ding, R., Nurse, P. and McIntosh, J.R. (1998) cut11+: A gene required for cell cycle-dependent spindle pole body anchoring in the nuclear envelope and bipolar spindle formation in Schizosaccharomyces pombe. Mol. Biol. Cell, 9, 2839-2855. (Pubitemid 28473664)
16. Saitoh, S., Takahashi, K. and Yanagida, M. (1997) Mis6, a fission yeast inner centromere protein, acts during G1/S and forms specialized chromatin required for equal segregation. Cell, 90, 131-143. (Pubitemid 28009425)
17. Yamamoto, M., Imai, Y. and Watanabe, Y. (1997) Mating and sporulation in Schizosaccharomyces pombe. The Molecular and Cellular Biology of the yeast Saccharomyces, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1037-1106.
18. Yamamoto, A., West, R.R., McIntosh, J.R. and Hiraoka, Y. (1999) A cytoplasmic dynein heavy chain is required for oscillatory nuclear movement of meiotic prophase and efficient meiotic recombination in fission yeast. J. Cell Biol., 145, 1233-1249. (Pubitemid 29293635)
19. Ding, D.Q., Chikashige, Y., Haraguchi, T. and Hiraoka, Y. (1998) Oscillatory nuclear movement in fission yeast meiotic prophase is driven by astral microtubules, as revealed by continuous observation of chromosomes and microtubules in living cells. J Cell Sci, 111, 701-712. (Pubitemid 28163879)
20. Maundrell, K. (1993) Thiamine-repressible expression vectors pREP and pRIP for fission yeast. Gene, 123, 127-130. (Pubitemid 23035804)
21. Tanaka, K. and Kanbe, T. (1986) Mitosis in the fission yeast Schizosaccharomyces pombe as revealed by freeze-substitution electron microscopy. J. Cell Sci., 80, 253-268. (Pubitemid 16139156)
22. Ding, R., McDonald, K.L. and McIntosh, J.R. (1993) Three-dimensional reconstruction and analysis of mitotic spindles from the yeast, Schizosaccharomyces pombe. J. Cell Biol., 120, 141-151.
23. Hagan, I.M. and Hyams, J.S. (1988) The use of cell division cycle mutants to investigate the control of microtubule distribution in the fission yeast Schizosaccharomyces pombe. J. Cell Sci., 89, 343-357.
24. Uzawa, S. and Yanagida, M. (1992) Visualization of centromeric and nucleolar DNA in fission yeast by fluorescence in situ hybridization. J. Cell Sci., 101, 267-275.
25. Mallavarapu, A., Sawin, K. and Mitchison, T. (1999) A switch in microtubule dynamics at the onset of anaphase B in the mitotic spindle of Schizosaccharomyces pombe. Curr. Biol., 9, 1423-1426.
26. Khodjakov, A., La Terra, S. and Chang, F. (2004) Laser microsurgery in fission yeast; role of the mitotic spindle midzone in anaphase B. Curr. Biol., 14, 1330-1340. (Pubitemid 39081634)
27. Drummond, D.R. and Cross, R.A. (2000) Dynamics of interphase microtubules in Schizosaccharomyces pombe. Curr. Biol., 10, 766-775. (Pubitemid 30466939)