Cloning, large-scale production, and purification of active dimeric rat vascular endothelial growth factor (rrVEGF-164)

Protein Expression and Purification

Volumn 69, Issue 1, 2010, Pages 76-82

  Geutjes, Paul J ^a   Nillesen, Suzan T M ^a   Lammers, Gerwen ^a   Daamen, Willeke F ^a   van Kuppevelt, Toin H ^a  

^a RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Angiogenesis; Growth factors; Pichia pastoris; rrVEGF 164

Indexed keywords

COMPLEMENTARY DNA; RECOMBINANT PROTEIN; VASCULOTROPIN A;

AFFINITY CHROMATOGRAPHY; AGAR GEL ELECTROPHORESIS; AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BIOSYNTHESIS; CHEMISTRY; FERMENTATION; GENE VECTOR; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MOLECULAR CLONING; MOLECULAR GENETICS; MOUSE; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN MULTIMERIZATION; RAT; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BLOTTING, WESTERN; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ELECTROPHORESIS, AGAR GEL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FERMENTATION; GENETIC VECTORS; HUMANS; MICE; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; PROTEIN MULTIMERIZATION; RATS; RECOMBINANT PROTEINS; VASCULAR ENDOTHELIAL GROWTH FACTOR A;

EUKARYOTA; PICHIA PASTORIS; RATTUS;

EID: 70350572371     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.08.015     Document Type: Article

References (28)

1. Ferrara N. Role of vascular endothelial growth factor in regulation of physiological angiogenesis. Am. J. Physiol. Cell Physiol. 280 (2001) C1358-C1366
2. Burchardt T., Burchardt M., Chen M.W., Buttyan R., de la T.A., Shabsigh A., and Shabsigh R. Expression of VEGF splice variants 144/145 and 205/206 in adult male tissues. IUBMB Life 48 (1999) 405-408
3. Hara A., Chapin C.J., Ertsey R., and Kitterman J.A. Changes in fetal lung distension alter expression of vascular endothelial growth factor and its isoforms in developing rat lung. Pediatr. Res. 58 (2005) 30-37
4. Zachary I. Vascular endothelial growth factor. Int. J. Biochem. Cell Biol. 30 (1998) 1169-1174
5. Ferrara N. Molecular and biological properties of vascular endothelial growth factor. J. Mol. Med. 77 (1999) 527-543
6. Hoeben A., Landuyt B., Highley M.S., Wildiers H., Van Oosterom A.T., and De Bruijn E.A. Vascular endothelial growth factor and angiogenesis. Pharmacol. Rev. 56 (2004) 549-580
7. Houck K.A., Leung D.W., Rowland A.M., Winer J., and Ferrara N. Dual regulation of vascular endothelial growth factor bioavailability by genetic and proteolytic mechanisms. J. Biol. Chem. 267 (1992) 26031-26037
8. Park J.E., Keller G.A., and Ferrara N. The vascular endothelial growth factor (VEGF) isoforms: differential deposition into the subepithelial extracellular matrix and bioactivity of extracellular matrix-bound VEGF. Mol. Biol. Cell 4 (1993) 1317-1326
9. Gitay-Goren H., Cohen T., Tessler S., Soker S., Gengrinovitch S., Rockwell P., Klagsbrun M., Levi B.Z., and Neufeld G. Selective binding of VEGF121 to one of the three vascular endothelial growth factor receptors of vascular endothelial cells. J. Biol. Chem. 271 (1996) 5519-5523
10. Gerhardt H., and Betsholtz C. Endothelial-pericyte interactions in angiogenesis. Cell Tissue Res. 314 (2003) 15-23
11. Nehls V., Schuchardt E., and Drenckhahn D. The effect of fibroblasts, vascular smooth muscle cells, and pericytes on sprout formation of endothelial cells in a fibrin gel angiogenesis system. Microvasc. Res. 48 (1994) 349-363
12. Pieper J.S., Hafmans T., van Wachem P.B., van Luyn M.J., Brouwer L.A., Veerkamp J.H., and van Kuppevelt T.H. Loading of collagen-heparan sulfate matrices with bFGF promotes angiogenesis and tissue generation in rats. J. Biomed. Mater. Res. 62 (2002) 185-194
13. Shi B.M., Wang X.Y., Mu Q.L., Wu T.H., Liu H.J., and Yang Z. Angiogenesis effect on rat liver after administration of expression vector encoding vascular endothelial growth factor D. World J. Gastroenterol. 9 (2003) 312-315
14. Cregg J.M., Cereghino J.L., Shi J., and Higgins D.R. Recombinant protein expression in Pichia pastoris. Mol. Biotechnol. 16 (2000) 23-52
15. Cereghino G.P.L., Cereghino J.L., Ilgen C., and Cregg J.M. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr. Opin. Biotechnol. 13 (2002) 329-332
16. Ma L., Wang X.N., Zhang Z.Q., Zhou X.M., Zeng G.F., and Chen A.J. Expression, purification and biological activity analysis of human vascular endothelial growth factor (VEGF(165)) in Pichia pastoris. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 33 (2001) 325-330
17. Yu X., Li Z., Xia X., Fang H., Zhou C., and Chen H. Expression and purification of ancrod, an anticoagulant drug, in Pichia pastoris. Protein Expr. Purif. 55 (2007) 257-261
18. Yan Y., Chen J., and Li J. Overexpression of a small medicinal peptide from ginseng in the yeast Pichia pastoris. Protein Expr. Purif. 29 (2003) 161-166
19. Cregg J.M., Barringer K.J., Hessler A.Y., and Madden K.R. Pichia pastoris as a host system for transformations. Mol. Cell. Biol. 5 (1985) 3376-3385
20. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
21. Burnette W.N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. Biochem. 112 (1981) 195-203
22. Lowry O.H., Rosebrough N.J., Farr A.L., and Randall R.J. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193 (1951) 265-275
23. Hamasaki K., Kogure K., and Ohwada K. A biological method for the quantitative measurement of tetrodotoxin (TTX): tissue culture bioassay in combination with a water-soluble tetrazolium salt. Toxicon 34 (1996) 490-495
24. Conn G., Bayne M.L., Soderman D.D., Kwok P.W., Sullivan K.A., Plisi T.M., Hope D.A., and Thomas K.A. Amino acid and cDNA sequences of a vascular endothelial cell mitogen that is homologous to platelet-derived growth factor. Proc. Natl. Acad. Sci. USA 87 (1990) 2628-2632
25. Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F., Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L., Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E., Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C., Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J., Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H., Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W., Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Krzywinski M.I., Skalska U., Smailus D.E., Schnerch A., Schein J.E., Jones S.J., and Marra M.A. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc. Natl. Acad. Sci. USA 99 (2002) 16899-16903
26. Petersen W., Varoga D., Zantop T., Hassenpflug J., Mentlein R., and Pufe T. Cyclic strain influences the expression of the vascular endothelial growth factor (VEGF) and the hypoxia inducible factor 1 alpha (HIF-1alpha) in tendon fibroblasts. J. Orthop. Res. 22 (2004) 847-853
27. Ono K., Hattori H., Takeshita S., Kurita A., and Ishihara M. Structural features in heparin that interact with VEGF165 and modulate its biological activity. Glycobiology 9 (1999) 705-711
28. Siemeister G., Schnurr B., Mohrs K., Schachtele C., Marme D., and Martiny-Baron G. Expression of biologically active isoforms of the tumor angiogenesis factor VEGF in Escherichia coli. Biochem. Biophys. Res. Commun. 222 (1996) 249-255