Studies on recombinant single chain Jacalin lectin reveal reduced affinity for saccharides despite normal folding like native Jacalin

Protein Science

Volumn 13, Issue 12, 2004, Pages 3264-3273

  Sahasrabuddhe, Anagh A ^a   Gaikwad, Sushama M ^b   Krishnasastry, M V ^a   Khan, M Islam ^b  

^a NATIONAL CENTRE FOR CELL SCIENCE   (India)

^b NATIONAL CHEMICAL LABORATORY   (India)

Author keywords

Agglutination activity; Fluorescence studies; Native Jacalin; Recombinant single chain Jacalin; Refolding; Sugar specificity; Thermal unfolding; Thomsen Friedenreich antigen

Indexed keywords

8 ANILINO 1 NAPHTHALENESULFONIC ACID; ACRYLAMIDE; CARBOHYDRATE; CESIUM CHLORIDE; DISACCHARIDE; GALACTOSE; JACALIN; LECTIN; POTASSIUM IODIDE; RECOMBINANT PROTEIN; TRYPTOPHAN;

ARTICLE; BINDING AFFINITY; BREADFRUIT; CIRCULAR DICHROISM; ESCHERICHIA COLI; FLUORESCENCE; HEMAGGLUTINATION; MOLECULAR CLONING; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN GLYCOSYLATION; PROTEIN PROCESSING; PROTEIN RENATURATION; TEMPERATURE;

BASE SEQUENCE; CARBOHYDRATE METABOLISM; CIRCULAR DICHROISM; GLYCOSYLATION; HYDROGEN-ION CONCENTRATION; METHYLGALACTOSIDES; MOLECULAR SEQUENCE DATA; PLANT LECTINS; PROTEIN DENATURATION; PROTEIN FOLDING; RECOMBINANT PROTEINS;

ARTOCARPUS; ARTOCARPUS ALTILIS; ARTOCARPUS HETEROPHYLLUS; ESCHERICHIA COLI;

EID: 9344237665     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.04968804     Document Type: Article

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