Fluorescent lectin assay to quantify particulate marine polysaccharides on 96-well filtration plates

Limnology and Oceanography: Methods

Volumn 7, Issue JUN., 2009, Pages 449-458

  Uthicke, Sven ^a   Llewellyn, Lyndon ^a   Eder, Florian ^a  

^a AUSTRALIAN INSTITUTE OF MARINE SCIENCE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 70350501843     PISSN: 15415856     EISSN: 15415856     Source Type: Journal    
DOI: 10.4319/lom.2009.7.449     Document Type: Article

References (58)

1. Alldredge, A. L., U. Passow, and B. E. Logan. 1993. The abundance and significance of a class of large, transparent organic particles in the ocean. Deep-Sea Res. Part I 40:1131-1140.
2. Alldredge, A. L., and M. W. Silver. 1988. Characteristics, dynamics and significance of marine snow. Prog. Oceanogr. 20:41-82.
3. Benner, R., J. D. Pakulski., M. McCarthy, J. I. Hedges, and P. G. Hatcher. 1992. Bulk chemical characteristics of dissolved organic matter in the ocean. Science 255:1561-1564.
4. Boyd, W. C., and E. Shapleigh. 1954. Antigenic relations of blood group antigens as suggested by tests with lectins. J. Immunol. 73:226-321.
5. Brodie J., G. De'ath, M. Devlin, M. Furnas, and M. Wright. 2007. Spatial and temporal patterns of near-surface chlorophyll a in the Great Barrier Reef lagoon. Mar. Freshw. Res. 58:342-353.
6. Coffroth, M. A. 1990. Mucous sheet formation on poritid corals: an evaluation of coral mucus as a nutrient source on reefs. Mar. Biol. 105:39-49.
7. Cooper T., S. Uthicke, C. Humphrey, and K. Fabricius. 2007. Gradients in water column nutrients, sediments, irradiance and coral reef development in the Whitsunday Region, central Great Barrier Reef. Estuar. Coast. Shelf Sci. 74:458-470.
8. Crocker, K. M., and U. Passow. 1995. Differential aggregation of diatoms. Mar. Ecol. Prog. Ser. 117:249-257.
9. Decho, A. W. 2000. Microbial biofilms in intertidal systems: an overview. Cont. Shelf Res. 20:1257-1273.
10. Dubois, M., K. A. Gilles, J. K. Hamilton, P. A. Rebers, and F. Smith. 1956. A colorimetric determination of polysaccharides and related substances. Anal. Chem. 28:350-356.
11. Ducklow, H. W., and R. Mitchell. 1979. Composition of mucus released by coral reef coelenterates. Limnol. Oceanogr. 24:706-714.
12. Eisma, D. 1986. Flocculation and de-flocculation of suspended matter in estuaries. Neth. J. Sea Res. 20:183-199.
13. Engel, A. 2000. The role of transparent exopolymer particles (TEP) in the increase in apparent particle stickiness (alpha) during the decline of a diatom bloom. J. Plankton Res. 22:485-497.
14. Fabricius, K. E., C. Wild, E. Wolanski, and D. Abele. 2003. Effects of transparent exopolymer particles and muddy terrigenous sediments on the survival of hard coral recruits. Estuar. Coast. Shelf Sci. 57:613-621.
15. Fabricius, K. E., and E. Wolanski. 2000. Rapid smothering of coral reef organisms by muddy marine snow. Estuar. Coast. Shelf Sci. 50:115-120.
16. Farina, R. D., and R. G. Wilkins. 1980. Kinetics of interaction of some alpha- and beta-D-monosaccharides with concanavalin A. Biochim. Biophys. Acta 428-38.
17. Gabius, H. J., and S. Gabius, 1997. Glycosciences, status and perspectives. Chapman and Hall, Weinheim.
18. Goto, N., T. Kawamura, O. Mitamura, and H. Terai. 1999. Importance of extracellular organic carbon production in the total primary production by tidal-flat diatoms in comparison to phytoplankton. Mar. Ecol. Prog. Ser. 190:289-295.
19. Hama, T., and N. Handa. 1983. The seasonal variation of organic constituents in a eutrophic lake, Lake Suwa, Japan. Part II. Dissolved organic matter. Arch. Hydrobiol. 98:443-462.
20. Haug, A., and S. Myklestad. 1976. Polysaccharides of marine diatoms with special reference to Chaetoceros species. Mar. Biol. 34:217-222.
21. Hoagland, K. D., J. R. Rosowski, M. R. Gretz, and S. C. Roemer. 1993. Diatom extracellular polymeric substances: function, fine structure, chemistry, and physiology. J. Phycol. 29:537-565.
22. Huettel, M., C. Wild, and S. Gonelli. 2006. Mucus trap in coral reefs: formation and temporal evolution of particle aggregates caused by coral mucus. Mar. Ecol. Prog. Ser. 307:69.
23. Hung, C., L. Guo, P. H. Santschi, N. Alvarado-Quiroz, and J. M. Haye. 2003. Distributions of carbohydrate species in the Gulf of Mexico. Mar. Chem. 81:119-135.
24. Johannes, R. E. 1967. Ecology of organic aggregates in the vicinity of a coral reef. Limnol. Oceanogr. 12:189-195.
25. Lev, R., and S. S. Spicer. 1964. Specific staining of sulphate groups with Alcian blue at low pH. J. Histochem. Cytochem. 12:309.
26. Lis, H., and N. Sharon. 1998. Lectins: carbohydrate-specific proteins that mediate cellular recognition. Chemical Rev. 98:637-674.
27. Long, R. A., and F. Azam. 2001. Microscale patchiness of bacterioplankton assemblage richness in seawater. Aquat. Microb. Ecol. 26:103-113.
28. Loris, R., T. Hamelryck, J. Bouckaert, and L. Wyns. 1998. Legume lectin structure. Biochim. Biophys. Acta 1383:9-36.
29. Mari, X., S. Beauvais, R. Lemee, and M. L. Pedrotti. 2001. Non-Redfield C:N ratio of transparent exopolymeric particles in the northwestern Mediterranean Sea. Limnol. Oceanogr. 46:1831-1836.
30. Mari, X., and T. Kiorboe. 1996. Abundance, size distribution and bacterial colonization of transparent exopolymeric particles (TEP) during spring in the Kattegat. J. Plankton Res. 18:969-986.
31. McCave, I. N. 1984. Size spectra and aggregation of suspended particles in the deep ocean. Deep-Sea Res. 31:329-352.
32. McKee, M. P., J. E. Ward, B. A. MacDonald, and B. A. Holohan. 2005. Production of transparent exopolymer particles (TEP) by the eastern oyster Crassostrea virginica. Mar. Ecol. Prog. Ser. 288:141-149.
33. Mopper, K., J. Zhou, K. Sri Ramana, U. Passow, H. G. Dam, and D. T. Drapeau, 1995. The role of surface-active carbohydrates in the flocculation of a diatom bloom in a mesocosm. Deep-Sea Res. Part II 42:47-73.
34. Myklestad, S. 1995. Release of extracellular products by phytoplankton with special emphasis on polysaccharides. Sci. Total Environ. 165:155-164.
35. Neu, T. R., G. D. W. Swerhone, and J. R. Lawrence. 2001. Assessment of lectin-binding analysis for in situ detection of glycoconjugates in biofilm systems. Microbiology 147:299-313.
36. Neu, T. R. 2000. In situ cell and glycoconjugate distribution in river snow studied by confocal laser scanning microscopy. Aquat. Microb. Ecol. 21:85-95.
37. Neu, T. R., G. D. W. Swerhone, U. Boeckelmann, J. R. Lawrence. 2005. Effect of CNP on composition and structure of lotic biofilms as detected with lectin-specific glycoconjugates. Aquat. Microb. Ecol. 38:283-294.
38. Passow, U. 2000. Formation of transparent exopolymer particles, TEP, from dissolved precursor material. Mar. Ecol. Prog. Ser. 192:1-11.
39. Passow, U. 2002a. Production of transparent exopolymer particles (TEP) by phyto- and bacterioplankton. Mar. Ecol. Prog. Ser. 236:1-12.
40. Passow, U. 2002b. Transparent exopolymer particles (TEP) in aquatic environments. Prog. Oceanogr. 55:287-333.
41. Passow, U., and A. L. Alldredge. 1995. A dye-binding assay for the spectrophotometric measurement of transparent exopolymer particles (TEP). Limnol. Oceanogr. 40:1326-1335.
42. Ramaiah, N., T. Yoshikawa, and K. Furuya. 2001. Temporal variations in transparent exopolymer particles (TEP) associated with a diatom spring bloom in a subarctic ria in Japan. Mar. Ecol. Prog. Ser. 212:79-88.
43. Ramus, J. 1977. Alcian blue: A quantitative aqueous assay for algal acid and sulfated polysaccharides. J. Phycol. 13:345-348.
44. Simon, M., H. P. Grossart, B. Schweitzer, and H. Ploug, 2002. Microbial ecology of organic aggregates in aquatic ecosystems. Aquat. Microb. Ecol. 28:175-211.
45. Staats, N., B. De Winder, L. J. Stal, and L. R. Mur, 1999. Isolation and characterization of extracellular polysaccharides from the epipelic diatoms Cylindrotheca closterium and Navicula salinarum. Eur. J. Phycol. 34:161-169.
46. Steedman, H. F. 1950. Alcian blue 8GS: a new stain for mucin. Q. J. Microsc Sci. 91:477-479.
47. Stoderegger, K. E., and G. J. Herndl. 1999. Production of exopolymer particles by marine bacterioplankton under contrasting turbulence conditions. Mar. Ecol. Prog. Ser. 189:9-16.
48. Thornton, D. C. O. 2004. Formation of transparent exopolymeric particles (TEP) from macroalgal detritus. Mar. Ecol. Prog. Ser. 282:1-12.
49. Thornton, D. C. O., E.M. Fejes, S. F. DiMarco, and K. M. Clancy. 2007. Measurement of acid polysaccharides in marine and freshwater samples using Alcian blue. Limnol. Oceanogr. Methods 5:73-87.
50. Underwood, G. J., M. Boulcott, C. A. Raines, and K. Waldron. 2004. Environmental effects on exopolymer production by marine benthic diatoms: dynamics, changes in composition, and pathways of production. J. Phycol. 40:293-304.
51. Uthicke, S. 2006. Photosynthetic efficiency and rapid light curves of sediment-biofilms along a water quality gradient in the Great Barrier Reef, Australia. Mar. Ecol. Prog. Ser. 322:61-73.
52. Uthicke, S., and K. Nobes. 2008. Benthic foraminifera as indicators for terrestrial runoff: a foram index for the GBR. Estuar. Coast. Shelf Sci. 78:763-773.
53. Van Damme, E. J. M., W. J. Peumans, A. Pusztai, and S. Bardocz. 1998. Handbook of plant lectins: properties and biochemical applications. John Wiley and Sons, New York.
54. Vrolijk, N. H., N. H. Target, R. E. Baier, and A. E. Mayer. 1990. Surface characterization of two gorgonian coral species: implications for a natural antifouling defence. Biofouling 2:39-54.
55. Waite, A. M., R. J. Olson, H. G. Dam, and U. Passow. 1995. Sugar-containing compounds on the cell surfaces of marine diatoms measured using concanavalin A and flow cytometry. J. Phycol. 31:925-933.
56. Wigglesworth-Cooksey, B., and K. E. Cooksey. 1992. Can diatoms sense surfaces? State of our knowledge. Biofouling 5:227-238.
57. Wingender, J., T. R. Neu, and H.-C. Flemming. 1999. Microbial extracellular polymeric substances. Springer, New York.
58. Zhou, J., K. Mopper, and U. Passow. 1998. The role of surfaceactive carbohydrates in the formation of transparent exopolymer particles by bubble adsorption of seawater. Limnol. Oceanogr. 43:1860-1871.