A quantitative reverse transcription-PCR assay for rapid, automated analysis of breast cancer sentinel lymph nodes

Journal of Molecular Diagnostics

Volumn 11, Issue 6, 2009, Pages 576-582

  Hughes, Steven J ^a   Xi, Liqiang ^b   Gooding, William E ^c   Cole, David J ^d   Mitas, Michael ^d   Metcalf, John ^d   Bhargava, Rohit ^a   Dabbs, David ^a   Ching, Jesus ^e   Kozma, Lynn ^e   McMillan, William ^e   Godfrey, Tony E ^a  

^a UNIVERSITY OF PITTSBURGH MEDICAL CENTER   (United States)

^b MOUNT SINAI SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF PITTSBURGH CANCER INSTITUTE   (United States)

^d MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

^e CEPHEID   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACCURACY; ARTICLE; AUTOMATION; BREAST CANCER; CONTROLLED STUDY; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; MAJOR CLINICAL STUDY; METASTASIS; POINT OF CARE TESTING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SENTINEL LYMPH NODE;

EID: 70350440657     PISSN: 15251578     EISSN: None     Source Type: Journal    
DOI: 10.2353/jmoldx.2009.090037     Document Type: Article

References (33)

1. Fisher B, Bauer M, Wickerham DL, Redmond CK, Fisher ER, Cruz AB, Foster R, Gardner B, Lerner H, Margolese R: Relation of number of positive axillary nodes to the prognosis of patients with primary breast cancer. an NSABP update. Cancer 1983, 52:1551-1557
2. Fisher ER, Sass R, Fisher B: Pathologic findings from the National Surgical Adjuvant Project for Breast Cancers (protocol no. 4). X. Discriminants for tenth year treatment failure. Cancer 1984, 53:712-723
3. Fisher ER, Costantino J, Fisher B, Redmond C: Pathologic findings from the National Surgical Adjuvant Breast Project (protocol 4). Discriminants for 15-year survival. National Surgical Adjuvant Breast and Bowel Project Investigators. Cancer 1993, 71:2141-2150
4. Cote RJ, Peterson HF, Chaiwun B, Gelber RD, Goldhirsch A, Castiglione- Gertsch M, Gusterson B, Neville AM: Role of immunohistochemical detection of lymph-node metastases in management of breast cancer. Lancet 1999, 354:896-900 (Pubitemid 29424019)
5. Giuliano AE, Dale PS, Turner RR, Morton DL, Evans SW, Krasne DL: Improved axillary staging of breast cancer with sentinel lymphadenectomy. Ann Surg 1995, 222:394-399
6. Sakorafas GH, Geraghty J, Pavlakis G: The clinical significance of axillary lymph node micrometastases in breast cancer. Eur J Surg Oncol 2004, 30:807-816 (Pubitemid 39186065)
7. Chen SL, Hoehne FM, Giuliano AE: The prognostic significance of micrometastases in breast cancer: a SEER population-based analysis. Ann Surg Oncol 2007, 14:3378-3384 (Pubitemid 350160103)
8. Rutgers EJ: Sentinel node biopsy: interpretation and management of patients with immunohistochemistry-positive sentinel nodes and those with micrometastases. J Clin Oncol 2008, 26:698-702
9. Kuijt GP, Voogd AC, van de Poll-Franse LV, Scheijmans LJ, van Beek MW, Roumen RM: The prognostic significance of axillary lymph-node micrometastases in breast cancer patients. Eur J Surg Oncol 2005, 31:500-505 (Pubitemid 40724091)
10. Dabbs DJ, Fung M, Johnson R: Intraoperative cytologic examination of breast sentinel lymph nodes: test utility and patient impact. Breast J 2004, 10:190-194 (Pubitemid 38715733)
11. Chao C, Wong SL, Ackermann D, Simpson D, Carter MB, Brown CM, Edwards MJ, McMasters KM: Utility of intraoperative frozen section analysis of sentinel lymph nodes in breast cancer. Am J Surg 2001, 182:609-615 (Pubitemid 34142680)
12. Gulec SA, Su J, O'Leary JP, Stolier A: Clinical utility of frozen section in sentinel node biopsy in breast cancer. Am Surg 2001, 67:529-532 (Pubitemid 33738844)
13. Hunt JL, Baloch ZW, LiVolsi VA: Sentinel lymph node evaluation for tumor metastasis. Semin Diagn Pathol 2002, 19:263-277 (Pubitemid 35365272)
14. Roberts CA, Beitsch PD, Litz CE, Hilton DS, Ewing GE, Clifford E, Taylor W, Hapke MR, Babaian A, Khalid I, Hall JD, Lindberg G, Molberg K, Saboorian H: Interpretive disparity among pathologists in breast sentinel lymph node evaluation. Am J Surg 2003, 186:324-329 (Pubitemid 38368603)
15. Turner RR, Weaver DL, Cserni G, Lester SC, Hirsch K, Elashoff DA, Fitzgibbons PL, Viale G, Mazzarol G, Ibarra JA, Schnitt SJ, Giuliano AE: Nodal stage classification for breast carcinoma: improving inter-observer reproducibility through standardized histologic criteria and image-based training. J Clin Oncol 2008, 26:258-263
16. Cserni G, Bianchi S, Boecker W, Decker T, Lacerda M, Rank F, Wells CA: Improving the reproducibility of diagnosing micrometastases and isolated tumor cells. Cancer 2005, 103:358-367 (Pubitemid 40129296)
17. Liefers GJ, Cleton-Jansen AM, van de Velde CJ, Hermans J, van Krieken JH, Cornelisse CJ, Tollenaar RA: Micrometastases and survival in stage II colorectal cancer. N Engl J Med 1998, 339:223-228 (Pubitemid 28350164)
18. Coello MC, Luketich JD, Litle VR, Godfrey TE: Prognostic significance of micrometastasis in non-small-cell lung cancer. Clin Lung Cancer 2004, 5:214-225 (Pubitemid 38312737)
19. Godfrey TE, Raja S, Finkelstein SD, Gooding WE, Kelly LA, Luketich JD: Prognostic value of quantitative reverse transcription-polymerase chain reaction in lymph node-negative esophageal cancer patients. Clin Cancer Res 2001, 7:4041-4048 (Pubitemid 34044625)
20. Hughes SJ, Xi L, Raja S, Gooding W, Cole DJ, Gillanders WE, Mikhitarian K, McCarty K, Silver S, Ching J, McMillan W, Luketich JD, Godfrey TE: A rapid, fully automated, molecular-based assay accurately analyzes sentinel lymph nodes for the presence of metastatic breast cancer. Ann Surg 2006, 243:389-398 (Pubitemid 43327772)
21. Keilholz U, Willhauck M, Rimoldi D, Brasseur F, Dummer W, Rass K, de VT, Blaheta J, Voit C, Lethe B, Burchill S: Reliability of reverse transcription- polymerase chain reaction (RT-PCR)-based assays for the detection of circulating tumour cells: a quality-assurance initiative of the EORTC Melanoma Cooperative Group. Eur J Cancer 1998, 34:750-753 (Pubitemid 28218955)
22. Bolufer P, Lo CF, Grimwade D, Barragan E, Diverio D, Cassinat B, Chomienne C, Gonzalez M, Colomer D, Gomez MT, Marugan I, Roman J, Delgado MD, Garcia-Marco JA, Bornstein R, Vizmanos JL, Martinez B, Jansen J, Villegas A, de Blas JM, Cabello P, Sanz MA: Variability in the levels of PML-RARα fusion transcripts detected by the laboratories participating in an external quality control program using several reverse transcription polymerase chain reaction protocols. Haematologica 2001, 86:570-576 (Pubitemid 32601100)
23. Reinhold U, Berkin C, Bosserhoff AK, Deutschmann A, Garbe C, Glaser R, Hein R, Krahn G, Peter RU, Rappl G, Schittek B, Seiter S, Ugurel S, Volkenandt M, Tilgen W: Interlaboratory evaluation of a new reverse transcriptase polymerase chain reaction-based enzyme-linked immunosorbent assay for the detection of circulating melanoma cells: a multicenter study of the Dermatologic Cooperative Oncology Group. J Clin Oncol 2001, 19:1723-1727 (Pubitemid 32230873)
24. Raja S, El Hefnawy T, Kelly LA, Chestney ML, Luketich JD, Godfrey TE: Temperature-controlled primer limit for multiplexing of rapid, quantitative reverse transcription-PCR assays: application to intraoperative cancer diagnostics. Clin Chem 2002, 48:1329-1337 (Pubitemid 34809843)
25. Raja S, Ching J, Xi L, Hughes SJ, Chang R, Wong W, McMillan W, Gooding WE, McCarty KS, Jr., Chestney M, Luketich JD, Godfrey TE: Technology for automated, rapid, and quantitative PCR or reverse transcription-PCR clinical testing. Clin Chem 2005, 51:882-890 (Pubitemid 40577926)
26. Gillanders WE, Mikhitarian K, Hebert R, Mauldin PD, Palesch Y, Walters C, Urist MM, Mann GB, Doherty G, Herrmann VM, Hill AD, Eremin O, El Sheemy M, Orr RK, Valle AA, Henderson MA, Dewitty RL, Sugg SL, Frykberg E, Yeh K, Bell RM, Metcalf JS, Elliott BM, Brothers T, Robison J, Mitas M, Cole DJ: Molecular detection of micrometastatic breast cancer in histopathology-negative axillary lymph nodes correlates with traditional predictors of prognosis: an interim analysis of a prospective multi-institutional cohort study. Ann Surg 2004, 239:828-837 (Pubitemid 38669433)
27. McIntosh MW, Pepe MS: Combining several screening tests: optimality of the risk score. Biometrics 2002, 58:657-664
28. Bostick PJ, Huynh KT, Sarantou T, Turner RR, Qi K, Giuliano AE, Hoon DS: Detection of metastases in sentinel lymph nodes of breast cancer patients by multiple-marker RT-PCR. Int J Cancer 1998, 79:645-651 (Pubitemid 28549479)
29. Bostick PJ, Morton DL, Turner RR, Huynh KT, Wang HJ, Elashoff R, Essner R, Hoon DS: Prognostic significance of occult metastases detected by sentinel lymphadenectomy and reverse transcriptase-polymerase chain reaction in early-stage melanoma patients. J Clin Oncol 1999, 17:3238-3244 (Pubitemid 29470658)
30. Mori M, Mimori K, Inoue H, Barnard GF, Tsuji K, Nanbara S, Ueo H, Akiyoshi T: Detection of cancer micrometastases in lymph nodes by reverse transcriptase-polymerase chain reaction. Cancer Res 1995, 55:3417-3420
31. Schoenfeld A, Luqmani Y, Smith D, O'Reilly S, Shousha S, Sinnett HD, Coombes RC: Detection of breast cancer micrometastases in axillary lymph nodes by using polymerase chain reaction. Cancer Res 1994, 54:2986-2990 (Pubitemid 24187784)
32. Tsujimoto M, Nakabayashi K, Yoshidome K, Kaneko T, Iwase T, Akiyama F, Kato Y, Tsuda H, Ueda S, Sato K, Tamaki Y, Noguchi S, Kataoka TR, Nakajima H, Komoike Y, Inaji H, Tsugawa K, Suzuki K, Nakamura S, Daitoh M, Otomo Y, Matsuura N: One-step nucleic acid amplification for intraoperative detection of lymph node metastasis in breast cancer patients. Clin Cancer Res 2007, 13:4807-4816 (Pubitemid 47294788)
33. Mikhitarian K, Martin RH, Mitas M, Mauldin PD, Palesch Y, Metcalf JS, Cole DJ, Gillanders WE: Molecular analysis improves sensitivity of breast sentinel lymph node biopsy: results of a multi-institutional prospective cohort study. Surgery 2005, 138:474-481 (Pubitemid 41415575)